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Proc Natl Acad Sci U S A
2009 Mar 31;10613:5181-6. doi: 10.1073/pnas.0812889106.
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An embryonic stem cell chromatin remodeling complex, esBAF, is essential for embryonic stem cell self-renewal and pluripotency.
Ho L, Ronan JL, Wu J, Staahl BT, Chen L, Kuo A, Lessard J, Nesvizhskii AI, Ranish J, Crabtree GR.
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Mammalian SWI/SNF [also called BAF (Brg/Brahma-associated factors)] ATP-dependent chromatin remodeling complexes are essential for formation of the totipotent and pluripotent cells of the early embryo. In addition, subunits of this complex have been recovered in screens for genes required for nuclear reprogramming in Xenopus and mouse embryonic stem cell (ES) morphology. However, the mechanism underlying the roles of these complexes is unclear. Here, we show that BAF complexes are required for the self-renewal and pluripotency of mouse ES cells but not for the proliferation of fibroblasts or other cells. Proteomic studies reveal that ES cells express distinctive complexes (esBAF) defined by the presence of Brg (Brahma-related gene), BAF155, and BAF60A, and the absence of Brm (Brahma), BAF170, and BAF60C. We show that this specialized subunit composition is required for ES cell maintenance and pluripotency. Our proteomic analysis also reveals that esBAF complexes interact directly with key regulators of pluripotency, suggesting that esBAF complexes are specialized to interact with ES cell-specific regulators, providing a potential explanation for the requirement of BAF complexes in pluripotency.
Battaglioli,
REST repression of neuronal genes requires components of the hSWI.SNF complex.
2002, Pubmed
Battaglioli,
REST repression of neuronal genes requires components of the hSWI.SNF complex.
2002,
Pubmed Bernstein,
A bivalent chromatin structure marks key developmental genes in embryonic stem cells.
2006,
Pubmed Biggar,
Continuous and widespread roles for the Swi-Snf complex in transcription.
1999,
Pubmed Boyer,
Polycomb complexes repress developmental regulators in murine embryonic stem cells.
2006,
Pubmed Boyer,
Core transcriptional regulatory circuitry in human embryonic stem cells.
2005,
Pubmed Breeden,
Cell cycle control of the yeast HO gene: cis- and trans-acting regulators.
1987,
Pubmed Bultman,
A Brg1 null mutation in the mouse reveals functional differences among mammalian SWI/SNF complexes.
2000,
Pubmed Chamberlain,
Polycomb repressive complex 2 is dispensable for maintenance of embryonic stem cell pluripotency.
2008,
Pubmed Chi,
Sequential roles of Brg, the ATPase subunit of BAF chromatin remodeling complexes, in thymocyte development.
2003,
Pubmed Chi,
Reciprocal regulation of CD4/CD8 expression by SWI/SNF-like BAF complexes.
2002,
Pubmed Choi,
Significance analysis of spectral count data in label-free shotgun proteomics.
2008,
Pubmed de la Serna,
Mammalian SWI/SNF complexes promote MyoD-mediated muscle differentiation.
2001,
Pubmed Fazzio,
An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity.
2008,
Pubmed Gao,
ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a.
2008,
Pubmed Han,
SRG3, a core component of mouse SWI/SNF complex, is essential for extra-embryonic vascular development.
2008,
Pubmed Hansis,
Nuclear reprogramming of human somatic cells by xenopus egg extract requires BRG1.
2004,
Pubmed
,
Xenbase Ho,
An embryonic stem cell chromatin remodeling complex, esBAF, is an essential component of the core pluripotency transcriptional network.
2009,
Pubmed Jiang,
A core Klf circuitry regulates self-renewal of embryonic stem cells.
2008,
Pubmed Khavari,
BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription.
1993,
Pubmed Kidder,
SWI/SNF-Brg1 regulates self-renewal and occupies core pluripotency-related genes in embryonic stem cells.
2009,
Pubmed Kim,
An extended transcriptional network for pluripotency of embryonic stem cells.
2008,
Pubmed Kim,
Srg3, a mouse homolog of yeast SWI3, is essential for early embryogenesis and involved in brain development.
2001,
Pubmed Klochendler-Yeivin,
The murine SNF5/INI1 chromatin remodeling factor is essential for embryonic development and tumor suppression.
2000,
Pubmed Lemon,
Selectivity of chromatin-remodelling cofactors for ligand-activated transcription.
,
Pubmed Lessard,
An essential switch in subunit composition of a chromatin remodeling complex during neural development.
2007,
Pubmed Lickert,
Baf60c is essential for function of BAF chromatin remodelling complexes in heart development.
2004,
Pubmed Loh,
The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells.
2006,
Pubmed Marson,
Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells.
2008,
Pubmed Martens,
Regulation of an intergenic transcript controls adjacent gene transcription in Saccharomyces cerevisiae.
2005,
Pubmed Masaki,
Developmental pluripotency-associated 4 (DPPA4) localized in active chromatin inhibits mouse embryonic stem cell differentiation into a primitive ectoderm lineage.
2007,
Pubmed Nesvizhskii,
Analysis and validation of proteomic data generated by tandem mass spectrometry.
2007,
Pubmed Nesvizhskii,
A statistical model for identifying proteins by tandem mass spectrometry.
2003,
Pubmed Olave,
Identification of a polymorphic, neuron-specific chromatin remodeling complex.
2002,
Pubmed Rando,
Rules and regulation in the primary structure of chromatin.
2007,
Pubmed Reyes,
Altered control of cellular proliferation in the absence of mammalian brahma (SNF2alpha).
1998,
Pubmed Surani,
Genetic and epigenetic regulators of pluripotency.
2007,
Pubmed Takahashi,
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
2006,
Pubmed Wang,
Diversity and specialization of mammalian SWI/SNF complexes.
1996,
Pubmed Wang,
Polybromo protein BAF180 functions in mammalian cardiac chamber maturation.
2004,
Pubmed Wang,
Purification and biochemical heterogeneity of the mammalian SWI-SNF complex.
1996,
Pubmed Wu,
Regulation of dendritic development by neuron-specific chromatin remodeling complexes.
2007,
Pubmed Yan,
BAF250B-associated SWI/SNF chromatin-remodeling complex is required to maintain undifferentiated mouse embryonic stem cells.
2008,
Pubmed Yan,
PBAF chromatin-remodeling complex requires a novel specificity subunit, BAF200, to regulate expression of selective interferon-responsive genes.
2005,
Pubmed Zhang,
Exit from G1 and S phase of the cell cycle is regulated by repressor complexes containing HDAC-Rb-hSWI/SNF and Rb-hSWI/SNF.
2000,
Pubmed Zybailov,
Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae.
2006,
Pubmed
