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Cell cycle progression during oocyte maturation requires the strict temporal regulation of maternal mRNA translation. The intrinsic basis of this temporal control has not been fully elucidated but appears to involve distinct mRNA 3' UTR regulatory elements. In this study, we identify a novel translational control sequence (TCS) that exerts repression of target mRNAs in immature oocytes of the frog, Xenopus laevis, and can direct early cytoplasmic polyadenylation and translational activation during oocyte maturation. The TCS is functionally distinct from the previously characterized Musashi/polyadenylation response element (PRE) and the cytoplasmic polyadenylation element (CPE). We report that TCS elements exert translational repression in both the Wee1 mRNA 3' UTR and the pericentriolar material-1 (Pcm-1) mRNA 3' UTR in immature oocytes. During oocyte maturation, TCS function directs the early translational activation of the Pcm-1 mRNA. By contrast, we demonstrate that CPE sequences flanking the TCS elements in the Wee1 3' UTR suppress the ability of the TCS to direct early translational activation. Our results indicate that a functional hierarchy exists between these distinct 3' UTR regulatory elements to control the timing of maternal mRNA translational activation during oocyte maturation.
Balczon,
Arrest of cell cycle progression during first interphase in murine zygotes microinjected with anti-PCM-1 antibodies.
2002, Pubmed
Balczon,
Arrest of cell cycle progression during first interphase in murine zygotes microinjected with anti-PCM-1 antibodies.
2002,
Pubmed Balczon,
PCM-1, A 228-kD centrosome autoantigen with a distinct cell cycle distribution.
1994,
Pubmed Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997,
Pubmed
,
Xenbase Barkoff,
Translational control of cyclin B1 mRNA during meiotic maturation: coordinated repression and cytoplasmic polyadenylation.
2000,
Pubmed
,
Xenbase Charlesworth,
Musashi regulates the temporal order of mRNA translation during Xenopus oocyte maturation.
2006,
Pubmed
,
Xenbase Charlesworth,
The temporal control of Wee1 mRNA translation during Xenopus oocyte maturation is regulated by cytoplasmic polyadenylation elements within the 3'-untranslated region.
2000,
Pubmed
,
Xenbase Charlesworth,
A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation.
2002,
Pubmed
,
Xenbase Charlesworth,
Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus oocytes.
2004,
Pubmed
,
Xenbase Colegrove-Otero,
RNA-binding proteins in early development.
2005,
Pubmed Dammermann,
Assembly of centrosomal proteins and microtubule organization depends on PCM-1.
2002,
Pubmed
,
Xenbase de Moor,
Mechanisms of translational control by the 3' UTR in development and differentiation.
2005,
Pubmed de Moor,
The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Ferby,
A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes.
1999,
Pubmed
,
Xenbase Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase Freeman,
Meiotic induction by Xenopus cyclin B is accelerated by coexpression with mosXe.
1991,
Pubmed
,
Xenbase Gillian-Daniel,
Modifications of the 5' cap of mRNAs during Xenopus oocyte maturation: independence from changes in poly(A) length and impact on translation.
1998,
Pubmed
,
Xenbase Gross,
The critical role of the MAP kinase pathway in meiosis II in Xenopus oocytes is mediated by p90(Rsk).
2000,
Pubmed
,
Xenbase Heikinheimo,
The molecular mechanisms of oocyte maturation and early embryonic development are unveiling new insights into reproductive medicine.
1998,
Pubmed Howard,
The mitogen-activated protein kinase signaling pathway stimulates mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation.
1999,
Pubmed
,
Xenbase Imai,
The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA.
2001,
Pubmed Keady,
MAPK interacts with XGef and is required for CPEB activation during meiosis in Xenopus oocytes.
2007,
Pubmed
,
Xenbase Kuersten,
The power of the 3' UTR: translational control and development.
2003,
Pubmed MacNicol,
Raf-1 kinase is essential for early Xenopus development and mediates the induction of mesoderm by FGF.
1993,
Pubmed
,
Xenbase McGowan,
Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15.
1993,
Pubmed Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Mendez,
Translational control by CPEB: a means to the end.
2001,
Pubmed
,
Xenbase Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase Mignone,
UTRdb and UTRsite: a collection of sequences and regulatory motifs of the untranslated regions of eukaryotic mRNAs.
2005,
Pubmed Mueller,
Cell cycle regulation of a Xenopus Wee1-like kinase.
1995,
Pubmed
,
Xenbase Murakami,
Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos.
1998,
Pubmed
,
Xenbase Nakahata,
Involvement of Xenopus Pumilio in the translational regulation that is specific to cyclin B1 mRNA during oocyte maturation.
2003,
Pubmed
,
Xenbase Nakajo,
Absence of Wee1 ensures the meiotic cell cycle in Xenopus oocytes.
2000,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Padmanabhan,
Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.
2006,
Pubmed
,
Xenbase Parker,
Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase.
1992,
Pubmed Prasad,
Mos 3' UTR regulatory differences underlie species-specific temporal patterns of Mos mRNA cytoplasmic polyadenylation and translational recruitment during oocyte maturation.
2008,
Pubmed
,
Xenbase Rassa,
Spacing constraints on reinitiation of paramyxovirus transcription: the gene end U tract acts as a spacer to separate gene end from gene start sites.
2000,
Pubmed Richter,
Cytoplasmic polyadenylation in development and beyond.
1999,
Pubmed
,
Xenbase Roy,
Activation of p34cdc2 kinase by cyclin A.
1991,
Pubmed
,
Xenbase Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase Tung,
Translational unmasking of Emi2 directs cytostatic factor arrest in meiosis II.
2007,
Pubmed
,
Xenbase Wu,
Zygote arrest 1 (Zar1) is a novel maternal-effect gene critical for the oocyte-to-embryo transition.
2003,
Pubmed
