Methods Mol Biol 2008 Jan 01;469:381-400. doi: 10.1007/978-1-60327-469-2_24.

Transgenic reporter tools tracing endogenous canonical Wnt signaling in Xenopus.

???displayArticle.abstract???
Activation of the canonical Wnt pathway leads to the transcriptional activation of a particular subset of downstream Wnt target genes. To track this localized cellular output in a living organism, reporter constructs can be designed containing multimerized consensus lymphoid enhancer binding factor (LEF)-1/T cell factor (TCF) transcription factor binding sites, generally referred to as TCF optimal promoter (TOP) sites. In Xenopus, several Wnt-responsive reporter systems have been designed containing a number of these TOP sites that, in combination with a minimal promoter, drive the expression of a reporter gene. Following transgenic integration in Xenopus embryos, a Wnt reporter tool reveals the spatiotemporal delineation of endogenous Wnt pathway activities throughout development. Assumed to be a general readout of the Wnt pathway, such reporters can assist in elucidating unknown functional implications in developing Xenopus embryos.

???displayArticle.pubmedLink??? 19109721
???displayArticle.link??? Methods Mol Biol