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Mol Biol Cell
2009 Jun 01;2011:2684-96. doi: 10.1091/mbc.e08-10-1051.
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XMAP215-EB1 interaction is required for proper spindle assembly and chromosome segregation in Xenopus egg extract.
Kronja I, Kruljac-Letunic A, Caudron-Herger M, Bieling P, Karsenti E.
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In metaphase Xenopus egg extracts, global microtubule growth is mainly promoted by two unrelated microtubule stabilizers, end-binding protein 1 (EB1) and XMAP215. Here, we explore their role and potential redundancy in the regulation of spindle assembly and function. We find that at physiological expression levels, both proteins are required for proper spindle architecture: Spindles assembled in the absence of EB1 or at decreased XMAP215 levels are short and frequently multipolar. Moreover, the reduced density of microtubules at the equator of DeltaEB1 or DeltaXMAP215 spindles leads to faulty kinetochore-microtubule attachments. These spindles also display diminished poleward flux rates and, upon anaphase induction, they neither segregate chromosomes nor reorganize into interphasic microtubule arrays. However, EB1 and XMAP215 nonredundantly regulate spindle assembly because an excess of XMAP215 can compensate for the absence of EB1, whereas the overexpression of EB1 cannot substitute for reduced XMAP215 levels. Our data indicate that EB1 could positively regulate XMAP215 by promoting its binding to the microtubules. Finally, we show that disruption of the mitosis-specific XMAP215-EB1 interaction produces a phenotype similar to that of either EB1 or XMAP215 depletion. Therefore, the XMAP215-EB1 interaction is required for proper spindle organization and chromosome segregation in Xenopus egg extracts.
Akhmanova,
Tracking the ends: a dynamic protein network controls the fate of microtubule tips.
2008, Pubmed
Akhmanova,
Tracking the ends: a dynamic protein network controls the fate of microtubule tips.
2008,
Pubmed Bieling,
CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites.
2008,
Pubmed
,
Xenbase Bieling,
Reconstitution of a microtubule plus-end tracking system in vitro.
2007,
Pubmed Brouhard,
XMAP215 is a processive microtubule polymerase.
2008,
Pubmed
,
Xenbase Brown,
Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis.
1994,
Pubmed Burbank,
Slide-and-cluster models for spindle assembly.
2007,
Pubmed
,
Xenbase Buster,
Poleward tubulin flux in spindles: regulation and function in mitotic cells.
2007,
Pubmed Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase Carvalho,
Surfing on microtubule ends.
2003,
Pubmed Cassimeris,
TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly.
2004,
Pubmed Castoldi,
Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer.
2003,
Pubmed Caudron,
Spatial coordination of spindle assembly by chromosome-mediated signaling gradients.
2005,
Pubmed
,
Xenbase Chen,
Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores.
1996,
Pubmed
,
Xenbase Desai,
Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles.
1998,
Pubmed
,
Xenbase Duesbery,
CENP-E is an essential kinetochore motor in maturing oocytes and is masked during mos-dependent, cell cycle arrest at metaphase II.
1997,
Pubmed
,
Xenbase Garcia,
Fission yeast ch-TOG/XMAP215 homologue Alp14 connects mitotic spindles with the kinetochore and is a component of the Mad2-dependent spindle checkpoint.
2001,
Pubmed
,
Xenbase Goshima,
Length control of the metaphase spindle.
2005,
Pubmed Hannak,
Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts.
2006,
Pubmed
,
Xenbase Hannak,
Xorbit/CLASP links dynamic microtubules to chromosomes in the Xenopus meiotic spindle.
2006,
Pubmed
,
Xenbase Hayashi,
Structural basis for the activation of microtubule assembly by the EB1 and p150Glued complex.
2005,
Pubmed Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Howard,
Microtubule polymerases and depolymerases.
2007,
Pubmed
,
Xenbase Hyman,
Preparation of modified tubulins.
1991,
Pubmed Kapoor,
Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix.
2001,
Pubmed
,
Xenbase Karsenti,
The mitotic spindle: a self-made machine.
2001,
Pubmed
,
Xenbase Kinoshita,
Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis.
2005,
Pubmed
,
Xenbase Kwon,
Spindle mechanics and dynamics during mitosis in Drosophila.
2004,
Pubmed Lee,
MCAK associates with EB1.
2008,
Pubmed Maresca,
Methods for studying spindle assembly and chromosome condensation in Xenopus egg extracts.
2006,
Pubmed
,
Xenbase Matthews,
ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles.
1998,
Pubmed McNally,
Microtubule dynamics: new surprises from an old MAP.
2003,
Pubmed
,
Xenbase Mimori-Kiyosue,
The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules.
2000,
Pubmed
,
Xenbase Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase Mitchison,
Bipolarization and poleward flux correlate during Xenopus extract spindle assembly.
2004,
Pubmed
,
Xenbase Mitchison,
Mechanism and function of poleward flux in Xenopus extract meiotic spindles.
2005,
Pubmed
,
Xenbase Miyamoto,
The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles.
2004,
Pubmed
,
Xenbase Morgan,
Regulation of the APC and the exit from mitosis.
1999,
Pubmed Nakamura,
Critical role for the EB1 and APC interaction in the regulation of microtubule polymerization.
2001,
Pubmed Niethammer,
Discrete states of a protein interaction network govern interphase and mitotic microtubule dynamics.
2007,
Pubmed
,
Xenbase Ohi,
Nonredundant functions of Kinesin-13s during meiotic spindle assembly.
2007,
Pubmed
,
Xenbase Peset,
Function and regulation of Maskin, a TACC family protein, in microtubule growth during mitosis.
2005,
Pubmed
,
Xenbase Popov,
XMAP215 regulates microtubule dynamics through two distinct domains.
2001,
Pubmed
,
Xenbase Popov,
XMAP215 is required for the microtubule-nucleating activity of centrosomes.
2002,
Pubmed
,
Xenbase Ribbeck,
NuSAP, a mitotic RanGTP target that stabilizes and cross-links microtubules.
2006,
Pubmed
,
Xenbase Rieder,
Stuck in division or passing through: what happens when cells cannot satisfy the spindle assembly checkpoint.
2004,
Pubmed Rogers,
Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle.
2002,
Pubmed Sawin,
Poleward microtubule flux mitotic spindles assembled in vitro.
1991,
Pubmed
,
Xenbase Scholey,
Cell division.
2003,
Pubmed Severin,
Stu2 promotes mitotic spindle elongation in anaphase.
2001,
Pubmed Skop,
Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms.
2004,
Pubmed Slep,
Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1.
2007,
Pubmed Srayko,
Caenorhabditis elegans TAC-1 and ZYG-9 form a complex that is essential for long astral and spindle microtubules.
2003,
Pubmed Tirnauer,
EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules.
2002,
Pubmed
,
Xenbase Tournebize,
Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Vasquez,
Phosphorylation by CDK1 regulates XMAP215 function in vitro.
1999,
Pubmed
,
Xenbase Waterman-Storer,
Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells.
1998,
Pubmed
,
Xenbase Watson,
Microtubule plus-end loading of p150(Glued) is mediated by EB1 and CLIP-170 but is not required for intracellular membrane traffic in mammalian cells.
2006,
Pubmed Wilde,
Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran.
1999,
Pubmed
,
Xenbase Wittmann,
The spindle: a dynamic assembly of microtubules and motors.
2001,
Pubmed Zhang,
BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment.
2007,
Pubmed
,
Xenbase
