Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2007 Aug 08;2615:3592-606. doi: 10.1038/sj.emboj.7601781.
Show Gene links
Show Anatomy links
XRab40 and XCullin5 form a ubiquitin ligase complex essential for the noncanonical Wnt pathway.
Lee RH, Iioka H, Ohashi M, Iemura S, Natsume T, Kinoshita N.
???displayArticle.abstract???
Rab GTPases are key regulators of intracellular membrane trafficking. We sought to elucidate the roles of Rab GTPases in Xenopus gastrulation, and found that a Xenopus homolog of Rab40 (XRab40) is required for normal gastrulation. XRab40 is localized at the Golgi apparatus and interacts with ElonginB/C and Cullin5 to form a ubiquitin ligase. XRab40/XCullin5 functions cooperatively and regulates the ubiquitination and localization of Rap2 GTPase. Furthermore, XRab40/XCullin5 regulates the membrane localization of Dishevelled (Dsh), a key signaling molecule in the Wnt pathway, through Rap2 and its effector Misshapen/Nck-interacting kinase (XMINK). XMINK interacts with Dsh, and is translocated to the plasma membrane by Wnt activation. We propose a novel signaling cascade consisting of XRab40/XCullin5, Rap2 and XMINK, which plays a crucial role in the regulation of the noncanonical Wnt pathway.
Angers,
The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
2006, Pubmed,
Xenbase
Angers,
The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
2006,
Pubmed
,
Xenbase Argon,
GRP94, an ER chaperone with protein and peptide binding properties.
1999,
Pubmed Axelrod,
Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways.
1998,
Pubmed
,
Xenbase Ayyub,
Cullin-5 plays multiple roles in cell fate specification and synapse formation during Drosophila development.
2005,
Pubmed Boutros,
Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling.
1998,
Pubmed Boutros,
Dishevelled: at the crossroads of divergent intracellular signaling pathways.
1999,
Pubmed
,
Xenbase Brannon,
Activation of Siamois by the Wnt pathway.
1996,
Pubmed
,
Xenbase Capelluto,
The DIX domain targets dishevelled to actin stress fibres and vesicular membranes.
2002,
Pubmed
,
Xenbase Carnac,
The homeobox gene Siamois is a target of the Wnt dorsalisation pathway and triggers organiser activity in the absence of mesoderm.
1996,
Pubmed
,
Xenbase Charrasse,
RhoA GTPase regulates M-cadherin activity and myoblast fusion.
2006,
Pubmed Cheyette,
Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation.
2002,
Pubmed
,
Xenbase Choi,
Rap2 is required for Wnt/beta-catenin signaling pathway in Xenopus early development.
2005,
Pubmed
,
Xenbase Choi,
Adenomatous polyposis coli is down-regulated by the ubiquitin-proteasome pathway in a process facilitated by Axin.
2004,
Pubmed
,
Xenbase d'Azzo,
E3 ubiquitin ligases as regulators of membrane protein trafficking and degradation.
2005,
Pubmed Deshaies,
SCF and Cullin/Ring H2-based ubiquitin ligases.
1999,
Pubmed Fu,
TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton.
1999,
Pubmed Fujita,
Hakai, a c-Cbl-like protein, ubiquitinates and induces endocytosis of the E-cadherin complex.
2002,
Pubmed Gaullier,
FYVE fingers bind PtdIns(3)P.
1998,
Pubmed Habas,
Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation.
2003,
Pubmed
,
Xenbase Haraguchi,
BAF is required for emerin assembly into the reforming nuclear envelope.
2001,
Pubmed Hilton,
Twenty proteins containing a C-terminal SOCS box form five structural classes.
1998,
Pubmed Hochstrasser,
Lingering mysteries of ubiquitin-chain assembly.
2006,
Pubmed Iioka,
Essential role of MARCKS in cortical actin dynamics during gastrulation movements.
2004,
Pubmed
,
Xenbase Kamura,
VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.
2004,
Pubmed Kile,
The SOCS box: a tale of destruction and degradation.
2002,
Pubmed Kinoshita,
PKC delta is essential for Dishevelled function in a noncanonical Wnt pathway that regulates Xenopus convergent extension movements.
2003,
Pubmed
,
Xenbase Köppen,
Coordinated cell-shape changes control epithelial movement in zebrafish and Drosophila.
