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Am J Respir Cell Mol Biol
2009 May 01;405:543-54. doi: 10.1165/rcmb.2008-0166OC.
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Expression and regulation of epithelial Na+ channels by nucleotides in pleural mesothelial cells.
Nie HG, Tucker T, Su XF, Na T, Peng JB, Smith PR, Idell S, Ji HL.
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Pleural effusions are commonly clinical disorders, resulting from the imbalance between pleural fluid turnover and reabsorption. The mechanisms underlying pleural fluid clearance across the mesothelium remain to be elucidated. We hypothesized that epithelial Na(+) channel (ENaC) is expressed and forms the molecular basis of the amiloride-sensitive resistance in human mesothelial cells. Our RT-PCR results showed that three ENaC subunits, namely, alpha, beta, gamma, and two delta ENaC subunits, are expressed in human primary pleural mesothelial cells, a human mesothelioma cell line (M9K), and mouse pleural tissue. In addition, Western blotting and immunofluorescence microscopy studies revealed that alpha, beta, gamma, and delta ENaC subunits are expressed in primary human mesothelial cells and M9K cells at the protein level. An amiloride-inhibitable short-circuit current was detected in M9K monolayers and mouse pleural tissues when mounted in Ussing chambers. Whole-cell patch clamp recordings showed an ENaC-like channel with an amiloride concentration producing 50% inhibition of 12 microM in M9K cells. This cation channel has a high affinity for extracellular Na+ ions (K(m): 53 mM). The ion selectivity of this channel to cations follows the same order as ENaC: Li+ > Na+ > K+. The unitary Li(+) conductance was 15 pS in on-cell patches. Four ENaC subunits form a functional Na+ channel when coinjected into Xenopus oocytes. Furthermore, we found that both forskolin and cGMP increased the short-circuit currents in mouse pleural tissues. Taken together, our data demonstrate that the ENaC channels are biochemically and functionally expressed in human pleural mesothelial cells, and can be up-regulated by cyclic AMP and cyclic GMP.
Agostoni,
Solute-coupled liquid absorption from the pleural space.
1990, Pubmed
Agostoni,
Solute-coupled liquid absorption from the pleural space.
1990,
Pubmed Bao,
Ceramide mediates inhibition of the renal epithelial sodium channel by tumor necrosis factor-alpha through protein kinase C.
2007,
Pubmed
,
Xenbase Canessa,
Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits.
1994,
Pubmed
,
Xenbase Dvorak,
Tumors: wounds that do not heal. Similarities between tumor stroma generation and wound healing.
1986,
Pubmed Eaton,
Regulation of Na+ channels in lung alveolar type II epithelial cells.
2004,
Pubmed Fyfe,
Subunit composition determines the single channel kinetics of the epithelial sodium channel.
1998,
Pubmed
,
Xenbase Garty,
Characteristics and regulatory mechanisms of the amiloride-blockable Na+ channel.
1988,
Pubmed Guo,
Nitric oxide inhibits Na+ absorption across cultured alveolar type II monolayers.
1998,
Pubmed Idell,
Regulation of fibrin deposition by malignant mesothelioma.
1995,
Pubmed Inagaki,
Amiloride-sensitive epithelial Na+ channel currents in surface cells of rat rectal colon.
2004,
Pubmed Itani,
Glucocorticoid-stimulated lung epithelial Na(+) transport is associated with regulated ENaC and sgk1 expression.
2002,
Pubmed
,
Xenbase Ji,
Electrolyte and Fluid Transport in Mesothelial Cells.
2008,
Pubmed Ji,
The role of Pre-H2 domains of alpha- and delta-epithelial Na+ channels in ion permeation, conductance, and amiloride sensitivity.
2004,
Pubmed
,
Xenbase Ji,
Delta-subunit confers novel biophysical features to alpha beta gamma-human epithelial sodium channel (ENaC) via a physical interaction.
2006,
Pubmed Jiang,
WNK4 regulates the secretory pathway via which TRPV5 is targeted to the plasma membrane.
2008,
Pubmed
,
Xenbase Jiang,
Role of aquaporin and sodium channel in pleural water movement.
