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J Biol Chem
2009 Jun 26;28426:17700-10. doi: 10.1074/jbc.M109.008706.
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Proliferating cell nuclear antigen uses two distinct modes to move along DNA.
Kochaniak AB, Habuchi S, Loparo JJ, Chang DJ, Cimprich KA, Walter JC, van Oijen AM.
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Proliferating cell nuclear antigen (PCNA) plays an important role in eukaryotic genomic maintenance by topologically binding DNA and recruiting replication and repair proteins. The ring-shaped protein forms a closed circle around double-stranded DNA and is able to move along the DNA in a random walk. The molecular nature of this diffusion process is poorly understood. We use single-molecule imaging to visualize the movement of individual, fluorescently labeled PCNA molecules along stretched DNA. Measurements of diffusional properties as a function of viscosity and protein size suggest that PCNA moves along DNA using two different sliding modes. Most of the time, the clamp moves while rotationally tracking the helical pitch of the DNA duplex. In a less frequently used second mode of diffusion, the movement of the protein is uncoupled from the helical pitch, and the clamp diffuses at much higher rates.
Bagchi,
Diffusion constant of a nonspecifically bound protein undergoing curvilinear motion along DNA.
2008, Pubmed
Bagchi,
Diffusion constant of a nonspecifically bound protein undergoing curvilinear motion along DNA.
2008,
Pubmed Berg,
Diffusion-driven mechanisms of protein translocation on nucleic acids. 1. Models and theory.
1981,
Pubmed Blainey,
A base-excision DNA-repair protein finds intrahelical lesion bases by fast sliding in contact with DNA.
2006,
Pubmed Burgers,
ATP-independent loading of the proliferating cell nuclear antigen requires DNA ends.
1993,
Pubmed Cai,
Reconstitution of human replication factor C from its five subunits in baculovirus-infected insect cells.
1996,
Pubmed Chang,
Monoubiquitination of proliferating cell nuclear antigen induced by stalled replication requires uncoupling of DNA polymerase and mini-chromosome maintenance helicase activities.
2006,
Pubmed
,
Xenbase Ellison,
Biochemical characterization of DNA damage checkpoint complexes: clamp loader and clamp complexes with specificity for 5' recessed DNA.
2003,
Pubmed Fien,
Identification of replication factor C from Saccharomyces cerevisiae: a component of the leading-strand DNA replication complex.
1992,
Pubmed Fukuda,
Structure-function relationship of the eukaryotic DNA replication factor, proliferating cell nuclear antigen.
1995,
Pubmed Garg,
DNA polymerases that propagate the eukaryotic DNA replication fork.
2005,
Pubmed Gomes,
ATP utilization by yeast replication factor C. I. ATP-mediated interaction with DNA and with proliferating cell nuclear antigen.
2001,
Pubmed Gomes,
ATP utilization by yeast replication factor C. II. Multiple stepwise ATP binding events are required to load proliferating cell nuclear antigen onto primed DNA.
2001,
Pubmed Gorman,
Visualizing one-dimensional diffusion of proteins along DNA.
2008,
Pubmed Gorman,
Dynamic basis for one-dimensional DNA scanning by the mismatch repair complex Msh2-Msh6.
2007,
Pubmed Granéli,
Long-distance lateral diffusion of human Rad51 on double-stranded DNA.
2006,
Pubmed Gulbis,
Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA.
1996,
Pubmed Ha,
Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase.
2002,
Pubmed Harada,
Single-molecule imaging of RNA polymerase-DNA interactions in real time.
1999,
Pubmed Ivanov,
Proliferating cell nuclear antigen loaded onto double-stranded DNA: dynamics, minor groove interactions and functional implications.
2006,
Pubmed Kabata,
Visualization of single molecules of RNA polymerase sliding along DNA.
1993,
Pubmed Kim,
Single-molecule analysis of 1D diffusion and transcription elongation of T7 RNA polymerase along individual stretched DNA molecules.
2007,
Pubmed Komazin-Meredith,
Hopping of a processivity factor on DNA revealed by single-molecule assays of diffusion.
2008,
Pubmed Krishna,
Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA.
1994,
Pubmed Laurence,
Motion of a DNA sliding clamp observed by single molecule fluorescence spectroscopy.
2008,
Pubmed Lavalette,
Proteins as micro viscosimeters: Brownian motion revisited.
2006,
Pubmed Lavalette,
Microscopic viscosity and rotational diffusion of proteins in a macromolecular environment.
1999,
Pubmed Lee,
DNA primase acts as a molecular brake in DNA replication.
2006,
Pubmed Méchali,
DNA synthesis in a cell-free system from Xenopus eggs: priming and elongation on single-stranded DNA in vitro.
1982,
Pubmed
,
Xenbase Moldovan,
PCNA, the maestro of the replication fork.
2007,
Pubmed Ng,
Interaction of DNA polymerase delta, proliferating cell nuclear antigen, and synthetic oligonucleotide template-primers. Analysis by polyacrylamide gel electrophoresis-band mobility shift assay.
1993,
Pubmed Podust,
Replication factor C disengages from proliferating cell nuclear antigen (PCNA) upon sliding clamp formation, and PCNA itself tethers DNA polymerase delta to DNA.
1998,
Pubmed Podust,
DNA polymerase delta holoenzyme: action on single-stranded DNA and on double-stranded DNA in the presence of replicative DNA helicases.
1995,
Pubmed Podust,
Mammalian DNA polymerase auxiliary proteins: analysis of replication factor C-catalyzed proliferating cell nuclear antigen loading onto circular double-stranded DNA.
1995,
Pubmed Randell,
The herpes simplex virus processivity factor, UL42, binds DNA as a monomer.
2004,
Pubmed Schurr,
The one-dimensional diffusion coefficient of proteins absorbed on DNA. Hydrodynamic considerations.
1979,
Pubmed Schurtenberger,
The solution structure of functionally active human proliferating cell nuclear antigen determined by small-angle neutron scattering.
1998,
Pubmed Tafvizi,
Tumor suppressor p53 slides on DNA with low friction and high stability.
2008,
Pubmed Thompson,
Precise nanometer localization analysis for individual fluorescent probes.
2002,
Pubmed Tinker,
Detecting the ability of viral, bacterial and eukaryotic replication proteins to track along DNA.
1994,
Pubmed Tsurimoto,
Replication factors required for SV40 DNA replication in vitro. I. DNA structure-specific recognition of a primer-template junction by eukaryotic DNA polymerases and their accessory proteins.
1991,
Pubmed Uhlmann,
Deletion analysis of the large subunit p140 in human replication factor C reveals regions required for complex formation and replication activities.
1997,
Pubmed Walter,
Regulated chromosomal DNA replication in the absence of a nucleus.
1998,
Pubmed
,
Xenbase Wang,
Single molecule measurements of repressor protein 1D diffusion on DNA.
2006,
Pubmed Yao,
Clamp loading, unloading and intrinsic stability of the PCNA, beta and gp45 sliding clamps of human, E. coli and T4 replicases.
1996,
Pubmed Yildiz,
Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization.
2003,
Pubmed Zuccola,
The crystal structure of an unusual processivity factor, herpes simplex virus UL42, bound to the C terminus of its cognate polymerase.
2000,
Pubmed
