Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Physiol
2007 Dec 15;585Pt 3:867-79. doi: 10.1113/jphysiol.2007.145649.
Show Gene links
Show Anatomy links
TRESK two-pore-domain K+ channels constitute a significant component of background potassium currents in murine dorsal root ganglion neurones.
Dobler T, Springauf A, Tovornik S, Weber M, Schmitt A, Sedlmeier R, Wischmeyer E, Döring F.
???displayArticle.abstract??? TRESK (TWIK-related spinal cord K(+) channel) is the most recently identified member of the two-pore-domain potassium channel (K(2P)) family, the molecular source of background potassium currents. Human TRESK channels are not affected by external acidification. However, the mouse orthologue displays moderate pH dependence isolated to a single histidine residue adjacent to the GYG selectivity filter. In the human protein, sequence substitution of tyrosine by histidine at this critical position generated a mutant that displays almost identical proton sensitivity compared with mouse TRESK. In contrast to human TRESK, which is specifically located in spinal cord, we detected mouse TRESK (mTRESK) mRNA in several epithelial and neuronal tissues including lung, liver, kidney, brain and spinal cord. As revealed by endpoint and quantitative RT-PCR, mTRESK channels are mainly expressed in dorsal root ganglia (DRG) and on the transcript level represent the most important background potassium channel in this tissue. DRG neurones of all sizes were labelled by in situ hybridizations with TRESK-specific probes. In DRG neurones of TRESK[G339R] functional knock-out (KO) mice the standing outward current IK(so) was significantly reduced compared with TRESK wild-type (WT) littermates. Different responses to K(2P) channel regulators such as bupivacaine, extracellular protons and quinidine corroborated the finding that approximately 20% of IK(so) is carried by TRESK channels. Unexpectedly, we found no difference in resting membrane potential between DRG neurones of TRESK[WT] and TRESK[G339R] functional KO mice. However, in current-clamp recordings we observed significant changes in action potential duration and amplitude of after-hyperpolarization. Most strikingly, cellular excitability of DRG neurones from functional KO mice was significantly augmented as revealed by reduced rheobase current to elicit action potentials.
Augustin,
Efficient and fast targeted production of murine models based on ENU mutagenesis.
2005, Pubmed
Augustin,
Efficient and fast targeted production of murine models based on ENU mutagenesis.
2005,
Pubmed Bartsch,
Expression of tenascin in the developing and adult cerebellar cortex.
1992,
Pubmed Baumann,
Background potassium channel block and TRPV1 activation contribute to proton depolarization of sensory neurons from humans with neuropathic pain.
2004,
Pubmed Baumann,
Responses of adult human dorsal root ganglion neurons in culture to capsaicin and low pH.
1996,
Pubmed Bayliss,
The TASK family: two-pore domain background K+ channels.
2003,
Pubmed Bevan,
Protons: small stimulants of capsaicin-sensitive sensory nerves.
1994,
Pubmed Brickley,
TASK-3 two-pore domain potassium channels enable sustained high-frequency firing in cerebellar granule neurons.
2007,
Pubmed Brickley,
Adaptive regulation of neuronal excitability by a voltage-independent potassium conductance.
2001,
Pubmed Caterina,
The capsaicin receptor: a heat-activated ion channel in the pain pathway.
1997,
Pubmed
,
Xenbase Cooper,
Characterization and function of TWIK-related acid sensing K+ channels in a rat nociceptive cell.
2004,
Pubmed Czirják,
Targeting of calcineurin to an NFAT-like docking site is required for the calcium-dependent activation of the background K+ channel, TRESK.
2006,
Pubmed
,
Xenbase Czirják,
The two-pore domain K+ channel, TRESK, is activated by the cytoplasmic calcium signal through calcineurin.
2004,
Pubmed
,
Xenbase Czirják,
Zinc and mercuric ions distinguish TRESK from the other two-pore-domain K+ channels.
2006,
Pubmed
,
Xenbase Duprat,
TASK, a human background K+ channel to sense external pH variations near physiological pH.
1997,
Pubmed
,
Xenbase Fink,
A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids.
1998,
Pubmed
,
Xenbase Fink,
Cloning, functional expression and brain localization of a novel unconventional outward rectifier K+ channel.
