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The frog Xenopus is a well established vertebrate model to study the processes involved in embryogenesis and organogenesis, as it can be manipulated easily with a whole series of methods. We have expanded these approaches by establishing two transgenic Xenopus strains that allow specific interference with the activity of defined genes using a heat-shock inducible Cre recombinase that can induce upon heat-shock expression of a reporter gene in crossings to a corresponding reporter strain. We have applied this binary technique of gene interference in Xenopus development to overexpress the mutated HNF1 beta transcription factor at distinct developmental stages. Induction of HNF1 beta P328L329del by heat-shock at the gastrula stage resulted in a dramatic phenotype including malformation of the pronephros, gut, stomach, abnormal tail development and massive edemas indicative for kidney dysfunction. Thus, we have established the first binary inducible gene expression system in Xenopus laevis that can be used to study organogenesis.
Beck,
Molecular pathways needed for regeneration of spinal cord and muscle in a vertebrate.
2003, Pubmed,
Xenbase
Beck,
Molecular pathways needed for regeneration of spinal cord and muscle in a vertebrate.
2003,
Pubmed
,
Xenbase Beck,
Gut specific expression using mammalian promoters in transgenic Xenopus laevis.
1999,
Pubmed
,
Xenbase Bingham,
Abnormal nephron development associated with a frameshift mutation in the transcription factor hepatocyte nuclear factor-1 beta.
2000,
Pubmed Bohn,
Distinct molecular and morphogenetic properties of mutations in the human HNF1beta gene that lead to defective kidney development.
2003,
Pubmed
,
Xenbase Branda,
Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice.
2004,
Pubmed Buchholz,
Transgenic analysis reveals that thyroid hormone receptor is sufficient to mediate the thyroid hormone signal in frog metamorphosis.
2004,
Pubmed
,
Xenbase Chae,
Inducible control of tissue-specific transgene expression in Xenopus tropicalis transgenic lines.
2002,
Pubmed
,
Xenbase Coffinier,
Bile system morphogenesis defects and liver dysfunction upon targeted deletion of HNF1beta.
2002,
Pubmed Das,
Controlling transgene expression to study Xenopus laevis metamorphosis.
2004,
Pubmed
,
Xenbase Edghill,
Mutations in hepatocyte nuclear factor-1beta and their related phenotypes.
2006,
Pubmed Fischer,
HNF1beta and defective nephrogenesis: a role for interacting partners?
2008,
Pubmed Fu,
Novel double promoter approach for identification of transgenic animals: A tool for in vivo analysis of gene function and development of gene-based therapies.
2002,
Pubmed
,
Xenbase Göttgens,
Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors.
2002,
Pubmed
,
Xenbase Gresh,
A transcriptional network in polycystic kidney disease.
2004,
Pubmed Halfon,
Targeted gene expression without a tissue-specific promoter: creating mosaic embryos using laser-induced single-cell heat shock.
1997,
Pubmed Halloran,
Laser-induced gene expression in specific cells of transgenic zebrafish.
2000,
Pubmed Hardy,
Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron.
2007,
Pubmed Hartley,
Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system.
2002,
Pubmed
,
Xenbase Haumaitre,
Lack of TCF2/vHNF1 in mice leads to pancreas agenesis.
2005,
Pubmed Hernandez,
vhnf1 integrates global RA patterning and local FGF signals to direct posterior hindbrain development in zebrafish.
2004,
Pubmed Hiesberger,
Mutation of hepatocyte nuclear factor-1beta inhibits Pkhd1 gene expression and produces renal cysts in mice.
2004,
Pubmed Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase Le,
Heat shock-inducible Cre/Lox approaches to induce diverse types of tumors and hyperplasia in transgenic zebrafish.
2007,
Pubmed Lecaudey,
Role of the hindbrain in patterning the otic vesicle: a study of the zebrafish vhnf1 mutant.
2007,
Pubmed Lin,
Requirement for Wnt and FGF signaling in Xenopus tadpole tail regeneration.
2008,
Pubmed
,
Xenbase Lokmane,
Crucial role of vHNF1 in vertebrate hepatic specification.
2008,
Pubmed Marsh-Armstrong,
Germ-line transmission of transgenes in Xenopus laevis.
1999,
Pubmed
,
Xenbase Michiue,
Temporal and spatial manipulation of gene expression in Xenopus embryos by injection of heat shock promoter-containing plasmids.
2005,
Pubmed
,
Xenbase Pan,
I-SceI meganuclease-mediated transgenesis in Xenopus.
2006,
Pubmed
,
Xenbase Ramos,
Temporal and spatial control of transgene expression using laser induction of the hsp70 promoter.
2006,
Pubmed Ridgway,
Tetracycline-regulated gene expression switch in Xenopus laevis.
2000,
Pubmed
,
Xenbase Ryffel,
Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus.
2000,
Pubmed
,
Xenbase Ryffel,
What can a frog tell us about human kidney development.
2003,
Pubmed
,
Xenbase Ryffel,
Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus.
2003,
Pubmed
,
Xenbase Shaner,
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
2004,
Pubmed Smith,
Targeted cell-ablation in Xenopus embryos using the conditional, toxic viral protein M2(H37A).
2007,
Pubmed
,
Xenbase Song,
Vhnf1 acts downstream of Bmp, Fgf, and RA signals to regulate endocrine beta cell development in zebrafish.
2007,
Pubmed Sun,
vhnf1, the MODY5 and familial GCKD-associated gene, regulates regional specification of the zebrafish gut, pronephros, and hindbrain.
2001,
Pubmed Thomas,
Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line.
2004,
Pubmed Thummel,
Cre-mediated site-specific recombination in zebrafish embryos.
2005,
Pubmed Waldner,
Transgenic Xenopus laevis strain expressing cre recombinase in muscle cells.
2006,
Pubmed
,
Xenbase Wang,
Selective deletion of the Hnf1beta (MODY5) gene in beta-cells leads to altered gene expression and defective insulin release.
2004,
Pubmed Werdien,
FLP and Cre recombinase function in Xenopus embryos.
2001,
Pubmed
,
Xenbase Wheeler,
Inducible gene expression in transgenic Xenopus embryos.
2000,
Pubmed
,
Xenbase Wiellette,
vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain.
2003,
Pubmed Wild,
The mutated human gene encoding hepatocyte nuclear factor 1beta inhibits kidney formation in developing Xenopus embryos.
2000,
Pubmed
,
Xenbase Wu,
The HNF1beta transcription factor has several domains involved in nephrogenesis and partially rescues Pax8/lim1-induced kidney malformations.
2004,
Pubmed
,
Xenbase
