Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Physiol
2004 Jun 01;557Pt 2:347-54. doi: 10.1113/jphysiol.2003.059105.
Show Gene links
Show Anatomy links
Mapping the architecture of the ATP-binding site of the KATP channel subunit Kir6.2.
Dabrowski M, Tarasov A, Ashcroft FM.
???displayArticle.abstract???
ATP-sensitive potassium (K(ATP)) channels comprise Kir6.2 and SUR subunits. The site at which ATP binds to mediate K(ATP) channel inhibition lies on Kir6.2, but the potency of block is enhanced by coexpression with SUR1. To assess the structure of the ATP-binding site on Kir6.2, we used a range of adenine nucleotides as molecular measuring sticks to map the internal dimensions of the binding site. We compared their efficacy on Kir6.2-SUR1, and on a truncated Kir6.2 (Kir6.2DeltaC) that expresses in the absence of SUR. We show here that SUR1 modifies the ATP-binding pocket of Kir6.2, by increasing the width of the groove that binds the phosphate tail of ATP, without changing the length of the groove, and by enhancing interaction with the adenine ring.
Aguilar-Bryan,
Cloning of the beta cell high-affinity sulfonylurea receptor: a regulator of insulin secretion.
1995, Pubmed
Aguilar-Bryan,
Cloning of the beta cell high-affinity sulfonylurea receptor: a regulator of insulin secretion.
1995,
Pubmed Ammälä,
Inhibition of ATP-regulated K(+)-channels by a photoactivatable ATP-analogue in mouse pancreatic beta-cells.
1991,
Pubmed Ashcroft,
ATP-sensitive K+ channels in rat pancreatic beta-cells: modulation by ATP and Mg2+ ions.
1989,
Pubmed Ashcroft,
Adenosine 5'-triphosphate-sensitive potassium channels.
1988,
Pubmed Babenko,
SUR-dependent modulation of KATP channels by an N-terminal KIR6.2 peptide. Defining intersubunit gating interactions.
2002,
Pubmed Babenko,
The N-terminus of KIR6.2 limits spontaneous bursting and modulates the ATP-inhibition of KATP channels.
1999,
Pubmed Babenko,
A conserved inhibitory and differential stimulatory action of nucleotides on K(IR)6.0/SUR complexes is essential for excitation-metabolism coupling by K(ATP) channels.
2001,
Pubmed Gribble,
Properties of cloned ATP-sensitive K+ currents expressed in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Gribble,
MgATP activates the beta cell KATP channel by interaction with its SUR1 subunit.
1998,
Pubmed
,
Xenbase Inagaki,
Reconstitution of IKATP: an inward rectifier subunit plus the sulfonylurea receptor.
1995,
Pubmed Koster,
ATP inhibition of KATP channels: control of nucleotide sensitivity by the N-terminal domain of the Kir6.2 subunit.
1999,
Pubmed Martín,
Intracellular diadenosine polyphosphates: a novel second messenger in stimulus-secretion coupling.
1998,
Pubmed Matsuo,
Different binding properties and affinities for ATP and ADP among sulfonylurea receptor subtypes, SUR1, SUR2A, and SUR2B.
2000,
Pubmed Reimann,
Involvement of the n-terminus of Kir6.2 in coupling to the sulphonylurea receptor.
1999,
Pubmed
,
Xenbase Sakura,
Cloning and functional expression of the cDNA encoding a novel ATP-sensitive potassium channel subunit expressed in pancreatic beta-cells, brain, heart and skeletal muscle.
1995,
Pubmed Seino,
Physiological and pathophysiological roles of ATP-sensitive K+ channels.
2003,
Pubmed Seino,
ATP-sensitive potassium channels: a model of heteromultimeric potassium channel/receptor assemblies.
1999,
Pubmed Shyng,
Regulation of KATP channel activity by diazoxide and MgADP. Distinct functions of the two nucleotide binding folds of the sulfonylurea receptor.
1997,
Pubmed Spruce,
Studies of the unitary properties of adenosine-5'-triphosphate-regulated potassium channels of frog skeletal muscle.
1987,
Pubmed Tanabe,
Direct photoaffinity labeling of Kir6.2 by [gamma-(32)P]ATP-[gamma]4-azidoanilide.
2000,
Pubmed
,
Xenbase Tanabe,
Direct photoaffinity labeling of the Kir6.2 subunit of the ATP-sensitive K+ channel by 8-azido-ATP.
1999,
Pubmed
,
Xenbase Trapp,
Identification of residues contributing to the ATP binding site of Kir6.2.
2003,
Pubmed
,
Xenbase Tucker,
Molecular determinants of KATP channel inhibition by ATP.
1998,
Pubmed
,
Xenbase Tucker,
Truncation of Kir6.2 produces ATP-sensitive K+ channels in the absence of the sulphonylurea receptor.
1997,
Pubmed
,
Xenbase Wang,
Compromised ATP binding as a mechanism of phosphoinositide modulation of ATP-sensitive K+ channels.
2002,
Pubmed Zerangue,
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
1999,
Pubmed
,
Xenbase