1996,
Pubmed
,
Xenbase Fioretti,
Histamine activates a background, arachidonic acid-sensitive K channel in embryonic chick dorsal root ganglion neurons.
2004,
Pubmed Franks,
The TREK K2P channels and their role in general anaesthesia and neuroprotection.
2004,
Pubmed Goldstein,
Potassium leak channels and the KCNK family of two-P-domain subunits.
2001,
Pubmed Grigg,
Differential expression of voltage-gated potassium channel genes in auditory nuclei of the mouse brainstem.
2000,
Pubmed Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed Honoré,
The neuronal background K2P channels: focus on TREK1.
2007,
Pubmed Kang,
Functional expression of TRESK-2, a new member of the tandem-pore K+ channel family.
2004,
Pubmed Kang,
TREK-2 (K2P10.1) and TRESK (K2P18.1) are major background K+ channels in dorsal root ganglion neurons.
2006,
Pubmed Keshavaprasad,
Species-specific differences in response to anesthetics and other modulators by the K2P channel TRESK.
2005,
Pubmed Kim,
TASK-3, a new member of the tandem pore K(+) channel family.
2000,
Pubmed Krishtal,
A receptor for protons in the nerve cell membrane.
1980,
Pubmed Leonoudakis,
An open rectifier potassium channel with two pore domains in tandem cloned from rat cerebellum.
1998,
Pubmed
,
Xenbase Li,
Integrated platform for detection of DNA sequence variants using capillary array electrophoresis.
2002,
Pubmed Liu,
Potent activation of the human tandem pore domain K channel TRESK with clinical concentrations of volatile anesthetics.
2004,
Pubmed
,
Xenbase Lopes,
Proton block and voltage gating are potassium-dependent in the cardiac leak channel Kcnk3.
2000,
Pubmed
,
Xenbase Maingret,
TREK-1 is a heat-activated background K(+) channel.
2000,
Pubmed
,
Xenbase Maingret,
The endocannabinoid anandamide is a direct and selective blocker of the background K(+) channel TASK-1.
2001,
Pubmed Maingret,
TRAAK is a mammalian neuronal mechano-gated K+ channel.
1999,
Pubmed Medhurst,
Distribution analysis of human two pore domain potassium channels in tissues of the central nervous system and periphery.
2001,
Pubmed Morton,
Determinants of pH sensing in the two-pore domain K(+) channels TASK-1 and -2.
2003,
Pubmed
,
Xenbase Niemeyer,
Neutralization of a single arginine residue gates open a two-pore domain, alkali-activated K+ channel.
2007,
Pubmed Patel,
Inhalational anesthetics activate two-pore-domain background K+ channels.
1999,
Pubmed Petersen,
Multiple capsaicin-evoked currents in isolated rat sensory neurons.
1996,
Pubmed Pluteanu,
Alpha(1)-adrenoceptor-mediated depolarization and beta-mediated hyperpolarization in cultured rat dorsal root ganglion neurones.
2002,
Pubmed Rajan,
TASK-3, a novel tandem pore domain acid-sensitive K+ channel. An extracellular histiding as pH sensor.
2000,
Pubmed
,
Xenbase Rajan,
THIK-1 and THIK-2, a novel subfamily of tandem pore domain K+ channels.
2001,
Pubmed
,
Xenbase Sano,
A novel two-pore domain K+ channel, TRESK, is localized in the spinal cord.
2003,
Pubmed Sirois,
The TASK-1 two-pore domain K+ channel is a molecular substrate for neuronal effects of inhalation anesthetics.
2000,
Pubmed Talley,
Cns distribution of members of the two-pore-domain (KCNK) potassium channel family.
2001,
Pubmed Vandesompele,
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.
2002,
Pubmed Waldmann,
H(+)-gated cation channels: neuronal acid sensors in the NaC/DEG family of ion channels.
1998,
Pubmed Wemmie,
Acid-sensing ion channels: advances, questions and therapeutic opportunities.
2006,
Pubmed Zanzouri,
Membrane potential-regulated transcription of the resting K+ conductance TASK-3 via the calcineurin pathway.
2006,
Pubmed Zhang,
Modulation of action potential firing by iberiotoxin and NS1619 in rat dorsal root ganglion neurons.
2003,
Pubmed
