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Neurosci Bull
2008 Oct 01;245:283-7. doi: 10.1007/s12264-008-0524-5.
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Deglycosylation altered the gating properties of rNav1.3: glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel.
Xu Q, Cheng HW, He HQ, Liu ZR, He M, Yang HT, Zhou ZL, Ji YH.
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To examine the effect of deglycosylation on gating properties of rNav1.3. rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control. Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV. Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.
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