Exp Eye Res 2009 Jan 01;881:57-64. doi: 10.1016/j.exer.2008.09.022.

Ankyrin-B is required for coordinated expression of beta-2-spectrin, the Na/K-ATPase and the Na/Ca exchanger in the inner segment of rod photoreceptors.

???displayArticle.abstract???
Rod photoreceptors are highly polarized cells whose exquisite sensitivity to light depends on precise compartmentalization of ion channels/transporters within specialized membrane domains. Here, we report evidence for an ankyrin-B based mechanism for coordinated expression of the beta-2-spectrin-based membrane skeleton, and the Na/K-ATPase and Na/Ca exchanger in the inner segment of rod photoreceptors. We first discovered that ankyrin-B localizes to the inner segments but not outer segments of rod photoreceptors in vertebrates including humans, mice, and frogs. We found that haploinsufficiency of ankyrin-B in mice is accompanied by 50% reduction in inner segments of membrane proteins, including the Na/K-ATPase and the Na/Ca exchanger, as well as beta-2-spectrin, which is a component of the spectrin-actin membrane skeleton. These results are consistent with a mechanism where ankyrin-B is required to restrict the Na/K-ATPase and Na/Ca exchanger to the inner segment of rod photoreceptors by tethering these membrane proteins to beta-2-spectrin.

???displayArticle.pubmedLink??? 19007774
???displayArticle.link??? Exp Eye Res
???displayArticle.grants??? [+]

Species referenced: Xenopus
Genes referenced: actl6a ank1 ank2 atp1a1 dnai1 sptbn2
???displayArticle.antibodies??? Ank2 Ab1


???attribute.lit??? ???displayArticles.show???