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Genes Dev
2009 Sep 15;2318:2224-36. doi: 10.1101/gad.1844309.
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Releasing cohesin from chromosome arms in early mitosis: opposing actions of Wapl-Pds5 and Sgo1.
Shintomi K, Hirano T.
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The cohesin complex establishes sister chromatid cohesion during S phase. In metazoan cells, most if not all cohesin dissociates from chromatin during mitotic prophase, leading to the formation of metaphase chromosomes with two cytologically discernible chromatids. This process, known as sister chromatid resolution, is believed to be a prerequisite for synchronous separation of sister chromatids in subsequent anaphase. To dissect this process at a mechanistic level, we set up an in vitro system. Sister chromatid resolution is severely impaired upon depletion of Wapl from Xenopus egg extracts. Exogenously added human Wapl can rescue these defects and, remarkably, it can do so in a very short time window of early mitosis. A similar set of observations is made for Pds5, a factor implicated previously in the stabilization of interphase cohesion. Characteristic amino acid motifs (the FGF motifs) in Wapl coordinate its physical and functional interactions with Pds5 and cohesin subunits. We propose that Wapl and Pds5 directly modulate conformational changes of cohesin to make it competent for dissociation from chromatin during prophase. Evidence is also presented that Sgo1 plays a hitherto underappreciated role in stabilizing cohesin along chromosome arms, which is antagonized by the mitotic kinases polo-like kinsase (Plk1) and aurora B.
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Anderson,
Condensin and cohesin display different arm conformations with characteristic hinge angles.
2002, Pubmed,
Xenbase
Anderson,
Condensin and cohesin display different arm conformations with characteristic hinge angles.
2002,
Pubmed
,
Xenbase Bayliss,
Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking.
2000,
Pubmed Belmont,
A three-dimensional approach to mitotic chromosome structure: evidence for a complex hierarchical organization.
1987,
Pubmed Bernard,
Cell-cycle regulation of cohesin stability along fission yeast chromosomes.
2008,
Pubmed Boyarchuk,
Bub1 is essential for assembly of the functional inner centromere.
2007,
Pubmed
,
Xenbase Dobie,
Identification of chromosome inheritance modifiers in Drosophila melanogaster.
2001,
Pubmed Gandhi,
Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase.
2006,
Pubmed Gruber,
Chromosomal cohesin forms a ring.
2003,
Pubmed Haering,
The cohesin ring concatenates sister DNA molecules.
2008,
Pubmed Haering,
Molecular architecture of SMC proteins and the yeast cohesin complex.
2002,
Pubmed Hartman,
Pds5p is an essential chromosomal protein required for both sister chromatid cohesion and condensation in Saccharomyces cerevisiae.
2000,
Pubmed Hauf,
Cohesin cleavage by separase required for anaphase and cytokinesis in human cells.
2001,
Pubmed Hirano,
Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts.
1993,
Pubmed
,
Xenbase Hirano,
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
1997,
Pubmed
,
Xenbase Kitajima,
Shugoshin collaborates with protein phosphatase 2A to protect cohesin.
2006,
Pubmed Kueng,
Wapl controls the dynamic association of cohesin with chromatin.
2006,
Pubmed Lee,
The molecular basis of sister-chromatid cohesion.
2001,
Pubmed Losada,
Identification of Xenopus SMC protein complexes required for sister chromatid cohesion.
1998,
Pubmed
,
Xenbase Losada,
Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis.
2002,
Pubmed
,
Xenbase Losada,
Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes.
2000,
Pubmed
,
Xenbase Losada,
Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts.
2005,
Pubmed
,
Xenbase Losada,
Dynamic molecular linkers of the genome: the first decade of SMC proteins.
2005,
Pubmed Murray,
Cell cycle extracts.
1991,
Pubmed Nakajima,
The complete removal of cohesin from chromosome arms depends on separase.
2007,
Pubmed Nasmyth,
The structure and function of SMC and kleisin complexes.
2005,
Pubmed Neuwald,
HEAT repeats associated with condensins, cohesins, and other complexes involved in chromosome-related functions.
2000,
Pubmed
,
Xenbase Onn,
Sister chromatid cohesion: a simple concept with a complex reality.
2008,
Pubmed Ono,
Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells.
2003,
Pubmed
,
Xenbase Panizza,
Pds5 cooperates with cohesin in maintaining sister chromatid cohesion.
,
Pubmed Peters,
The cohesin complex and its roles in chromosome biology.
2008,
Pubmed Rivera,
Shugoshin regulates cohesion by driving relocalization of PP2A in Xenopus extracts.
2009,
Pubmed
,
Xenbase Rolef Ben-Shahar,
Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion.
2008,
Pubmed Rowland,
Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity.
2009,
Pubmed Sumara,
The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase.
2002,
Pubmed
,
Xenbase Sutani,
Budding yeast Wpl1(Rad61)-Pds5 complex counteracts sister chromatid cohesion-establishing reaction.
2009,
Pubmed Tanaka,
Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism.
2001,
Pubmed Uhlmann,
Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1.
1999,
Pubmed Vernì,
Genetic and molecular analysis of wings apart-like (wapl), a gene controlling heterochromatin organization in Drosophila melanogaster.
2000,
Pubmed Waizenegger,
Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase.
2000,
Pubmed
,
Xenbase Woo,
Structural studies of a bacterial condensin complex reveal ATP-dependent disruption of intersubunit interactions.
2009,
Pubmed You,
ATM activation and its recruitment to damaged DNA require binding to the C terminus of Nbs1.
2005,
Pubmed
,
Xenbase Zhang,
Mice lacking sister chromatid cohesion protein PDS5B exhibit developmental abnormalities reminiscent of Cornelia de Lange syndrome.
2007,
Pubmed
