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Single cells and multicellular tissues rapidly heal wounds. These processes are considered distinct, but one mode of healing--Rho GTPase-dependent formation and closure of a purse string of actin filaments (F-actin) and myosin-2 around wounds--occurs in single cells and in epithelia. Here, we show that wounding of one cell in Xenopus embryos elicits Rho GTPase activation around the wound and at the nearest cell-cell junctions in the neighbor cells. F-actin and myosin-2 accumulate at the junctions and around the wound itself, and as the resultant actomyosin array closes over the wound site, junctional F-actin and myosin-2 become mechanically integrated with the actin and myosin-2 around the wound, forming a hybrid purse string. When cells are ablated rather than wounded, Rho GTPase activation and F-actin accumulation occur at cell-cell junctions surrounding the ablated cell, and the purse string closes the hole in the epithelium. Elevation of intracellular free calcium, an essential upstream signal for the single-cell wound response, also occurs at the cell-cell contacts and in neighbor cells. Thus, the single and multicellular purse string wound responses represent points on a signaling and mechanical continuum that are integrated by cell-cell junctions.
Bement,
Rehabilitation and the single cell.
2007, Pubmed
Bement,
Rehabilitation and the single cell.
2007,
Pubmed Bement,
A novel cytoskeletal structure involved in purse string wound closure and cell polarity maintenance.
1993,
Pubmed Bement,
Wound-induced assembly and closure of an actomyosin purse string in Xenopus oocytes.
1999,
Pubmed
,
Xenbase Benink,
Concentric zones of active RhoA and Cdc42 around single cell wounds.
2005,
Pubmed
,
Xenbase Braga,
The challenges of abundance: epithelial junctions and small GTPase signalling.
2005,
Pubmed Brock,
Healing of incisional wounds in the embryonic chick wing bud: characterization of the actin purse-string and demonstration of a requirement for Rho activation.
1996,
Pubmed Burkel,
Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin.
2007,
Pubmed
,
Xenbase Davidson,
Embryonic wound healing by apical contraction and ingression in Xenopus laevis.
2002,
Pubmed
,
Xenbase Florian,
Single-cell epithelial defects close rapidly by an actinomyosin purse string mechanism with functional tight junctions.
2002,
Pubmed Fukata,
Roles of Rho-family GTPases in cell polarisation and directional migration.
2003,
Pubmed Kofron,
Plakoglobin is required for maintenance of the cortical actin skeleton in early Xenopus embryos and for cdc42-mediated wound healing.
2002,
Pubmed
,
Xenbase Mandato,
Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte wounds.
2001,
Pubmed
,
Xenbase Martin,
Actin cables and epidermal movement in embryonic wound healing.
1992,
Pubmed McNeil,
Plasma membrane disruption: repair, prevention, adaptation.
2003,
Pubmed Minta,
Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores.
1989,
Pubmed Oancea,
Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals.
1998,
Pubmed Rosenblatt,
An epithelial cell destined for apoptosis signals its neighbors to extrude it by an actin- and myosin-dependent mechanism.
2001,
Pubmed Russo,
Distinct temporal-spatial roles for rho kinase and myosin light chain kinase in epithelial purse-string wound closure.
2005,
Pubmed Steinhardt,
Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release.
1994,
Pubmed Tamada,
Two distinct modes of myosin assembly and dynamics during epithelial wound closure.
2007,
Pubmed Terasaki,
Large plasma membrane disruptions are rapidly resealed by Ca2+-dependent vesicle-vesicle fusion events.
1997,
Pubmed Wood,
Wound healing recapitulates morphogenesis in Drosophila embryos.
2002,
Pubmed Yamada,
Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion.
2007,
Pubmed Yu,
Control of local actin assembly by membrane fusion-dependent compartment mixing.
2007,
Pubmed
,
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