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Biophys J
2006 Oct 15;918:2860-73. doi: 10.1529/biophysj.105.080242.
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New roles for a key glycine and its neighboring residue in potassium channel gating.
Rosenhouse-Dantsker A, Logothetis DE.
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Potassium channel activation regulates cellular excitability in cells such as neurons and heart. Ion channel activity relies on a switching mechanism between two conformations, the open and closed states, known as gating. It has been suggested that potassium channels are gated via a pivoted mechanism of the pore-lining helix. Our analysis suggests that hinging occurs at the residue immediately preceding the central glycine of the inner helix. Furthermore, we show that the highly conserved central glycine is necessary to prevent constraining interactions with critical residues in its vicinity, including those located in the selectivity filter. We show that such interactions can impair channel function, and that upon their removal channel activity can be restored.
Bright,
Conformational dynamics of helix S6 from Shaker potassium channel: simulation studies.
2002, Pubmed
Bright,
Conformational dynamics of helix S6 from Shaker potassium channel: simulation studies.
2002,
Pubmed Chakrabartty,
Large differences in the helix propensities of alanine and glycine.
1991,
Pubmed Chan,
Specific regions of heteromeric subunits involved in enhancement of G protein-gated K+ channel activity.
1997,
Pubmed
,
Xenbase Ding,
Investigating the putative glycine hinge in Shaker potassium channel.
2005,
Pubmed
,
Xenbase Domene,
Modeling of an ion channel in its open conformation.
2005,
Pubmed Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed He,
Identification of critical residues controlling G protein-gated inwardly rectifying K(+) channel activity through interactions with the beta gamma subunits of G proteins.
2002,
Pubmed He,
Identification of a potassium channel site that interacts with G protein betagamma subunits to mediate agonist-induced signaling.
1999,
Pubmed Heginbotham,
Mutations in the K+ channel signature sequence.
1994,
Pubmed
,
Xenbase Jiang,
The open pore conformation of potassium channels.
2002,
Pubmed Jiang,
X-ray structure of a voltage-dependent K+ channel.
2003,
Pubmed Jin,
The (beta)gamma subunits of G proteins gate a K(+) channel by pivoted bending of a transmembrane segment.
2002,
Pubmed
,
Xenbase Kirkpatrick,
Optimization by simulated annealing.
1983,
Pubmed Kleywegt,
Phi/psi-chology: Ramachandran revisited.
1996,
Pubmed Kuo,
Crystal structure of the potassium channel KirBac1.1 in the closed state.
2003,
Pubmed Labro,
Gating of shaker-type channels requires the flexibility of S6 caused by prolines.
2003,
Pubmed Liu,
Structure of the KcsA channel intracellular gate in the open state.
2001,
Pubmed Logothetis,
The beta gamma subunits of GTP-binding proteins activate the muscarinic K+ channel in heart.
,
Pubmed Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed Magidovich,
Conserved gating hinge in ligand- and voltage-dependent K+ channels.
2004,
Pubmed
,
Xenbase Perozo,
Structural rearrangements underlying K+-channel activation gating.
1999,
Pubmed Reuveny,
Activation of the cloned muscarinic potassium channel by G protein beta gamma subunits.
1994,
Pubmed
,
Xenbase Seebohm,
Differential roles of S6 domain hinges in the gating of KCNQ potassium channels.
2006,
Pubmed
,
Xenbase Tikhonov,
In silico activation of KcsA K+ channel by lateral forces applied to the C-termini of inner helices.
2004,
Pubmed Visiers,
Prokink: a protocol for numerical evaluation of helix distortions by proline.
2000,
Pubmed Vivaudou,
Probing the G-protein regulation of GIRK1 and GIRK4, the two subunits of the KACh channel, using functional homomeric mutants.
1997,
Pubmed
,
Xenbase Wuttke,
The new anticonvulsant retigabine favors voltage-dependent opening of the Kv7.2 (KCNQ2) channel by binding to its activation gate.
2005,
Pubmed
,
Xenbase Yakubovich,
Slow modal gating of single G protein-activated K+ channels expressed in Xenopus oocytes.
2000,
Pubmed
,
Xenbase Yifrach,
Energetics of pore opening in a voltage-gated K(+) channel.
2002,
Pubmed
,
Xenbase Zhang,
Gbetagamma-activated inwardly rectifying K(+) (GIRK) channel activation kinetics via Galphai and Galphao-coupled receptors are determined by Galpha-specific interdomain interactions that affect GDP release rates.
2004,
Pubmed
,
Xenbase Zhao,
A gating hinge in Na+ channels; a molecular switch for electrical signaling.
2004,
Pubmed
