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Neurotransmission during development regulates synaptic maturation in neural circuits, but the contribution of different neurotransmitter systems is unclear. We investigated the role of GABAA receptor-mediated Cl- conductances in the development of synaptic responses in the Xenopus visual system. Intracellular Cl- concentration ([Cl-]i) was found to be high in immature tectal neurons and then falls over a period of several weeks. GABAergic synapses are present at early stages of tectal development and, when activated by optic nerve stimulation or visual stimuli, induce sustained depolarizing Cl- conductances that facilitate retinotectal transmission by NMDA receptors. To test whether depolarizing GABAergic inputs cooperate with NMDA receptors during activity-dependent maturation of glutamatergic synapses, we prematurely reduced [Cl-]i in tectal neurons in vivo by expressing the Cl- transporter KCC2. This blocked the normal developmental increase in AMPA receptor-mediated retinotectal transmission and increased GABAergic synaptic input to tectal neurons. Therefore, depolarizing GABAergic transmission plays a pivotal role in the maturation of excitatory transmission and controls the balance of excitation and inhibition in the developing retinotectal circuit.
Aamodt,
Chronic NMDA exposure accelerates development of GABAergic inhibition in the superior colliculus.
2000, Pubmed
Aamodt,
Chronic NMDA exposure accelerates development of GABAergic inhibition in the superior colliculus.
2000,
Pubmed Aizenman,
Visually driven regulation of intrinsic neuronal excitability improves stimulus detection in vivo.
2003,
Pubmed
,
Xenbase Artola,
Different voltage-dependent thresholds for inducing long-term depression and long-term potentiation in slices of rat visual cortex.
1990,
Pubmed Ascher,
The role of divalent cations in the N-methyl-D-aspartate responses of mouse central neurones in culture.
1988,
Pubmed Ben-Ari,
Excitatory actions of gaba during development: the nature of the nurture.
2002,
Pubmed Ben-Ari,
Giant synaptic potentials in immature rat CA3 hippocampal neurones.
1989,
Pubmed Chih,
Control of excitatory and inhibitory synapse formation by neuroligins.
2005,
Pubmed Chudotvorova,
Early expression of KCC2 in rat hippocampal cultures augments expression of functional GABA synapses.
2005,
Pubmed Constantine-Paton,
Patterned activity, synaptic convergence, and the NMDA receptor in developing visual pathways.
1990,
Pubmed Cummings,
Ca2+ signaling requirements for long-term depression in the hippocampus.
1996,
Pubmed Ehrlich,
Shift from depolarizing to hyperpolarizing glycine action in rat auditory neurones is due to age-dependent Cl- regulation.
1999,
Pubmed Gabernet,
Somatosensory integration controlled by dynamic thalamocortical feed-forward inhibition.
2005,
Pubmed Graf,
Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins.
2004,
Pubmed Hensch,
Local GABA circuit control of experience-dependent plasticity in developing visual cortex.
1998,
Pubmed Holt,
Does timing of axon outgrowth influence initial retinotectal topography in Xenopus?
1984,
Pubmed
,
Xenbase Hübner,
Disruption of KCC2 reveals an essential role of K-Cl cotransport already in early synaptic inhibition.
2001,
Pubmed Isaac,
Silent synapses during development of thalamocortical inputs.
1997,
Pubmed Jahr,
Voltage dependence of NMDA-activated macroscopic conductances predicted by single-channel kinetics.
1990,
Pubmed Khalilov,
In vitro formation of a secondary epileptogenic mirror focus by interhippocampal propagation of seizures.
2003,
Pubmed Kovalchuk,
NMDA receptor-mediated subthreshold Ca(2+) signals in spines of hippocampal neurons.
2000,
Pubmed Kyrozis,
Perforated-patch recording with gramicidin avoids artifactual changes in intracellular chloride concentration.
1995,
Pubmed Lee,
KCC2 expression in immature rat cortical neurons is sufficient to switch the polarity of GABA responses.
2005,
Pubmed Leinekugel,
Ca2+ oscillations mediated by the synergistic excitatory actions of GABA(A) and NMDA receptors in the neonatal hippocampus.
1997,
Pubmed Liu,
Local structural balance and functional interaction of excitatory and inhibitory synapses in hippocampal dendrites.
