Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Autism is a complex disorder that arises from the pervasive action of genetic and epigenetic factors that alter synaptic connectivity of the brain. Although GABA and glutamate receptors seem to be two of those factors, very little is known about the functional properties of the autistic receptors. Autistic tissue samples stored in brain banks usually have relatively long postmortem times, and it is highly desirable to know whether neurotransmitter receptors in such tissues are still functional. Here we demonstrate that native receptors microtransplanted from autistic brains, as well as de novo mRNA-expressed receptors, are still functional and susceptible to detailed electrophysiological characterization even after long postmortem intervals. The opportunity to study the properties of human receptors present in diseased brains not only opens new avenues toward understanding autism and other neurological disorders, but it also makes the microtransplantation method a useful translational system to evaluate and develop novel medicinal drugs.
Atz,
Methodological considerations for gene expression profiling of human brain.
2007, Pubmed
Atz,
Methodological considerations for gene expression profiling of human brain.
2007,
Pubmed Bailey,
A clinicopathological study of autism.
1998,
Pubmed Bauman,
Neuroanatomic observations of the brain in autism: a review and future directions.
2005,
Pubmed Belmonte,
Autism as a disorder of neural information processing: directions for research and targets for therapy.
2004,
Pubmed Bernareggi,
Properties of glutamate receptors of Alzheimer's disease brain transplanted to frog oocytes.
2007,
Pubmed Blatt,
Density and distribution of hippocampal neurotransmitter receptors in autism: an autoradiographic study.
2001,
Pubmed Chao,
MeCP2 controls excitatory synaptic strength by regulating glutamatergic synapse number.
2007,
Pubmed Courchesne,
Brainstem, cerebellar and limbic neuroanatomical abnormalities in autism.
1997,
Pubmed Cummings,
Recovery and expression of messenger RNA from postmortem human brain tissue.
2001,
Pubmed Fatemi,
Reelin signaling is impaired in autism.
2005,
Pubmed Fucile,
Effects of cyclothiazide on GluR1/AMPA receptors.
2006,
Pubmed Gundersen,
Glutamate and kainate receptors induced by rat brain messenger RNA in Xenopus oocytes.
1984,
Pubmed
,
Xenbase Gundersen,
Serotonin receptors induced by exogenous messenger RNA in Xenopus oocytes.
1983,
Pubmed
,
Xenbase Guptill,
[3H]-flunitrazepam-labeled benzodiazepine binding sites in the hippocampal formation in autism: a multiple concentration autoradiographic study.
2007,
Pubmed Hogart,
15q11-13 GABAA receptor genes are normally biallelically expressed in brain yet are subject to epigenetic dysregulation in autism-spectrum disorders.
2007,
Pubmed Hynd,
Biochemical and molecular studies using human autopsy brain tissue.
2003,
Pubmed Jiang,
A mixed epigenetic/genetic model for oligogenic inheritance of autism with a limited role for UBE3A.
2004,
Pubmed Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase Ma,
Identification of significant association and gene-gene interaction of GABA receptor subunit genes in autism.
2005,
Pubmed Marsal,
Incorporation of acetylcholine receptors and Cl- channels in Xenopus oocytes injected with Torpedo electroplaque membranes.
1995,
Pubmed
,
Xenbase Martínez-Martínez,
Functional expression in frog oocytes of human rho 1 receptors produced in Saccharomyces cerevisiae.
2004,
Pubmed
,
Xenbase Mexal,
Brain pH has a significant impact on human postmortem hippocampal gene expression profiles.
2006,
Pubmed Miledi,
Microtransplantation of neurotransmitter receptors from cells to Xenopus oocyte membranes: new procedure for ion channel studies.
2006,
Pubmed
,
Xenbase Miledi,
Expression of functional neurotransmitter receptors in Xenopus oocytes after injection of human brain membranes.
2002,
Pubmed
,
Xenbase Miledi,
Microtransplantation of functional receptors and channels from the Alzheimer's brain to frog oocytes.
2004,
Pubmed
,
Xenbase Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase Nguyen,
A Windows software package to record from voltage-clamped Xenopus oocytes.
1995,
Pubmed
,
Xenbase Palma,
GABA(A)-current rundown of temporal lobe epilepsy is associated with repetitive activation of GABA(A) "phasic" receptors.
2007,
Pubmed
,
Xenbase Palma,
Expression of human epileptic temporal lobe neurotransmitter receptors in Xenopus oocytes: An innovative approach to study epilepsy.
2002,
Pubmed
,
Xenbase Palma,
Phosphatase inhibitors remove the run-down of gamma-aminobutyric acid type A receptors in the human epileptic brain.
2004,
Pubmed
,
Xenbase Parker,
Activation of a common effector system by different brain neurotransmitter receptors in Xenopus oocytes.
1987,
Pubmed
,
Xenbase Persico,
Searching for ways out of the autism maze: genetic, epigenetic and environmental clues.
2006,
Pubmed Pickles,
Latent-class analysis of recurrence risks for complex phenotypes with selection and measurement error: a twin and family history study of autism.
1995,
Pubmed Purcell,
Postmortem brain abnormalities of the glutamate neurotransmitter system in autism.
2001,
Pubmed Ragozzino,
Rundown of GABA type A receptors is a dysfunction associated with human drug-resistant mesial temporal lobe epilepsy.
2005,
Pubmed
,
Xenbase Ragsdale,
Expressional potency of mRNAs encoding receptors and voltage-activated channels in the postmortem rat brain.
1991,
Pubmed
,
Xenbase Samaco,
Epigenetic overlap in autism-spectrum neurodevelopmental disorders: MECP2 deficiency causes reduced expression of UBE3A and GABRB3.
2005,
Pubmed Spitzer,
A Rosetta stone for analysis of human membrane protein function.
2008,
Pubmed Zoghbi,
Postnatal neurodevelopmental disorders: meeting at the synapse?
2003,
Pubmed
