Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2008 Dec 09;10549:19276-81. doi: 10.1073/pnas.0810187105.
Show Gene links
Show Anatomy links
Voltage-dependent K+ channel gating and voltage sensor toxin sensitivity depend on the mechanical state of the lipid membrane.
Schmidt D, MacKinnon R.
???displayArticle.abstract???
Voltage-dependent K(+) (Kv) channels underlie action potentials through gating conformational changes that are driven by membrane voltage. In this study of the paddle chimera Kv channel, we demonstrate that the rate of channel opening, the voltage dependence of the open probability, and the maximum achievable open probability depend on the lipid membrane environment. The activity of the voltage sensor toxin VsTx1, which interferes with voltage-dependent gating by partitioning into the membrane and binding to the channel, also depends on the membrane. Membrane environmental factors that influence channel function are divisible into two general categories: lipid compositional and mechanical state. The mechanical state can have a surprisingly large effect on the function of a voltage-dependent K(+) channel, including its pharmacological interaction with voltage sensor toxins. The dependence of VSTx1 activity on the mechanical state of the membrane leads us to hypothesize that voltage sensor toxins exert their effect by perturbing the interaction forces that exist between the channel and the membrane.
Alabi,
Portability of paddle motif function and pharmacology in voltage sensors.
2007, Pubmed,
Xenbase
Alabi,
Portability of paddle motif function and pharmacology in voltage sensors.
2007,
Pubmed
,
Xenbase Dai,
Membrane tension in swelling and shrinking molluscan neurons.
1998,
Pubmed Dowhan,
Molecular basis for membrane phospholipid diversity: why are there so many lipids?
1997,
Pubmed Hill,
Isolation and characterization of the Xenopus oocyte plasma membrane: a new method for studying activity of water and solute transporters.
2005,
Pubmed
,
Xenbase Hoshi,
Shaker potassium channel gating. I: Transitions near the open state.
1994,
Pubmed
,
Xenbase Jung,
Solution structure and lipid membrane partitioning of VSTx1, an inhibitor of the KvAP potassium channel.
2005,
Pubmed Kwok,
Thermoelasticity of large lecithin bilayer vesicles.
1981,
Pubmed Laitko,
Membrane stretch slows the concerted step prior to opening in a Kv channel.
2006,
Pubmed
,
Xenbase Lee,
A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom.
2004,
Pubmed Long,
Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment.
2007,
Pubmed Long,
Voltage sensor of Kv1.2: structural basis of electromechanical coupling.
2005,
Pubmed Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed Lundbaek,
Lipid bilayer-mediated regulation of ion channel function by amphiphilic drugs.
2008,
Pubmed Marheineke,
Lipid composition of Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) insect cells used for baculovirus infection.
1998,
Pubmed Milescu,
Tarantula toxins interact with voltage sensors within lipid membranes.
2007,
Pubmed
,
Xenbase Morales,
Incorporation of reconstituted acetylcholine receptors from Torpedo into the Xenopus oocyte membrane.
1995,
Pubmed
,
Xenbase Oliver,
Functional conversion between A-type and delayed rectifier K+ channels by membrane lipids.
2004,
Pubmed
,
Xenbase Opsahl,
Lipid-glass adhesion in giga-sealed patch-clamped membranes.
1994,
Pubmed Perozo,
Physical principles underlying the transduction of bilayer deformation forces during mechanosensitive channel gating.
2002,
Pubmed Ramu,
Enzymatic activation of voltage-gated potassium channels.
2006,
Pubmed Rawicz,
Effect of chain length and unsaturation on elasticity of lipid bilayers.
2000,
Pubmed Renkonen,
The lipids of the plasma membranes and endoplasmic reticulum from cultured baby hamster kidney cells (BHK21).
1972,
Pubmed Robinson,
Functional binding of cardiolipin to cytochrome c oxidase.
1993,
Pubmed Ruta,
Functional analysis of an archaebacterial voltage-dependent K+ channel.
2003,
Pubmed Schmidt,
Phospholipids and the origin of cationic gating charges in voltage sensors.
2006,
Pubmed Schoppa,
Activation of Shaker potassium channels. III. An activation gating model for wild-type and V2 mutant channels.
1998,
Pubmed
,
Xenbase Shcherbatko,
Voltage-dependent sodium channel function is regulated through membrane mechanics.
1999,
Pubmed
,
Xenbase Sigworth,
The variance of sodium current fluctuations at the node of Ranvier.
1980,
Pubmed Suchyna,
Bilayer-dependent inhibition of mechanosensitive channels by neuroactive peptide enantiomers.
2004,
Pubmed Sukharev,
Energetic and spatial parameters for gating of the bacterial large conductance mechanosensitive channel, MscL.
1999,
Pubmed Swartz,
Mapping the receptor site for hanatoxin, a gating modifier of voltage-dependent K+ channels.
1997,
Pubmed Swartz,
Hanatoxin modifies the gating of a voltage-dependent K+ channel through multiple binding sites.
1997,
Pubmed
,
Xenbase Tabarean,
Membrane stretch affects gating modes of a skeletal muscle sodium channel.
1999,
Pubmed
,
Xenbase Tao,
Functional analysis of Kv1.2 and paddle chimera Kv channels in planar lipid bilayers.
2008,
Pubmed Valiyaveetil,
Lipids in the structure, folding, and function of the KcsA K+ channel.
2002,
Pubmed van Meer,
Membrane lipids: where they are and how they behave.
2008,
Pubmed Wolfe,
Mechanical properties of the plasma membrane of isolated plant protoplasts : mechanism of hyperosmotic and extracellular freezing injury.
1983,
Pubmed Yifrach,
Energetics of pore opening in a voltage-gated K(+) channel.
2002,
Pubmed
,
Xenbase
