Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Mitochondria with high membrane potential (DeltaPsi(m)) are enriched in the presynaptic nerve terminal at vertebrate neuromuscular junctions, but the exact function of these localized synaptic mitochondria remains unclear. Here, we investigated the correlation between mitochondrial DeltaPsi(m) and the development of synaptic specializations. Using mitochondrial DeltaPsi(m)-sensitive probe JC-1, we found that DeltaPsi(m) in Xenopus spinal neurons could be reversibly elevated by creatine and suppressed by FCCP. Along naïve neurites, preexisting synaptic vesicle (SV) clusters were positively correlated with mitochondrial DeltaPsi(m), suggesting a potential regulatory role of mitochondrial activity in synaptogenesis. Indicating a specific role of mitochondrial activity in presynaptic development, mitochondrial ATP synthase inhibitor oligomycin, but not mitochondrial Na(+)/Ca(2+) exchanger inhibitor CGP-37157, inhibited the clustering of SVs induced by growth factor-coated beads. Local F-actin assembly induced along spinal neurites by beads was suppressed by FCCP or oligomycin. Our results suggest that a key role of presynaptic mitochondria is to provide ATP for the assembly of actin cytoskeleton involved in the assembly of the presynaptic specialization including the clustering of SVs and mitochondria themselves.
Alnaes,
On the role of mitochondria in transmitter release from motor nerve terminals.
1975, Pubmed
Alnaes,
On the role of mitochondria in transmitter release from motor nerve terminals.
1975,
Pubmed Ankarcrona,
Glutamate-induced neuronal death: a succession of necrosis or apoptosis depending on mitochondrial function.
1995,
Pubmed Baron,
CGP37157 modulates mitochondrial Ca2+ homeostasis in cultured rat dorsal root ganglion neurons.
1997,
Pubmed Benfenati,
Computer modeling of synapsin I binding to synaptic vesicles and F-actin: implications for regulation of neurotransmitter release.
1991,
Pubmed Bernstein,
Actin-ATP hydrolysis is a major energy drain for neurons.
2003,
Pubmed Bertina,
The mechanism of inhibition by oligomycin of oxidative phosphorylation in mitochondria.
1974,
Pubmed Bixby,
The expression and localization of synaptic vesicle antigens at neuromuscular junctions in vitro.
1985,
Pubmed Bloom,
Colocalization of synapsin and actin during synaptic vesicle recycling.
2003,
Pubmed Bubb,
Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin.
1994,
Pubmed Buckman,
Spontaneous changes in mitochondrial membrane potential in cultured neurons.
2001,
Pubmed Cai,
Syntabulin-mediated anterograde transport of mitochondria along neuronal processes.
2005,
Pubmed Cox,
Selectivity of inhibition of Na(+)-Ca2+ exchange of heart mitochondria by benzothiazepine CGP-37157.
1993,
Pubmed Dai,
Presynaptic differentiation induced in cultured neurons by local application of basic fibroblast growth factor.
1995,
Pubmed
,
Xenbase Dai,
Dynamics of synaptic vesicles in cultured spinal cord neurons in relationship to synaptogenesis.
1996,
Pubmed
,
Xenbase Dai,
The actin-driven movement and formation of acetylcholine receptor clusters.
2000,
Pubmed
,
Xenbase Dillon,
The actin cytoskeleton: integrating form and function at the synapse.
2005,
Pubmed Evans,
Vesicle-associated brain myosin-V can be activated to catalyze actin-based transport.
1998,
Pubmed Guo,
The GTPase dMiro is required for axonal transport of mitochondria to Drosophila synapses.
2005,
Pubmed Herrera,
Ultrastructural correlates of naturally occurring differences in transmitter release efficacy in frog motor nerve terminals.
1985,
Pubmed Hirokawa,
The cytoskeletal architecture of the presynaptic terminal and molecular structure of synapsin 1.
1989,
Pubmed Hollenbeck,
The pattern and mechanism of mitochondrial transport in axons.
1996,
Pubmed Ishida,
Compartmentation of ATP synthesis and utilization in smooth muscle: roles of aerobic glycolysis and creatine kinase.
1994,
Pubmed Kuromi,
Two distinct pools of synaptic vesicles in single presynaptic boutons in a temperature-sensitive Drosophila mutant, shibire.
1998,
Pubmed Lee,
Mitochondrial clustering at the vertebrate neuromuscular junction during presynaptic differentiation.
2006,
Pubmed
,
Xenbase Lee,
Direct observation of brownian motion of lipids in a membrane.
1991,
Pubmed Leyssens,
The relationship between mitochondrial state, ATP hydrolysis, [Mg2+]i and [Ca2+]i studied in isolated rat cardiomyocytes.
1996,
Pubmed Li,
The importance of dendritic mitochondria in the morphogenesis and plasticity of spines and synapses.
2004,
Pubmed Miller,
Axonal mitochondrial transport and potential are correlated.
2004,
Pubmed Möller,
Creatine transport in cultured cells of rat and mouse brain.
1989,
Pubmed Morris,
Axonal transport of mitochondria along microtubules and F-actin in living vertebrate neurons.
1995,
Pubmed Morris,
The regulation of bidirectional mitochondrial transport is coordinated with axonal outgrowth.
1993,
Pubmed Nguyen,
Synaptic physiology and mitochondrial function in crayfish tonic and phasic motor neurons.
1997,
Pubmed Nicholls,
Mitochondria and neuronal survival.
2000,
Pubmed Peng,
Association of cortactin with developing neuromuscular specializations.
1997,
Pubmed
,
Xenbase Peng,
Tissue culture of Xenopus neurons and muscle cells as a model for studying synaptic induction.
1991,
Pubmed
,
Xenbase Poot,
Analysis of mitochondrial morphology and function with novel fixable fluorescent stains.
1996,
Pubmed Richards,
Effects of wortmannin and latrunculin A on slow endocytosis at the frog neuromuscular junction.
2004,
Pubmed Rizzoli,
Monitoring synaptic vesicle recycling in frog motor nerve terminals with FM dyes.
2003,
Pubmed Rizzoli,
Synaptic vesicle pools.
2005,
Pubmed Robitaille,
Non-uniform release at the frog neuromuscular junction: evidence of morphological and physiological plasticity.
1987,
Pubmed Scanlon,
Pharmacological investigation of mitochondrial ca(2+) transport in central neurons: studies with CGP-37157, an inhibitor of the mitochondrial Na(+)-Ca(2+) exchanger.
2000,
Pubmed Schafer,
Visualization and molecular analysis of actin assembly in living cells.
1998,
Pubmed Smiley,
Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.
1991,
Pubmed Stowers,
Axonal transport of mitochondria to synapses depends on milton, a novel Drosophila protein.
2002,
Pubmed Symons,
Control of actin polymerization in live and permeabilized fibroblasts.
1991,
Pubmed Verstreken,
Synaptic mitochondria are critical for mobilization of reserve pool vesicles at Drosophila neuromuscular junctions.
2005,
Pubmed White,
Mitochondrial depolarization in glutamate-stimulated neurons: an early signal specific to excitotoxin exposure.
1996,
Pubmed Yang,
Ca2+ influx-independent synaptic potentiation mediated by mitochondrial Na(+)-Ca2+ exchanger and protein kinase C.
2003,
Pubmed
,
Xenbase
