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J Biol Chem
2008 Aug 08;28332:22283-94. doi: 10.1074/jbc.M801663200.
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Protein kinase C modulates inactivation of Kv3.3 channels.
Desai R, Kronengold J, Mei J, Forman SA, Kaczmarek LK.
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Modulation of some Kv3 family potassium channels by protein kinase C (PKC) regulates their amplitude and kinetics and adjusts firing patterns of auditory neurons in response to stimulation. Nevertheless, little is known about the modulation of Kv3.3, a channel that is widely expressed throughout the nervous system and is the dominant Kv3 family member in auditory brainstem. We have cloned the cDNA for the Kv3.3 channel from mouse brain and have expressed it in a mammalian cell line and in Xenopus oocytes to characterize its biophysical properties and modulation by PKC. Kv3.3 currents activate at positive voltages and undergo inactivation with time constants of 150-250 ms. Activators of PKC increased current amplitude and removed inactivation of Kv3.3 currents, and a specific PKC pseudosubstrate inhibitor peptide prevented the effects of the activators. Elimination of the first 78 amino acids of the N terminus of Kv3.3 produced noninactivating currents suggesting that PKC modulates N-type inactivation, potentially by phosphorylation of sites in this region. To identify potential phosphorylation sites, we investigated the response of channels in which serines in this N-terminal domain were subjected to mutagenesis. Our results suggest that serines at positions 3 and 9 are potential PKC phosphorylation sites. Computer simulations of model neurons suggest that phosphorylation of Kv3.3 by PKC may allow neurons to maintain action potential height during stimulation at high frequencies, and may therefore contribute to stimulus-induced changes in the intrinsic excitability of neurons such as those of the auditory brainstem.
Akemann,
Interaction of Kv3 potassium channels and resurgent sodium current influences the rate of spontaneous firing of Purkinje neurons.
2006, Pubmed
Akemann,
Interaction of Kv3 potassium channels and resurgent sodium current influences the rate of spontaneous firing of Purkinje neurons.
2006,
Pubmed Brand,
Precise inhibition is essential for microsecond interaural time difference coding.
2002,
Pubmed Brownell,
Organization of the cat trapezoid body and the discharge characteristics of its fibers.
1975,
Pubmed Chang,
Distribution of Kv3.3 potassium channel subunits in distinct neuronal populations of mouse brain.
2007,
Pubmed Corpet,
Multiple sequence alignment with hierarchical clustering.
1988,
Pubmed Covarrubias,
Elimination of rapid potassium channel inactivation by phosphorylation of the inactivation gate.
1994,
Pubmed
,
Xenbase Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed Fernandez,
Inactivation of Kv3.3 potassium channels in heterologous expression systems.
2003,
Pubmed Forman,
Homologous mutations on different subunits cause unequal but additive effects on n-alcohol block in the nicotinic receptor pore.
1997,
Pubmed
,
Xenbase Ghanshani,
Genomic organization, nucleotide sequence, and cellular distribution of a Shaw-related potassium channel gene, Kv3.3, and mapping of Kv3.3 and Kv3.4 to human chromosomes 19 and 1.
1992,
Pubmed Goldman-Wohl,
Kv3.3b: a novel Shaw type potassium channel expressed in terminally differentiated cerebellar Purkinje cells and deep cerebellar nuclei.
1994,
Pubmed Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase House,
Protein kinase C contains a pseudosubstrate prototope in its regulatory domain.
1987,
Pubmed Kaczmarek,
Regulation of the timing of MNTB neurons by short-term and long-term modulation of potassium channels.
2005,
Pubmed Kanemasa,
Electrophysiological and pharmacological characterization of a mammalian Shaw channel expressed in NIH 3T3 fibroblasts.
1995,
Pubmed Kopp-Scheinpflug,
The medial nucleus of the trapezoid body in the gerbil is more than a relay: comparison of pre- and postsynaptic activity.
2003,
Pubmed Li,
Localization of two high-threshold potassium channel subunits in the rat central auditory system.
2001,
Pubmed Liu,
Depolarization selectively increases the expression of the Kv3.1 potassium channel in developing inferior colliculus neurons.
1998,
Pubmed Lorenzo,
The catalytic domain of protein kinase Cdelta confers protection from down-regulation induced by bryostatin 1.
1997,
Pubmed Macica,
Modulation of the kv3.1b potassium channel isoform adjusts the fidelity of the firing pattern of auditory neurons.
2003,
Pubmed Macica,
Casein kinase 2 determines the voltage dependence of the Kv3.1 channel in auditory neurons and transfected cells.
2001,
Pubmed Misonou,
Regulation of ion channel localization and phosphorylation by neuronal activity.
2004,
Pubmed Moreno,
Thalamocortical projections have a K+ channel that is phosphorylated and modulated by cAMP-dependent protein kinase.
1995,
Pubmed Perney,
Localization of a high threshold potassium channel in the rat cochlear nucleus.
1997,
Pubmed Rae,
Kv3.3 potassium channels in lens epithelium and corneal endothelium.
2000,
Pubmed Richardson,
Modification of delayed rectifier potassium currents by the Kv9.1 potassium channel subunit.
2000,
Pubmed
,
Xenbase Ron,
An autoregulatory region in protein kinase C: the pseudoanchoring site.
1995,
Pubmed
,
Xenbase Rudy,
Kv3 channels: voltage-gated K+ channels designed for high-frequency repetitive firing.
2001,
Pubmed Rudy,
Contributions of Kv3 channels to neuronal excitability.
1999,
Pubmed Smith,
Anatomy and physiology of principal cells of the medial nucleus of the trapezoid body (MNTB) of the cat.
1998,
Pubmed Song,
Acoustic environment determines phosphorylation state of the Kv3.1 potassium channel in auditory neurons.
2005,
Pubmed Strong,
Stimulation of protein kinase C recruits covert calcium channels in Aplysia bag cell neurons.
,
Pubmed Taschenberger,
Fine-tuning an auditory synapse for speed and fidelity: developmental changes in presynaptic waveform, EPSC kinetics, and synaptic plasticity.
2000,
Pubmed Wang,
Contribution of the Kv3.1 potassium channel to high-frequency firing in mouse auditory neurones.
1998,
Pubmed Wu,
Response of neurons in the lateral superior olive and medial nucleus of the trapezoid body to repetitive stimulation: intracellular and extracellular recordings from mouse brain slice.
1993,
Pubmed Zagotta,
Restoration of inactivation in mutants of Shaker potassium channels by a peptide derived from ShB.
1990,
Pubmed
,
Xenbase Zhou,
Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors.
2001,
Pubmed
,
Xenbase
