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Morpholino oligonucleotides are stable, uncharged, water-soluble molecules that bind to complementary sequences of RNA, thereby inhibiting mRNA processing, read-through, and protein binding at those sites. Morpholinos are typically used to inhibit translation of mRNA, splicing of pre-mRNA, and maturation of miRNA, although they can also inhibit other interactions between biological macromolecules and RNA. Morpholinos are effective, specific, and lack non-antisense effects. They work in any cell that transcribes and translates RNA. However, unmodified Morpholinos do not pass well through plasma membranes and must therefore be delivered into the nuclear or cytosolic compartment to be effective. Morpholinos form stable base pairs with complementary nucleic acid sequences but apparently do not bind to proteins to a significant extent. They are not recognized by proteins and do not undergo protein-mediated catalysis; nor do they mediate RNA cleavage by RNase H or the RISC complex. This work focuses on techniques and background for using Morpholinos.
Aboul-ela,
Base-base mismatches. Thermodynamics of double helix formation for dCA3XA3G + dCT3YT3G (X, Y = A,C,G,T).
1985, Pubmed
Aboul-ela,
Base-base mismatches. Thermodynamics of double helix formation for dCA3XA3G + dCT3YT3G (X, Y = A,C,G,T).
1985,
Pubmed Alonso,
Inhibition of infectious haematopoietic necrosis virus in cell cultures with peptide-conjugated morpholino oligomers.
2005,
Pubmed Bruno,
Correction of aberrant FGFR1 alternative RNA splicing through targeting of intronic regulatory elements.
2004,
Pubmed Cerda,
Electroporation of DNA, RNA, and morpholinos into zebrafish embryos.
2006,
Pubmed Cheong,
Solution structure of an unusually stable RNA tetraplex containing G- and U-quartet structures.
1992,
Pubmed Choi,
Target protectors reveal dampening and balancing of Nodal agonist and antagonist by miR-430.
2007,
Pubmed Deas,
Inhibition of flavivirus infections by antisense oligomers specifically suppressing viral translation and RNA replication.
2005,
Pubmed Draper,
Inhibition of zebrafish fgf8 pre-mRNA splicing with morpholino oligos: a quantifiable method for gene knockdown.
2001,
Pubmed
,
Xenbase Ekker,
Morphants: a new systematic vertebrate functional genomics approach.
2000,
Pubmed Ekker,
Morphant technology in model developmental systems.
2001,
Pubmed Enterlein,
VP35 knockdown inhibits Ebola virus amplification and protects against lethal infection in mice.
2006,
Pubmed Geller,
Antisense phosphorodiamidate morpholino oligomer inhibits viability of Escherichia coli in pure culture and in mouse peritonitis.
2005,
Pubmed Giles,
Antisense morpholino oligonucleotide analog induces missplicing of C-myc mRNA.
1999,
Pubmed Hayashi,
A sensitive and reproducible assay to measure the activity of glucosylceramide synthase and lactosylceramide synthase using HPLC and fluorescent substrates.
2005,
Pubmed He,
Amplification targeting: a modified pretargeting approach with potential for signal amplification-proof of a concept.
2004,
Pubmed He,
Pharmacokinetics in mice of four oligomer-conjugated polymers for amplification targeting.
2003,
Pubmed Heasman,
Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach.
2000,
Pubmed
,
Xenbase Henderson,
Reaction of diethyl pyrocarbonate with nucleic acid components. Adenosine-containing nucleotides and dinucleoside phosphates.
1973,
Pubmed Howard,
Efficient stimulation of site-specific ribosome frameshifting by antisense oligonucleotides.
2004,
Pubmed Hudziak,
Resistance of morpholino phosphorodiamidate oligomers to enzymatic degradation.
1996,
Pubmed Jubin,
Optimizing electroporation conditions for intracellular delivery of morpholino antisense oligonucleotides directed against the hepatitis C virus internal ribosome entry site.
2005,
Pubmed Jubin,
Hepatitis C virus internal ribosome entry site (IRES) stem loop IIId contains a phylogenetically conserved GGG triplet essential for translation and IRES folding.
2000,
Pubmed Kamachi,
Quantitative assessment of the knockdown efficiency of morpholino antisense oligonucleotides in zebrafish embryos using a luciferase assay.
2008,
Pubmed Kang,
Stacking interactions of ApA analogues with modified backbones.
1992,
Pubmed Khokha,
Techniques and probes for the study of Xenopus tropicalis development.
2002,
Pubmed
,
Xenbase Kimmel,
Cell lineage of zebrafish blastomeres. II. Formation of the yolk syncytial layer.
1985,
Pubmed Kimmel,
Cell lineage of zebrafish blastomeres. III. Clonal analyses of the blastula and gastrula stages.
1985,
Pubmed Kinney,
Inhibition of dengue virus serotypes 1 to 4 in vero cell cultures with morpholino oligomers.
2005,
Pubmed Kloosterman,
Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development.
2007,
Pubmed Kloosterman,
Substrate requirements for let-7 function in the developing zebrafish embryo.
2004,
Pubmed Kos,
Methods for introducing morpholinos into the chicken embryo.
2003,
Pubmed Lebedeva,
Antisense oligonucleotides: promise and reality.
2001,
Pubmed Li,
Design and synthesis of dendritic molecular transporter that achieves efficient in vivo delivery of morpholino antisense oligo.
2008,
Pubmed Liu,
The influence of chain length and base sequence on the pharmacokinetic behavior of 99mTc-morpholinos in mice.
