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J Membr Biol
2007 Jan 01;2151:37-48. doi: 10.1007/s00232-007-9003-6.
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The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes.
Antoine AF, Montpellier C, Cailliau K, Browaeys-Poly E, Vilain JP, Dubuisson J.
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The Alphavirus Sindbis 6K protein is involved in several functions. It contributes to the processing and membrane insertion of E1 and PE2 viral envelope glycoproteins and to virus budding. It also permeabilizes Escherichia coli and mammalian cells. These viroporin-like properties have been proposed to help virus budding by modifying membrane permeabilities. We expressed Sindbis virus 6K cRNA in Xenopus oocytes to further characterize the effect of 6K on membrane conductances and permeabilization. Although no intrinsic channel properties were seen, cell shrinkage was observed within 24 h. Voltage-clamp experiments showed that 6K upregulated endogenous currents: a hyperpolarization-activated inward current (I (in)) and a calcium-dependent chloride current (I (Cl)). 6K was located at both the plasma and the endoplasmic reticulum membranes. The plasma membrane current upregulation likely results from disruption of the calcium homeostasis of the cell at the endoplasmic reticulum level. Indeed, 6K cRNA expression induced reticular calcium store depletion and capacitative calcium entry activation. By experimental modifications of the incubation medium, we showed that downstream of these events cell shrinkage resulted from a 6K -induced KCl efflux (I (Cl) upregulation leads to chloride efflux, which itself electrically drives potassium efflux), which was responsible for an osmotic water efflux. Our data confirm that 6K specifically triggers a sequential cascade of events that leads to cytoplasmic calcium elevation and cell permeabilization, which likely play a role in the Sindbis virus life cycle.
Aldabe,
Poliovirus protein 2BC increases cytosolic free calcium concentrations.
1997,
Pubmed Amasheh,
Further characteristics of the Ca(2+)-inactivated Cl(-) channel in Xenopus laevis oocytes.
1999,
Pubmed
,
Xenbase Bianchi,
The neurotoxic MEC-4(d) DEG/ENaC sodium channel conducts calcium: implications for necrosis initiation.
2004,
Pubmed
,
Xenbase Burgoyne,
Neuronal Ca2+-sensor proteins: multitalented regulators of neuronal function.
2004,
Pubmed Cailliau,
FGF receptor phosphotyrosine 766 is a target for Grb14 to inhibit MDA-MB-231 human breast cancer cell signaling.
2005,
Pubmed
,
Xenbase Carrasco,
Modification of membrane permeability by animal viruses.
1995,
Pubmed Carrère-Kremer,
Regulation of hepatitis C virus polyprotein processing by signal peptidase involves structural determinants at the p7 sequence junctions.
2004,
Pubmed Cheshenko,
Herpes simplex virus triggers activation of calcium-signaling pathways.
2003,
Pubmed Coady,
Effects of Vpu expression on Xenopus oocyte membrane conductance.
1998,
Pubmed
,
Xenbase Cooper,
Relationship between intracellular pH and chloride in Xenopus oocytes expressing the chloride channel ClC-0.
2003,
Pubmed
,
Xenbase Ewart,
The Vpu protein of human immunodeficiency virus type 1 forms cation-selective ion channels.
1996,
Pubmed Fischer,
Transmembrane peptide NB of influenza B: a simulation, structure, and conductance study.
2000,
Pubmed Garoff,
Assembly and entry mechanisms of Semliki Forest virus.
1994,
Pubmed Gillo,
Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca2+ entry.
1996,
Pubmed
,
Xenbase Gonzalez,
Viroporins.
2003,
Pubmed Griffin,
Emergence and virulence of encephalitogenic arboviruses.
2004,
Pubmed Griffin,
The p7 protein of hepatitis C virus forms an ion channel that is blocked by the antiviral drug, Amantadine.
2003,
Pubmed Irurzun,
Enhanced intracellular calcium concentration during poliovirus infection.
1995,
Pubmed Kelly,
Demonstrating the intrinsic ion channel activity of virally encoded proteins.
2003,
Pubmed Kuruma,
A hyperpolarization- and acid-activated nonselective cation current in Xenopus oocytes.
2000,
Pubmed
,
Xenbase Kuruma,
Dynamics of calcium regulation of chloride currents in Xenopus oocytes.
1999,
Pubmed
,
Xenbase Kuruma,
Bimodal control of a Ca(2+)-activated Cl(-) channel by different Ca(2+) signals.