2006,
Pubmed Kühl,
Non-canonical Wnt signaling in Xenopus: regulation of axis formation and gastrulation.
2002,
Pubmed
,
Xenbase Kwan,
Xbra functions as a switch between cell migration and convergent extension in the Xenopus gastrula.
2003,
Pubmed
,
Xenbase Levkowitz,
c-Cbl/Sli-1 regulates endocytic sorting and ubiquitination of the epidermal growth factor receptor.
1998,
Pubmed Li,
Dishevelled proteins lead to two signaling pathways. Regulation of LEF-1 and c-Jun N-terminal kinase in mammalian cells.
1999,
Pubmed Machida,
Mitogen-activated protein kinase kinase kinase kinase 4 as a putative effector of Rap2 to activate the c-Jun N-terminal kinase.
2004,
Pubmed Masuhara,
Cloning and characterization of novel CIS family genes.
1997,
Pubmed McKendry,
LEF-1/TCF proteins mediate wnt-inducible transcription from the Xenopus nodal-related 3 promoter.
1997,
Pubmed
,
Xenbase Metzelaar,
CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells.
1991,
Pubmed Miller,
Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation.
1999,
Pubmed
,
Xenbase Miyazaki,
NEDL1, a novel ubiquitin-protein isopeptide ligase for dishevelled-1, targets mutant superoxide dismutase-1.
2004,
Pubmed Natsume,
A direct nanoflow liquid chromatography-tandem mass spectrometry system for interaction proteomics.
2002,
Pubmed Palacios,
Lysosomal targeting of E-cadherin: a unique mechanism for the down-regulation of cell-cell adhesion during epithelial to mesenchymal transitions.
2005,
Pubmed Paricio,
The Drosophila STE20-like kinase misshapen is required downstream of the Frizzled receptor in planar polarity signaling.
1999,
Pubmed Park,
Subcellular localization and signaling properties of dishevelled in developing vertebrate embryos.
2005,
Pubmed
,
Xenbase Patki,
A functional PtdIns(3)P-binding motif.
1998,
Pubmed Petroski,
Function and regulation of cullin-RING ubiquitin ligases.
2005,
Pubmed Rodriguez-Gabin,
Vesicle transport in oligodendrocytes: probable role of Rab40c protein.
2004,
Pubmed Rothbächer,
Dishevelled phosphorylation, subcellular localization and multimerization regulate its role in early embryogenesis.
2000,
Pubmed
,
Xenbase Scholpp,
Endocytosis controls spreading and effective signaling range of Fgf8 protein.
2004,
Pubmed Schwarz-Romond,
The Wnt signalling effector Dishevelled forms dynamic protein assemblies rather than stable associations with cytoplasmic vesicles.
2005,
Pubmed Shih,
Cell motility driving mediolateral intercalation in explants of Xenopus laevis.
1992,
Pubmed
,
Xenbase Simons,
Inversin, the gene product mutated in nephronophthisis type II, functions as a molecular switch between Wnt signaling pathways.
2005,
Pubmed
,
Xenbase Smith,
Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction.
1991,
Pubmed
,
Xenbase Tada,
Non-canonical Wnt signalling and regulation of gastrulation movements.
2002,
Pubmed
,
Xenbase Taira,
The Traf2- and Nck-interacting kinase as a putative effector of Rap2 to regulate actin cytoskeleton.
2004,
Pubmed Ulrich,
Wnt11 functions in gastrulation by controlling cell cohesion through Rab5c and E-cadherin.
2005,
Pubmed Voigt,
A dominant-negative form of the E3 ubiquitin ligase Cullin-1 disrupts the correct allocation of cell fate in the neural crest lineage.
2006,
Pubmed
,
Xenbase Wallingford,
Convergent extension: the molecular control of polarized cell movement during embryonic development.
2002,
Pubmed
,
Xenbase Wharton,
Runnin' with the Dvl: proteins that associate with Dsh/Dvl and their significance to Wnt signal transduction.
2003,
Pubmed Yamaguchi,
Golgi retention mechanism of beta-1,4-galactosyltransferase. Membrane-spanning domain-dependent homodimerization and association with alpha- and beta-tubulins.
1995,
Pubmed Yamanaka,
JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates.
2002,
Pubmed
,
Xenbase