2002,
Pubmed Liu,
Cytosine residues influence kidney accumulations of 99mTc-labeled morpholino oligomers.
2002,
Pubmed Mang'era,
Initial investigations of 99mTc-labeled morpholinos for radiopharmaceutical applications.
2001,
Pubmed Masaki,
Smad1 protects cardiomyocytes from ischemia-reperfusion injury.
2005,
Pubmed McCaffrey,
A potent and specific morpholino antisense inhibitor of hepatitis C translation in mice.
2003,
Pubmed McClorey,
Antisense oligonucleotide-induced exon skipping restores dystrophin expression in vitro in a canine model of DMD.
2006,
Pubmed Mellitzer,
Spatial and temporal 'knock down' of gene expression by electroporation of double-stranded RNA and morpholinos into early postimplantation mouse embryos.
2002,
Pubmed Melton,
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
2007,
Pubmed Monga,
Beta-catenin antisense studies in embryonic liver cultures: role in proliferation, apoptosis, and lineage specification.
2003,
Pubmed Morcos,
Achieving targeted and quantifiable alteration of mRNA splicing with Morpholino oligos.
2007,
Pubmed Morcos,
Achieving efficient delivery of morpholino oligos in cultured cells.
2001,
Pubmed Moulton,
Cellular uptake of antisense morpholino oligomers conjugated to arginine-rich peptides.
2004,
Pubmed Moulton,
Peptide-assisted delivery of steric-blocking antisense oligomers.
2003,
Pubmed Nasevicius,
Effective targeted gene 'knockdown' in zebrafish.
2000,
Pubmed Nelson,
Arginine-rich peptide conjugation to morpholino oligomers: effects on antisense activity and specificity.
2005,
Pubmed Neuman,
Antisense morpholino-oligomers directed against the 5' end of the genome inhibit coronavirus proliferation and growth.
2004,
Pubmed Neuman,
Inhibition, escape, and attenuated growth of severe acute respiratory syndrome coronavirus treated with antisense morpholino oligomers.
2005,
Pubmed Nutt,
Comparison of morpholino based translational inhibition during the development of Xenopus laevis and Xenopus tropicalis.
2001,
Pubmed
,
Xenbase Partridge,
A simple method for delivering morpholino antisense oligos into the cytoplasm of cells.
1996,
Pubmed Prasadan,
Glucagon is required for early insulin-positive differentiation in the developing mouse pancreas.
2002,
Pubmed Riedel-Kruse,
Synchrony dynamics during initiation, failure, and rescue of the segmentation clock.
2007,
Pubmed Sazani,
Systemically delivered antisense oligomers upregulate gene expression in mouse tissues.
2002,
Pubmed Sazani,
Nuclear antisense effects of neutral, anionic and cationic oligonucleotide analogs.
2001,
Pubmed Scacheri,
Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells.
2004,
Pubmed Schmajuk,
Antisense oligonucleotides with different backbones. Modification of splicing pathways and efficacy of uptake.
1999,
Pubmed Stancheva,
A mutant form of MeCP2 protein associated with human Rett syndrome cannot be displaced from methylated DNA by notch in Xenopus embryos.
2003,
Pubmed
,
Xenbase Stein,
A specificity comparison of four antisense types: morpholino, 2'-O-methyl RNA, DNA, and phosphorothioate DNA.
1997,
Pubmed Stein,
Inhibition of Vesivirus infections in mammalian tissue culture with antisense morpholino oligomers.
2001,
Pubmed Stirchak,
Uncharged stereoregular nucleic acid analogs: 2. Morpholino nucleoside oligomers with carbamate internucleoside linkages.
1989,
Pubmed Summerton,
Endo-Porter: a novel reagent for safe, effective delivery of substances into cells.
2005,
Pubmed Summerton,
Morpholino antisense oligomers: design, preparation, and properties.
1997,
Pubmed
,
Xenbase Summerton,
Morpholino antisense oligomers: the case for an RNase H-independent structural type.
1999,
Pubmed Suwanmanee,
Repair of a splicing defect in erythroid cells from patients with beta-thalassemia/HbE disorder.
2002,
Pubmed Takahashi,
Manipulating gene expressions by electroporation in the developing brain of mammalian embryos.
2002,
Pubmed Thummel,
Inhibition of zebrafish fin regeneration using in vivo electroporation of morpholinos against fgfr1 and msxb.
2006,
Pubmed Tidd,
Expression of c-myc is not critical for cell proliferation in established human leukemia lines.
2001,
Pubmed Tucker,
Antisense knockdown of the beta1 integrin subunit in the chicken embryo results in abnormal neural crest cell development.
2004,
Pubmed Tyson-Capper,
Novel targeting of cyclooxygenase-2 (COX-2) pre-mRNA using antisense morpholino oligonucleotides directed to the 3' acceptor and 5' donor splice sites of exon 4: suppression of COX-2 activity in human amnion-derived WISH and myometrial cells.
2006,
Pubmed van den Born,
Antiviral activity of morpholino oligomers designed to block various aspects of Equine arteritis virus amplification in cell culture.
2005,
Pubmed Warfield,
Gene-specific countermeasures against Ebola virus based on antisense phosphorodiamidate morpholino oligomers.
2006,
Pubmed Yen,
Exogenous control of mammalian gene expression through modulation of RNA self-cleavage.
2004,
Pubmed Youngblood,
Stability of cell-penetrating peptide-morpholino oligomer conjugates in human serum and in cells.
2007,
Pubmed
