Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Transgenic Res
2008 Apr 01;172:265-79. doi: 10.1007/s11248-007-9152-5.
Show Gene links
Show Anatomy links
Generation and characterization of transgenic zebrafish lines using different ubiquitous promoters.
Burket CT, Montgomery JE, Thummel R, Kassen SC, LaFave MC, Langenau DM, Zon LI, Hyde DR.
???displayArticle.abstract???
Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly the central nervous system. To overcome this limitation, we cloned the promoters of four zebrafish genes that likely are transcribed ubiquitously throughout development and into the adult. These four genes are the TATA box binding protein gene, the taube nuss-like gene, the eukaryotic elongation factor 1-gamma gene, and the beta-actin-1 gene. We PCR amplified approximately 2.5 kb upstream of the putative translational start site of each gene and cloned each into a Tol2 expression vector that contains the EGFP reporter transgene. We used these four Tol2 vectors to independently generate stable transgenic fish lines for analysis of transgene expression during development and in the adult. We demonstrated that all four promoters drive a very broad pattern of EGFP expression throughout development and the adult. Using the retina as a well-characterized component of the CNS, all four promoters appeared to drive EGFP expression in all neuronal and non-neuronal cells of the adult retina. In contrast, the h2afv promoter failed to express EGFP in the adult retina. When we examined EGFP expression in the various cells of the blood cell lineage, we observed that all four promoters exhibited a more heterogenous expression pattern than either the XlEef1a1 or h2afv promoters. While these four ubiquitous promoters did not express EGFP in all the adult blood cells, they did express EGFP throughout the CNS and in broader expression patterns in the adult than either the XlEef1a1 or h2afv promoters. For these reasons, these four promoters will be valuable tools for expressing transgenes in adult zebrafish.
???displayArticle.pubmedLink???
17968670 ???displayArticle.pmcLink???PMC3660017 ???displayArticle.link???Transgenic Res ???displayArticle.grants???[+]
Amsterdam,
The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos.
1995, Pubmed,
Xenbase
Amsterdam,
The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos.
1995,
Pubmed
,
Xenbase Amsterdam,
Requirements for green fluorescent protein detection in transgenic zebrafish embryos.
1996,
Pubmed
,
Xenbase Bai,
Matrix metalloproteinase expression and function during fin regeneration in zebrafish: analysis of MT1-MMP, MMP2 and TIMP2.
2005,
Pubmed Bretaud,
A choice behavior for morphine reveals experience-dependent drug preference and underlying neural substrates in developing larval zebrafish.
2007,
Pubmed Brinster,
Introns increase transcriptional efficiency in transgenic mice.
1988,
Pubmed Chalfie,
Green fluorescent protein as a marker for gene expression.
1994,
Pubmed Collas,
Modulation of plasmid DNA methylation and expression in zebrafish embryos.
1998,
Pubmed Dong,
Transgene manipulation in zebrafish by using recombinases.
2004,
Pubmed Feng,
Heat-shock induction of T-cell lymphoma/leukaemia in conditional Cre/lox-regulated transgenic zebrafish.
2007,
Pubmed Fimbel,
Regeneration of inner retinal neurons after intravitreal injection of ouabain in zebrafish.
2007,
Pubmed Furutani-Seiki,
Neural degeneration mutants in the zebrafish, Danio rerio.
1996,
Pubmed Gerhard,
Small laboratory fish as models for aging research.
2007,
Pubmed Gibbs,
An in vivo screen for the luciferase transgene in zebrafish.
1994,
Pubmed Gibbs,
GFP as a Genetic Marker Scorable Throughout the Life Cycle of Transgenic Zebra Fish.
2000,
Pubmed Grunwald,
A neural degeneration mutation that spares primary neurons in the zebrafish.
1988,
Pubmed Herrera,
Defining the cell lineages of the islets of Langerhans using transgenic mice.
2002,
Pubmed Herrera,
Pancreatic cell lineage analyses in mice.
2002,
Pubmed Herrera,
Two transgenic approaches to define the cell lineages in endocrine pancreas development.
1998,
Pubmed Higashijima,
High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin.
1997,
Pubmed Hirsch,
Xenopus tropicalis transgenic lines and their use in the study of embryonic induction.
2002,
Pubmed
,
Xenbase Iovine,
Mutations in connexin43 (GJA1) perturb bone growth in zebrafish fins.
2005,
Pubmed Johnson,
pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos.
1994,
Pubmed
,
Xenbase Johnson,
A Xenopus laevis gene encoding EF-1 alpha S, the somatic form of elongation factor 1 alpha: sequence, structure, and identification of regulatory elements required for embryonic transcription.
