Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell
2007 Oct 26;282:240-52. doi: 10.1016/j.molcel.2007.09.026.
Show Gene links
Show Anatomy links
U2 snRNP binds intronless histone pre-mRNAs to facilitate U7-snRNP-dependent 3' end formation.
Friend K, Lovejoy AF, Steitz JA.
???displayArticle.abstract???
In metazoa, pre-mRNA 3' end formation occurs via two pathways: cleavage/polyadenylation for the majority of RNA polymerase II transcripts and U7-snRNP-dependent cleavage for replication-dependent histone pre-mRNAs. An RNA element derived from a replication-dependent histone gene affects multiple steps of pre-mRNA processing. Here, we demonstrate that a portion of this RNA element, present in the majority of histone mRNAs, stimulates U7-snRNP-dependent cleavage. Surprisingly, this element binds U2 snRNP, although it is derived from an intronless mRNA. Specifically, SF3b, a U2 and U12-snRNP component, contacts the RNA element both in vitro and in vivo in conjunction with hPrp43, a DEAH-box helicase. Tethering either U2 or U12 snRNP to histone pre-mRNA substrates stimulates U7-snRNP-dependent cleavage in vitro and in vivo. Finally, we show that U2 snRNP associates with histone pre-mRNAs in vivo. We conclude that U2 snRNP plays a nonsplicing role in histone mRNA maturation.
Arenas,
Prp43: An RNA helicase-like factor involved in spliceosome disassembly.
1997, Pubmed
Arenas,
Prp43: An RNA helicase-like factor involved in spliceosome disassembly.
1997,
Pubmed Barnard,
Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation.
2004,
Pubmed
,
Xenbase Behrens,
Small nuclear ribonucleoprotein (RNP) U2 contains numerous additional proteins and has a bipartite RNP structure under splicing conditions.
1993,
Pubmed Blanchette,
Genome-wide analysis reveals an unexpected function for the Drosophila splicing factor U2AF50 in the nuclear export of intronless mRNAs.
2004,
Pubmed Bond,
Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.
1991,
Pubmed Boudonck,
The movement of coiled bodies visualized in living plant cells by the green fluorescent protein.
1999,
Pubmed Brosi,
Separation of splicing factor SF3 into two components and purification of SF3a activity.
1993,
Pubmed Cass,
The SF3b155 N-terminal domain is a scaffold important for splicing.
2006,
Pubmed Cavaloc,
Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family.
1994,
Pubmed Cooke,
The cap and the 3' splice site similarly affect polyadenylation efficiency.
1996,
Pubmed Das,
Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.
1999,
Pubmed DeBry,
Selection on silent sites in the rodent H3 histone gene family.
1994,
Pubmed Dettwiler,
Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization.
2004,
Pubmed Dominski,
The polyadenylation factor CPSF-73 is involved in histone-pre-mRNA processing.
2005,
Pubmed Dönmez,
Modified nucleotides at the 5' end of human U2 snRNA are required for spliceosomal E-complex formation.
2004,
Pubmed Fouraux,
The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase.
2002,
Pubmed Frey,
Coiled bodies contain U7 small nuclear RNA and associate with specific DNA sequences in interphase human cells.
1995,
Pubmed Gama-Carvalho,
Targeting of U2AF65 to sites of active splicing in the nucleus.
1997,
Pubmed Gozani,
Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A.
1996,
Pubmed Hamm,
Functional analysis of mutant Xenopus U2 snRNAs.
1989,
Pubmed
,
Xenbase Hirose,
A spliceosomal intron binding protein, IBP160, links position-dependent assembly of intron-encoded box C/D snoRNP to pre-mRNA splicing.
2006,
Pubmed Hofmann,
Symplekin, a constitutive protein of karyo- and cytoplasmic particles involved in mRNA biogenesis in Xenopus laevis oocytes.
2002,
Pubmed
,
Xenbase Huang,
Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA.
2001,
Pubmed
,
Xenbase Huang,
Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing.