2004,
Pubmed Mayer,
Voltage-dependent block by Mg2+ of NMDA responses in spinal cord neurones.
,
Pubmed McLean,
Bidirectional plasticity expressed by GABAergic synapses in the neonatal rat hippocampus.
1996,
Pubmed Murthy,
Inactivity produces increases in neurotransmitter release and synapse size.
2001,
Pubmed Nelson,
Orientation selectivity of cortical neurons during intracellular blockade of inhibition.
1994,
Pubmed Niell,
In vivo imaging of synapse formation on a growing dendritic arbor.
2004,
Pubmed Owens,
Is there more to GABA than synaptic inhibition?
2002,
Pubmed Owens,
Excitatory GABA responses in embryonic and neonatal cortical slices demonstrated by gramicidin perforated-patch recordings and calcium imaging.
1996,
Pubmed Pettit,
Potentiated transmission and prevention of further LTP by increased CaMKII activity in postsynaptic hippocampal slice neurons.
1994,
Pubmed Pouille,
Enforcement of temporal fidelity in pyramidal cells by somatic feed-forward inhibition.
2001,
Pubmed Prange,
A balance between excitatory and inhibitory synapses is controlled by PSD-95 and neuroligin.
2004,
Pubmed Rajan,
NMDA receptor activity stabilizes presynaptic retinotectal axons and postsynaptic optic tectal cell dendrites in vivo.
1999,
Pubmed
,
Xenbase Rivera,
Mechanism of activity-dependent downregulation of the neuron-specific K-Cl cotransporter KCC2.
2004,
Pubmed Rivera,
The K+/Cl- co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation.
1999,
Pubmed Robinson,
Injection of digitally synthesized synaptic conductance transients to measure the integrative properties of neurons.
1993,
Pubmed Rohrbough,
Regulation of intracellular Cl- levels by Na(+)-dependent Cl- cotransport distinguishes depolarizing from hyperpolarizing GABAA receptor-mediated responses in spinal neurons.
1996,
Pubmed
,
Xenbase Ruthazer,
Control of axon branch dynamics by correlated activity in vivo.
2003,
Pubmed
,
Xenbase Schiller,
NMDA spikes in basal dendrites of cortical pyramidal neurons.
2000,
Pubmed Sharp,
The dynamic clamp: artificial conductances in biological neurons.
1993,
Pubmed Shi,
Developmental depression of glutamate neurotransmission by chronic low-level activation of NMDA receptors.
2001,
Pubmed Shi,
Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation.
1999,
Pubmed Strange,
Dependence of KCC2 K-Cl cotransporter activity on a conserved carboxy terminus tyrosine residue.
2000,
Pubmed
,
Xenbase Tao,
Activity-dependent matching of excitatory and inhibitory inputs during refinement of visual receptive fields.
2005,
Pubmed
,
Xenbase Tao,
Emergence of input specificity of ltp during development of retinotectal connections in vivo.
2001,
Pubmed
,
Xenbase Thiagarajan,
alpha- and betaCaMKII. Inverse regulation by neuronal activity and opposing effects on synaptic strength.
2002,
Pubmed Watt,
A proportional but slower NMDA potentiation follows AMPA potentiation in LTP.
2004,
Pubmed Wehr,
Balanced inhibition underlies tuning and sharpens spike timing in auditory cortex.
2003,
Pubmed Woodin,
Coincident pre- and postsynaptic activity modifies GABAergic synapses by postsynaptic changes in Cl- transporter activity.
2003,
Pubmed Wu,
Dendritic dynamics in vivo change during neuronal maturation.
1999,
Pubmed
,
Xenbase Wu,
Time-lapse in vivo imaging of the morphological development of Xenopus optic tectal interneurons.
2003,
Pubmed
,
Xenbase Wu,
Maturation of a central glutamatergic synapse.
1996,
Pubmed
,
Xenbase Xiao,
Creation of AMPA-silent synapses in the neonatal hippocampus.
2004,
Pubmed Zhang,
A critical window for cooperation and competition among developing retinotectal synapses.
1998,
Pubmed
,
Xenbase Zhu,
Acute versus chronic NMDA receptor blockade and synaptic AMPA receptor delivery.
2002,
Pubmed
