Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Slc26a9--anion exchanger, channel and Na+ transporter.
Chang MH, Plata C, Zandi-Nejad K, Sindić A, Sussman CR, Mercado A, Broumand V, Raghuram V, Mount DB, Romero MF.
???displayArticle.abstract???
The SLC26 gene family encodes anion transporters with diverse functional attributes: (a) anion exchanger, (b) anion sensor, and (c) anion conductance (likely channel). We have cloned and studied Slc26a9, a paralogue expressed mostly in lung and stomach. Immunohistochemistry shows that Slc26a9 is present at apical and intracellular membranes of lung and stomach epithelia. Using expression in Xenopus laevis oocytes and ion-sensitive microelectrodes, we discovered that Slc26a9 has a novel function not found in any other Slc26 proteins: cation coupling. Intracellular pH and voltage measurements show that Slc26a9 is a nCl(-)-HCO(3)(-) exchanger, suggesting roles in gastric HCl secretion or pulmonary HCO(3)(-) secretion; Na(+) electrodes and uptakes reveal that Slc26a9 has a cation dependence. Single-channel measurements indicate that Slc26a9 displays discrete open and closed states. These experiments show that Slc26a9 has three discrete physiological modes: nCl(-)-HCO(3)(-) exchanger, Cl(-) channel, and Na(+)-anion cotransporter. Thus, the Slc26a9 transporter channel is uniquely suited for dynamic and tissue-specific physiology or regulation in epithelial tissues.
Accardi,
Secondary active transport mediated by a prokaryotic homologue of ClC Cl- channels.
2004, Pubmed
Accardi,
Secondary active transport mediated by a prokaryotic homologue of ClC Cl- channels.
2004,
Pubmed Ahn,
Regulatory interaction between the cystic fibrosis transmembrane conductance regulator and HCO3- salvage mechanisms in model systems and the mouse pancreatic duct.
2001,
Pubmed Ballard,
CFTR involvement in chloride, bicarbonate, and liquid secretion by airway submucosal glands.
1999,
Pubmed Bissig,
Functional expression cloning of the canalicular sulfate transport system of rat hepatocytes.
1994,
Pubmed
,
Xenbase Bourbon,
Surfactant proteins in the digestive tract, mesentery, and other organs: evolutionary significance.
2001,
Pubmed Chernova,
Acute regulation of the SLC26A3 congenital chloride diarrhoea anion exchanger (DRA) expressed in Xenopus oocytes.
2003,
Pubmed
,
Xenbase Choi,
Aberrant CFTR-dependent HCO3- transport in mutations associated with cystic fibrosis.
2001,
Pubmed DeLaney,
Pulmonary mechanoreceptors in the dipnoi lungfish Protopterus and Lepidosiren.
1983,
Pubmed Dinour,
A novel missense mutation in the sodium bicarbonate cotransporter (NBCe1/SLC4A4) causes proximal tubular acidosis and glaucoma through ion transport defects.
2004,
Pubmed
,
Xenbase Dorwart,
SLC26A9 is a Cl(-) channel regulated by the WNK kinases.
2007,
Pubmed
,
Xenbase Everett,
A family of mammalian anion transporters and their involvement in human genetic diseases.
1999,
Pubmed Everett,
Pendred syndrome is caused by mutations in a putative sulphate transporter gene (PDS).
1997,
Pubmed Greeley,
Downregulated in adenoma and putative anion transporter are regulated by CFTR in cultured pancreatic duct cells.
2001,
Pubmed Grichtchenko,
Cloning, characterization, and chromosomal mapping of a human electroneutral Na(+)-driven Cl-HCO3 exchanger.
2001,
Pubmed
,
Xenbase Haila,
The congenital chloride diarrhea gene is expressed in seminal vesicle, sweat gland, inflammatory colon epithelium, and in some dysplastic colon cells.
2000,
Pubmed Haila,
SLC26A2 (diastrophic dysplasia sulfate transporter) is expressed in developing and mature cartilage but also in other tissues and cell types.
2001,
Pubmed Hästbacka,
Sulfate transport in chondrodysplasia.
1996,
Pubmed Hästbacka,
Linkage disequilibrium mapping in isolated founder populations: diastrophic dysplasia in Finland.
1992,
Pubmed Höglund,
Positional candidate genes for congenital chloride diarrhea suggested by high-resolution physical mapping in chromosome region 7q31.
1996,
Pubmed Ip,
Five tropical air-breathing fishes, six different strategies to defend against ammonia toxicity on land.
2004,
Pubmed Karniski,
Immunolocalization of sat-1 sulfate/oxalate/bicarbonate anion exchanger in the rat kidney.
1998,
Pubmed Kim,
SLC26A7 is a Cl- channel regulated by intracellular pH.
2005,
Pubmed
,
Xenbase Knauf,
Identification of a chloride-formate exchanger expressed on the brush border membrane of renal proximal tubule cells.
