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A method is presented for detecting the cellular location of specific DNA fractions. The technique involves the hybridization of a radioactive test DNA in solution to the stationary DNA of a cytological preparation. Sites of DNA binding are then detected by autoradiography. Experiments with DNA of the toad Xenopus are described.
Britten,
Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.
1968, Pubmed
Britten,
Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.
1968,
Pubmed Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968,
Pubmed CONGER,
A quick-freeze method for making smear slides permanent.
1953,
Pubmed Corneo,
Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids.
1968,
Pubmed COWIE,
HOMOLOGY BETWEEN BACTERIOPHAGE LAMBDA DNA AND E. COLI DNA.
1963,
Pubmed Evans,
Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis.
1968,
Pubmed
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Xenbase Flamm,
Some properties of the single strands isolated from the DNA of the nuclear satellite of the mouse (Mus musculus).
1969,
Pubmed Gall,
The genes for ribosomal RNA during oögenesis.
1969,
Pubmed Gall,
Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
1968,
Pubmed Gall,
Formation and detection of RNA-DNA hybrid molecules in cytological preparations.
1969,
Pubmed
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Xenbase HYER,
A MOLECULAR APPROACH IN THE SYSTEMATICS OF HIGHER ORGANISMS. DNA INTERACTIONS PROVIDE A BASIS FOR DETECTING COMMON POLYNUCLEOTIDE SEQUENCES AMONG DIVERSE ORGANISMS.
1964,
Pubmed John,
RNA-DNA hybrids at the cytological level.
1969,
Pubmed KIT,
Equilibrium sedimentation in density gradients of DNA preparations from animal tissues.
1961,
Pubmed MCCARTHY,
An approach to the measurement of genetic relatedness among organisms.
1963,
Pubmed SCHILDKRAUT,
The formation of hybrid DNA molecules and their use in studies of DNA homologies.
1961,
Pubmed Wallace,
Ribosomal cistrons and the nucleolar organizer.
1966,
Pubmed Westmoreland,
Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.
1969,
Pubmed
