Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nature
2009 Jan 29;4577229:589-93. doi: 10.1038/nature07564.
Show Gene links
Show Anatomy links
WNT11 acts as a directional cue to organize the elongation of early muscle fibres.
Gros J, Serralbo O, Marcelle C.
???displayArticle.abstract???
The early vertebrate skeletal muscle is a well-organized tissue in which the primitive muscle fibres, the myocytes, are all parallel and aligned along the antero-posterior axis of the embryo. How myofibres acquire their orientation during development is unknown. Here we show that during early chick myogenesis WNT11 has an essential role in the oriented elongation of the myocytes. We find that the neural tube, known to drive WNT11 expression in the medial border of somites, is necessary and sufficient to orient myocyte elongation. We then show that the specific inhibition of WNT11 function in somites leads to the disorganization of myocytes. We establish that WNT11 mediates this effect through the evolutionary conserved planar cell polarity (PCP) pathway, downstream of the WNT/beta-catenin-dependent pathway, required to initiate the myogenic program of myocytes and WNT11 expression. Finally, we demonstrate that a localized ectopic source of WNT11 can markedly change the orientation of myocytes, indicating that WNT11 acts as a directional cue in this process. All together, these data show that the sequential action of the WNT/PCP and the WNT/beta-catenin pathways is necessary for the formation of fully functional embryonic muscle fibres. This study also provides evidence that WNTs can act as instructive cues to regulate the PCP pathway in vertebrates.
Anakwe,
16 Wnt regulation of limb muscle differentiation.
2002, Pubmed
Anakwe,
16 Wnt regulation of limb muscle differentiation.
2002,
Pubmed Axelrod,
Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways.
1998,
Pubmed
,
Xenbase Bastock,
Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning.
2003,
Pubmed Bhanot,
A new member of the frizzled family from Drosophila functions as a Wingless receptor.
1996,
Pubmed Borello,
The Wnt/beta-catenin pathway regulates Gli-mediated Myf5 expression during somitogenesis.
2006,
Pubmed Boutros,
Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling.
1998,
Pubmed Cooper,
Expression of avian prickle genes during early development and organogenesis.
2008,
Pubmed Das,
A robust system for RNA interference in the chicken using a modified microRNA operon.
2006,
Pubmed Djiane,
Role of frizzled 7 in the regulation of convergent extension movements during gastrulation in Xenopus laevis.
2000,
Pubmed
,
Xenbase Gros,
A two-step mechanism for myotome formation in chick.
2004,
Pubmed Gros,
A common somitic origin for embryonic muscle progenitors and satellite cells.
2005,
Pubmed Heisenberg,
Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation.
2000,
Pubmed Ikeya,
Wnt signaling from the dorsal neural tube is required for the formation of the medial dermomyotome.
1998,
Pubmed Jiang,
Ascidian prickle regulates both mediolateral and anterior-posterior cell polarity of notochord cells.
2005,
Pubmed Kahane,
Medial pioneer fibers pattern the morphogenesis of early myoblasts derived from the lateral somite.
2007,
Pubmed Kengaku,
Distinct WNT pathways regulating AER formation and dorsoventral polarity in the chick limb bud.
1998,
Pubmed Klein,
Planar cell polarization: an emerging model points in the right direction.
2005,
Pubmed Li,
Dishevelled proteins lead to two signaling pathways. Regulation of LEF-1 and c-Jun N-terminal kinase in mammalian cells.
1999,
Pubmed Linker,
Intrinsic signals regulate the initial steps of myogenesis in vertebrates.
2003,
Pubmed Linker,
beta-Catenin-dependent Wnt signalling controls the epithelial organisation of somites through the activation of paraxis.
2005,
Pubmed Marcelle,
Coordinate actions of BMPs, Wnts, Shh and noggin mediate patterning of the dorsal somite.
1997,
Pubmed Marlow,
Zebrafish Rho kinase 2 acts downstream of Wnt11 to mediate cell polarity and effective convergence and extension movements.
2002,
Pubmed
,
Xenbase Montross,
A beta-catenin/engrailed chimera selectively suppresses Wnt signaling.
2000,
Pubmed
,
Xenbase Morin,
Control of planar divisions by the G-protein regulator LGN maintains progenitors in the chick neuroepithelium.
2007,
Pubmed Park,
Subcellular localization and signaling properties of dishevelled in developing vertebrate embryos.
2005,
Pubmed
,
Xenbase Rothbächer,
Dishevelled phosphorylation, subcellular localization and multimerization regulate its role in early embryogenesis.
2000,
Pubmed
,
Xenbase Seifert,
Frizzled/PCP signalling: a conserved mechanism regulating cell polarity and directed motility.
2007,
Pubmed Tada,
Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway.
2000,
Pubmed
,
Xenbase Tao,
Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos.
2005,
Pubmed
,
Xenbase Torban,
Genetic interaction between members of the Vangl family causes neural tube defects in mice.
2008,
Pubmed Voiculescu,
The amniote primitive streak is defined by epithelial cell intercalation before gastrulation.
2007,
Pubmed
,
Xenbase Wallingford,
Dishevelled controls cell polarity during Xenopus gastrulation.
2000,
Pubmed
,
Xenbase Wang,
Tissue/planar cell polarity in vertebrates: new insights and new questions.
2007,
Pubmed Wang,
Dishevelled genes mediate a conserved mammalian PCP pathway to regulate convergent extension during neurulation.
2006,
Pubmed
,
Xenbase Witzel,
Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane.
2006,
Pubmed