2003,
Pubmed Kashlan,
On the interaction between amiloride and its putative alpha-subunit epithelial Na+ channel binding site.
2005,
Pubmed
,
Xenbase Kellenberger,
Mutations in the epithelial Na+ channel ENaC outer pore disrupt amiloride block by increasing its dissociation rate.
2003,
Pubmed
,
Xenbase Kleyman,
The mechanism of action of amiloride.
1988,
Pubmed Kleyman,
Amiloride and its analogs as tools in the study of ion transport.
1988,
Pubmed Kunzelmann,
Culture-dependent expression of Na+ conductances in airway epithelial cells.
1996,
Pubmed Lai-Fook,
Pleural mechanics and fluid exchange.
2004,
Pubmed Lazrak,
Biophysical properties and molecular characterization of amiloride-sensitive sodium channels in A549 cells.
2000,
Pubmed Lazrak,
cAMP-induced changes of apical membrane potentials of confluent H441 monolayers.
2003,
Pubmed Matalon,
Biophysical and molecular properties of amiloride-inhibitable Na+ channels in alveolar epithelial cells.
1996,
Pubmed Matthay,
Alveolar epithelium: role in lung fluid balance and acute lung injury.
2005,
Pubmed Medford,
Pleural effusion.
2005,
Pubmed Miserocchi,
Physiology and pathophysiology of pleural fluid turnover.
1997,
Pubmed Mitrouska,
The trans-exudative pleural effusion.
2002,
Pubmed Mutsaers,
The mesothelial cell.
2004,
Pubmed Ramminger,
A regulated apical Na(+) conductance in dexamethasone-treated H441 airway epithelial cells.
2004,
Pubmed Russo,
Tumor necrosis factor enhances SN38-mediated apoptosis in mesothelioma cells: the role of nuclear factor-kappaB pathway activation.
2005,
Pubmed Sakuma,
Beta-adrenergic agonist stimulated alveolar fluid clearance in ex vivo human and rat lungs.
1997,
Pubmed Sartori,
Selected contribution: long-term effects of beta(2)-adrenergic receptor stimulation on alveolar fluid clearance in mice.
2002,
Pubmed Segal,
Influence of voltage and extracellular Na(+) on amiloride block and transport kinetics of rat epithelial Na(+) channel expressed in Xenopus oocytes.
2002,
Pubmed
,
Xenbase Shlyonsky,
Differentiation of epithelial Na+ channel function. An in vitro model.
2005,
Pubmed Stefanidis,
Amiloride-sensitive sodium channels on the parietal human peritoneum: evidence by ussing-type chamber experiments.
2007,
Pubmed Thome,
Modulation of sodium transport in fetal alveolar epithelial cells by oxygen and corticosterone.
2003,
Pubmed Voilley,
The lung amiloride-sensitive Na+ channel: biophysical properties, pharmacology, ontogenesis, and molecular cloning.
1994,
Pubmed Waldmann,
Molecular cloning and functional expression of a novel amiloride-sensitive Na+ channel.
1995,
Pubmed
,
Xenbase Wiener-Kronish,
Protein egress and entry rates in pleural fluid and plasma in sheep.
1984,
Pubmed Woollhead,
Phenformin and 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) activation of AMP-activated protein kinase inhibits transepithelial Na+ transport across H441 lung cells.
2005,
Pubmed Yue,
Culture-induced alterations in alveolar type II cell Na+ conductance.
1993,
Pubmed Yung,
Peritoneal mesothelial cell culture and biology.
2006,
Pubmed Zarogiannis,
Influence of the sodium transport inhibition by amiloride on the transmesothelial resistance of isolated visceral sheep peritoneum.
2005,
Pubmed Zarogiannis,
Comparison of the electrophysiological properties of the sheep isolated costal and diaphragmatic parietal pleura.
2007,
Pubmed Zocchi,
Electrolyte transport across the pleura of rabbits.
1991,
Pubmed Zocchi,
Physiology and pathophysiology of pleural fluid turnover.
2002,
Pubmed