2000,
Pubmed
,
Xenbase Lama,
Expression of poliovirus nonstructural proteins in Escherichia coli cells. Modification of membrane permeability induced by 2B and 3A.
1992,
Pubmed Lama,
Inducible expression of a toxic poliovirus membrane protein in Escherichia coli: comparative studies using different expression systems based on T7 promoters.
1992,
Pubmed Liljeström,
Internally located cleavable signal sequences direct the formation of Semliki Forest virus membrane proteins from a polyprotein precursor.
1991,
Pubmed Lusa,
Fate of the 6K membrane protein of Semliki Forest virus during virus assembly.
1991,
Pubmed Machaca,
Store-operated calcium entry inactivates at the germinal vesicle breakdown stage of Xenopus meiosis.
2000,
Pubmed
,
Xenbase Melton,
Alphavirus 6K proteins form ion channels.
2002,
Pubmed Nokta,
Ca2+ responses in cytomegalovirus-infected fibroblasts of human origin.
1987,
Pubmed Osman,
Wolframin expression induces novel ion channel activity in endoplasmic reticulum membranes and increases intracellular calcium.
2003,
Pubmed
,
Xenbase Paterson,
Influenza B virus BM2 protein is an oligomeric integral membrane protein expressed at the cell surface.
2003,
Pubmed Pavlović,
The hepatitis C virus p7 protein forms an ion channel that is inhibited by long-alkyl-chain iminosugar derivatives.
2003,
Pubmed Pérez,
Characterization of a membrane calcium pathway induced by rotavirus infection in cultured cells.
1999,
Pubmed Piller,
Extracellular HIV-1 virus protein R causes a large inward current and cell death in cultured hippocampal neurons: implications for AIDS pathology.
1998,
Pubmed Pinto,
Influenza virus M2 protein has ion channel activity.
1992,
Pubmed
,
Xenbase Plugge,
A potassium channel protein encoded by chlorella virus PBCV-1.
2000,
Pubmed
,
Xenbase Premkumar,
Cation-selective ion channels formed by p7 of hepatitis C virus are blocked by hexamethylene amiloride.
2004,
Pubmed Rice,
Production of infectious RNA transcripts from Sindbis virus cDNA clones: mapping of lethal mutations, rescue of a temperature-sensitive marker, and in vitro mutagenesis to generate defined mutants.
1987,
Pubmed Roth,
A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription.
1991,
Pubmed
,
Xenbase Sanderson,
Overexpression of the vaccinia virus A38L integral membrane protein promotes Ca2+ influx into infected cells.
1996,
Pubmed Sanz,
Semliki Forest virus 6K protein modifies membrane permeability after inducible expression in Escherichia coli cells.
1994,
Pubmed Sanz,
Interfacial domains in Sindbis virus 6K protein. Detection and functional characterization.
2003,
Pubmed Schubert,
Identification of an ion channel activity of the Vpu transmembrane domain and its involvement in the regulation of virus release from HIV-1-infected cells.
1996,
Pubmed
,
Xenbase Shainkin-Kestenbaum,
Inhibitory effect of the calcium antagonist, verapamil, on measles and vaccinia replication in cell culture.
1993,
Pubmed Shimbo,
Viral and cellular small integral membrane proteins can modify ion channels endogenous to Xenopus oocytes.
1995,
Pubmed
,
Xenbase Strauss,
The alphaviruses: gene expression, replication, and evolution.
1994,
Pubmed Sunstrom,
Ion channels formed by NB, an influenza B virus protein.
1996,
Pubmed Tang,
Beauvericin activates Ca2+-activated Cl- currents and induces cell deaths in Xenopus oocytes via influx of extracellular Ca2+.
2005,
Pubmed
,
Xenbase Tzounopoulos,
Induction of endogenous channels by high levels of heterologous membrane proteins in Xenopus oocytes.
1995,
Pubmed
,
Xenbase van Kuppeveld,
Coxsackievirus protein 2B modifies endoplasmic reticulum membrane and plasma membrane permeability and facilitates virus release.
1997,
Pubmed Weber,
Influence of extracellular Ca2+ on endogenous Cl- channels in Xenopus oocytes.
1995,
Pubmed
,
Xenbase Weber,
The Ca(2+)-induced leak current in Xenopus oocytes is indeed mediated through a Cl- channel.
1995,
Pubmed
,
Xenbase Yao,
Calcium current activated by depletion of calcium stores in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Zhang,
The ion selectivity of a membrane conductance inactivated by extracellular calcium in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