2001,
Pubmed
,
Xenbase Ko,
A molecular mechanism for aberrant CFTR-dependent HCO(3)(-) transport in cystic fibrosis.
2002,
Pubmed Ko,
Gating of CFTR by the STAS domain of SLC26 transporters.
2004,
Pubmed Kurita,
Identification of intestinal bicarbonate transporters involved in formation of carbonate precipitates to stimulate water absorption in marine teleost fish.
2008,
Pubmed
,
Xenbase Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase Lohi,
Mapping of five new putative anion transporter genes in human and characterization of SLC26A6, a candidate gene for pancreatic anion exchanger.
2000,
Pubmed Lohi,
Functional characterization of three novel tissue-specific anion exchangers SLC26A7, -A8, and -A9.
2002,
Pubmed
,
Xenbase Mansfield,
Multilocus linkage of familial hyperkalaemia and hypertension, pseudohypoaldosteronism type II, to chromosomes 1q31-42 and 17p11-q21.
1997,
Pubmed Moseley,
Downregulated in adenoma gene encodes a chloride transporter defective in congenital chloride diarrhea.
1999,
Pubmed
,
Xenbase Mount,
Isoforms of the Na-K-2Cl cotransporter in murine TAL I. Molecular characterization and intrarenal localization.
1999,
Pubmed
,
Xenbase Mount,
The SLC26 gene family of multifunctional anion exchangers.
2004,
Pubmed Petrovic,
Identification of a basolateral Cl-/HCO3- exchanger specific to gastric parietal cells.
2003,
Pubmed Picollo,
Chloride/proton antiporter activity of mammalian CLC proteins ClC-4 and ClC-5.
2005,
Pubmed Plata,
Zebrafish Slc5a12 encodes an electroneutral sodium monocarboxylate transporter (SMCTn). A comparison with the electrogenic SMCT (SMCTe/Slc5a8).
2007,
Pubmed
,
Xenbase Romero,
Cloning and functional expression of rNBC, an electrogenic Na(+)-HCO3- cotransporter from rat kidney.
1998,
Pubmed
,
Xenbase Romero,
Cloning and characterization of a Na+-driven anion exchanger (NDAE1). A new bicarbonate transporter.
2000,
Pubmed
,
Xenbase Romero,
Physiology of electrogenic SLC26 paralogues.
2006,
Pubmed
,
Xenbase Romero,
Expression cloning and characterization of a renal electrogenic Na+/HCO3- cotransporter.
1997,
Pubmed
,
Xenbase Satoh,
Functional analysis of diastrophic dysplasia sulfate transporter. Its involvement in growth regulation of chondrocytes mediated by sulfated proteoglycans.
1998,
Pubmed
,
Xenbase Scheel,
Voltage-dependent electrogenic chloride/proton exchange by endosomal CLC proteins.
2005,
Pubmed
,
Xenbase Schmitt,
Immunolocalization of the electrogenic Na+-HCO-3 cotransporter in mammalian and amphibian kidney.
1999,
Pubmed
,
Xenbase Schweinfest,
Identification of a colon mucosa gene that is down-regulated in colon adenomas and adenocarcinomas.
1993,
Pubmed Sciortino,
Cation and voltage dependence of rat kidney electrogenic Na(+)-HCO(-)(3) cotransporter, rkNBC, expressed in oocytes.
1999,
Pubmed
,
Xenbase Sciortino,
Localization of endogenous and recombinant Na(+)-driven anion exchanger protein NDAE1 from Drosophila melanogaster.
2001,
Pubmed
,
Xenbase Scott,
The Pendred syndrome gene encodes a chloride-iodide transport protein.
1999,
Pubmed
,
Xenbase Scott,
Human pendrin expressed in Xenopus laevis oocytes mediates chloride/formate exchange.
2000,
Pubmed
,
Xenbase Shcheynikov,
Coupling modes and stoichiometry of Cl-/HCO3- exchange by slc26a3 and slc26a6.
2006,
Pubmed
,
Xenbase Soleimani,
Pendrin: an apical Cl-/OH-/HCO3- exchanger in the kidney cortex.
2001,
Pubmed Wang,
Accessory protein facilitated CFTR-CFTR interaction, a molecular mechanism to potentiate the chloride channel activity.
2000,
Pubmed Wheat,
CFTR induces the expression of DRA along with Cl(-)/HCO(3)(-) exchange activity in tracheal epithelial cells.
2000,
Pubmed Xie,
Molecular characterization of the murine Slc26a6 anion exchanger: functional comparison with Slc26a1.
2002,
Pubmed
,
Xenbase Xu,
SLC26A9 is expressed in gastric surface epithelial cells, mediates Cl-/HCO3- exchange, and is inhibited by NH4+.
2005,
Pubmed Xu,
Deletion of the chloride transporter Slc26a9 causes loss of tubulovesicles in parietal cells and impairs acid secretion in the stomach.
2008,
Pubmed
,
Xenbase Zheng,
Prestin is the motor protein of cochlear outer hair cells.
2000,
Pubmed