1995,
Pubmed
,
Xenbase Ju,
Faithful expression of green fluorescent protein (GFP) in transgenic zebrafish embryos under control of zebrafish gene promoters.
1999,
Pubmed Kassen,
Time course analysis of gene expression during light-induced photoreceptor cell death and regeneration in albino zebrafish.
2007,
Pubmed Kawakami,
Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage.
2000,
Pubmed Kawakami,
Identification of the Tol2 transposase of the medaka fish Oryzias latipes that catalyzes excision of a nonautonomous Tol2 element in zebrafish Danio rerio.
1999,
Pubmed Kawakami,
Excision of the tol2 transposable element of the medaka fish, Oryzias latipes, in zebrafish, Danio rerio.
1998,
Pubmed Kennedy,
Isolation of a zebrafish rod opsin promoter to generate a transgenic zebrafish line expressing enhanced green fluorescent protein in rod photoreceptors.
2001,
Pubmed Kim,
Lrrc10 is required for early heart development and function in zebrafish.
2007,
Pubmed Kondo,
Bone morphogenetic proteins in the early development of zebrafish.
2007,
Pubmed
,
Xenbase Langenau,
Cre/lox-regulated transgenic zebrafish model with conditional myc-induced T cell acute lymphoblastic leukemia.
2005,
Pubmed Lee,
Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration.
2005,
Pubmed Le Hir,
How introns influence and enhance eukaryotic gene expression.
2003,
Pubmed Lesaffre,
Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish.
2007,
Pubmed Lewis,
From cells to circuits: development of the zebrafish spinal cord.
2003,
Pubmed Long,
GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene.
1997,
Pubmed Malicki,
Mutations affecting development of the zebrafish ear.
1996,
Pubmed Malicki,
Mutations affecting development of the zebrafish retina.
1996,
Pubmed Meng,
Promoter analysis in living zebrafish embryos identifies a cis-acting motif required for neuronal expression of GATA-2.
1997,
Pubmed Muto,
Forward genetic analysis of visual behavior in zebrafish.
2005,
Pubmed Nakatani,
Cellular and molecular processes of regeneration, with special emphasis on fish fins.
2007,
Pubmed Ninkovic,
The zebrafish as a model system for assessing the reinforcing properties of drugs of abuse.
2006,
Pubmed Orger,
Behavioral screening assays in zebrafish.
2004,
Pubmed Pan,
Demonstration of site-directed recombination in transgenic zebrafish using the Cre/loxP system.
2005,
Pubmed Pauls,
A zebrafish histone variant H2A.F/Z and a transgenic H2A.F/Z:GFP fusion protein for in vivo studies of embryonic development.
2001,
Pubmed Poss,
Heart regeneration in zebrafish.
2002,
Pubmed Schmitt,
Early eye morphogenesis in the zebrafish, Brachydanio rerio.
1994,
Pubmed Schmitt,
Early retinal development in the zebrafish, Danio rerio: light and electron microscopic analyses.
1999,
Pubmed Thummel,
Inhibition of zebrafish fin regeneration using in vivo electroporation of morpholinos against fgfr1 and msxb.
2006,
Pubmed Thummel,
Two different transgenes to study gene silencing and re-expression during zebrafish caudal fin and retinal regeneration.
2006,
Pubmed
,
Xenbase Thummel,
Cre-mediated site-specific recombination in zebrafish embryos.
2005,
Pubmed Tomasiewicz,
Transgenic zebrafish model of neurodegeneration.
2002,
Pubmed Traver,
Cellular dissection of zebrafish hematopoiesis.
2004,
Pubmed Udvadia,
Windows into development: historic, current, and future perspectives on transgenic zebrafish.
2003,
Pubmed Vihtelic,
Lens opacity and photoreceptor degeneration in the zebrafish lens opaque mutant.
2005,
Pubmed Vihtelic,
Arrested differentiation and epithelial cell degeneration in zebrafish lens mutants.
2001,
Pubmed Vihtelic,
Light-induced rod and cone cell death and regeneration in the adult albino zebrafish (Danio rerio) retina.
2000,
Pubmed Whitehead,
fgf20 is essential for initiating zebrafish fin regeneration.
2005,
Pubmed Yu,
Synchronizing multiphasic circadian rhythms of rhodopsin promoter expression in rod photoreceptor cells.
2007,
Pubmed Zhang,
The expression of novel membrane-type matrix metalloproteinase isoforms is required for normal development of zebrafish embryos.
2003,
Pubmed