1999,
Pubmed Huang,
SR splicing factors serve as adapter proteins for TAP-dependent mRNA export.
2003,
Pubmed
,
Xenbase Kolev,
Symplekin and multiple other polyadenylation factors participate in 3'-end maturation of histone mRNAs.
2005,
Pubmed
,
Xenbase Krämer,
Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo.
1994,
Pubmed Krämer,
Structure-function analysis of the U2 snRNP-associated splicing factor SF3a.
2005,
Pubmed Kyburz,
Direct interactions between subunits of CPSF and the U2 snRNP contribute to the coupling of pre-mRNA 3' end processing and splicing.
2006,
Pubmed Lebaron,
The splicing ATPase prp43p is a component of multiple preribosomal particles.
2005,
Pubmed Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase Lutz,
Direct interaction of the U1 snRNP-A protein with the upstream efficiency element of the SV40 late polyadenylation signal.
1994,
Pubmed Mandel,
Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease.
2006,
Pubmed Martin,
Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome.
2002,
Pubmed Marzluff,
U2 snRNP: not just for poly(A) mRNAs.
2007,
Pubmed Marzluff,
The human and mouse replication-dependent histone genes.
2002,
Pubmed Marzluff,
Metazoan replication-dependent histone mRNAs: a distinct set of RNA polymerase II transcripts.
2005,
Pubmed McCracken,
SRm160 splicing coactivator promotes transcript 3'-end cleavage.
2002,
Pubmed Mili,
Evidence for reassociation of RNA-binding proteins after cell lysis: implications for the interpretation of immunoprecipitation analyses.
2004,
Pubmed Millevoi,
A novel function for the U2AF 65 splicing factor in promoting pre-mRNA 3'-end processing.
2002,
Pubmed Millevoi,
An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries.
2006,
Pubmed Nesic,
A role for Cajal bodies in the final steps of U2 snRNP biogenesis.
2004,
Pubmed Neugebauer,
On the importance of being co-transcriptional.
2002,
Pubmed Patel,
Splicing double: insights from the second spliceosome.
2003,
Pubmed Proudfoot,
Integrating mRNA processing with transcription.
2002,
Pubmed Ryan,
Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease.
2004,
Pubmed Schmidt-Zachmann,
Molecular characterization of a novel, widespread nuclear protein that colocalizes with spliceosome components.
1998,
Pubmed
,
Xenbase Seghezzi,
Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells.
1998,
Pubmed Skolnik-David,
Electrophoretic separation of polyadenylation-specific complexes.
1987,
Pubmed Takagaki,
Complex protein interactions within the human polyadenylation machinery identify a novel component.
2000,
Pubmed Tanackovic,
Human splicing factor SF3a, but not SF1, is essential for pre-mRNA splicing in vivo.
2005,
Pubmed Tsai,
Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2.
2005,
Pubmed Tycowski,
Guide RNAs with 5' caps and novel box C/D snoRNA-like domains for modification of snRNAs in metazoa.
2004,
Pubmed Wang,
Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis.
1998,
Pubmed White,
Developmental and cell cycle regulation of the Drosophila histone locus body.
2007,
Pubmed Will,
A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.
2001,
Pubmed Will,
Identification of both shared and distinct proteins in the major and minor spliceosomes.
1999,
Pubmed Will,
Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.
2002,
Pubmed Wilusz,
A 64 kd nuclear protein binds to RNA segments that include the AAUAAA polyadenylation motif.
1988,
Pubmed Wu,
U7 small nuclear RNA in C snurposomes of the Xenopus germinal vesicle.
1993,
Pubmed
,
Xenbase Yu,
Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing.
1998,
Pubmed
,
Xenbase Zahler,
SR proteins: a conserved family of pre-mRNA splicing factors.
1992,
Pubmed Zheng,
Phosphorylation of stem-loop binding protein (SLBP) on two threonines triggers degradation of SLBP, the sole cell cycle-regulated factor required for regulation of histone mRNA processing, at the end of S phase.
2003,
Pubmed
,
Xenbase
