Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Glycolytic activation at the onset of contractions in isolated Xenopus laevis single myofibres.
Walsh B, Stary CM, Howlett RA, Kelley KM, Hogan MC.
???displayArticle.abstract???
Intracellular pH (pHi) was measured in isolated Xenopus laevis single myofibres at the onset of contractions, with and without glycolytic blockade, to investigate the time course of glycolytic activation. Single myofibres (n=8; CON) were incubated in 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoyxmethyl ester (10 microM; for fluorescence measurement of pHi) and stimulated for 15 s at 0.67 Hz in anoxia in the absence (control condition; CON) and presence of a glycolytic inhibitor (1 mM iodoacetic acid; IAA). Intracellular pHi and tension were continuously recorded, and the differences in pHi between conditions were used to estimate the activation time of glycolysis. An immediate and steady increase in pHi (initial alkalosis) at the onset of contractions was similar between CON and IAA trials for the first 9 s of the contractile bout. However, from six contractions (approximately 10 s) throughout the remainder of the bout, IAA demonstrated a continued rise in pHi, in contrast to a progressive decrease in pHi in CON (P<0.05). These results demonstrate, with high temporal resolution, that glycolysis is activated within six contractions (10 s at 0.67 Hz) in single Xenopus skeletal muscle fibres.
Adams,
Muscle buffer capacity estimated from pH changes during rest-to-work transitions.
1990, Pubmed
Adams,
Muscle buffer capacity estimated from pH changes during rest-to-work transitions.
1990,
Pubmed Brault,
Phosphocreatine content of freeze-clamped muscle: influence of creatine kinase inhibition.
2003,
Pubmed Chance,
Skeletal muscle energetics with PNMR: personal views and historic perspectives.
2006,
Pubmed Conley,
Activation of glycolysis in human muscle in vivo.
1997,
Pubmed Conley,
Glycolysis is independent of oxygenation state in stimulated human skeletal muscle in vivo.
1998,
Pubmed Connett,
Glycolytic regulation during an aerobic rest-to-work transition in dog gracilis muscle.
1987,
Pubmed Connett,
Cytosolic pH during a rest-to-work transition in red muscle: application of enzyme equilibria.
1987,
Pubmed Constantin-Teodosiu,
Anaerobic energy production in human skeletal muscle in intense contraction: a comparison of 31P magnetic resonance spectroscopy and biochemical techniques.
1997,
Pubmed Crowther,
Control of glycolysis in contracting skeletal muscle. I. Turning it on.
2002,
Pubmed Dahlstedt,
Is creatine kinase responsible for fatigue? Studies of isolated skeletal muscle deficient in creatine kinase.
2000,
Pubmed Edman,
Contractile properties of mouse single muscle fibers, a comparison with amphibian muscle fibers.
2005,
Pubmed Greenhaff,
The metabolic responses of human type I and II muscle fibres during maximal treadmill sprinting.
1994,
Pubmed Haseler,
Phosphocreatine hydrolysis during submaximal exercise: the effect of FIO2.
1998,
Pubmed Henriksson,
Redox state changes in human skeletal muscle after isometric contraction.
1986,
Pubmed Hogan,
Human muscle performance and PCr hydrolysis with varied inspired oxygen fractions: a 31P-MRS study.
1999,
Pubmed Hogan,
Phosphorescence quenching method for measurement of intracellular PO2 in isolated skeletal muscle fibers.
1999,
Pubmed
,
Xenbase Howlett,
Skeletal muscle metabolism during high-intensity sprint exercise is unaffected by dichloroacetate or acetate infusion.
1999,
Pubmed Howlett,
Dichloroacetate accelerates the fall in intracellular PO2 at onset of contractions in Xenopus single muscle fibers.
2003,
Pubmed
,
Xenbase Hultman,
Acid-base balance during exercise.
1980,
Pubmed Jacobs,
Lactate in human skeletal muscle after 10 and 30 s of supramaximal exercise.
1983,
Pubmed Kindig,
Effects of acute creatine kinase inhibition on metabolism and tension development in isolated single myocytes.
2005,
Pubmed
,
Xenbase Lännergren,
The effect of temperature and stimulation scheme on fatigue and recovery in Xenopus muscle fibres.
1988,
Pubmed
,
Xenbase Lindinger,
Applying physicochemical principles to skeletal muscle acid-base status.
2005,
Pubmed Nagesser,
ATP formation and ATP hydrolysis during fatiguing, intermittent stimulation of different types of single muscle fibres from Xenopus laevis.
1993,
Pubmed
,
Xenbase Nagesser,
Metabolic changes with fatigue in different types of single muscle fibres of Xenopus laevis.
1992,
Pubmed
,
Xenbase Robergs,
Biochemistry of exercise-induced metabolic acidosis.
2004,
Pubmed Roussel,
Metabolic determinants of the onset of acidosis in exercising human muscle: a 31P-MRS study.
2003,
Pubmed Sahlin,
Determination of muscle pH and glycolytic flux by magnetic resonance spectroscopy in contracting human skeletal muscle may have systematic errors.
2005,
Pubmed Sahlin,
Non-invasive measurements of O2 availability in human skeletal muscle with near-infrared spectroscopy.
1992,
Pubmed Stary,
Intracellular pH during sequential, fatiguing contractile periods in isolated single Xenopus skeletal muscle fibers.
2005,
Pubmed
,
Xenbase Stary,
Resistance to fatigue of individual Xenopus single skeletal muscle fibres is correlated with mitochondrial volume density.
2004,
Pubmed
,
Xenbase Sugi,
Nuclear magnetic resonance studies on the mechanism of regulation of glycogenolysis in contracting skeletal muscle.
1989,
Pubmed Sullivan,
Skeletal muscle pH assessed by biochemical and 31P-MRS methods during exercise and recovery in men.
1994,
Pubmed van der Laarse,
Maximum rate of oxygen consumption and quantitative histochemistry of succinate dehydrogenase in single muscle fibres of Xenopus laevis.
1989,
Pubmed
,
Xenbase Walsh,
Measurement of activation energy and oxidative phosphorylation onset kinetics in isolated muscle fibers in the absence of cross-bridge cycling.
2006,
Pubmed
,
Xenbase Westerblad,
Changes of intracellular pH due to repetitive stimulation of single fibres from mouse skeletal muscle.
1992,
Pubmed Westerblad,
The effect of intracellular pH on contractile function of intact, single fibres of mouse muscle declines with increasing temperature.
1997,
Pubmed Westerblad,
Reduced maximum shortening velocity in the absence of phosphocreatine observed in intact fibres of Xenopus skeletal muscle.
1995,
Pubmed
,
Xenbase Yamada,
31P-NMR study of the regulation of glycogenolysis in living skeletal muscle.
1987,
Pubmed Yamada,
31P NMR study of the regulation of glycogenolysis in iodoacetate-treated skeletal muscle.
1988,
Pubmed Yamada,
31P nuclear magnetic resonance studies on the glycogenolysis regulation in resting and contracting frog skeletal muscle.
1993,
Pubmed
