Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2009 Oct 23;28443:29847-59. doi: 10.1074/jbc.M109.044453.
Show Gene links
Show Anatomy links
Transmembrane protein-free membranes fuse into xenopus nuclear envelope and promote assembly of functional pores.
Rafikova ER, Melikov K, Ramos C, Dye L, Chernomordik LV.
???displayArticle.abstract???
Post-mitotic reassembly of nuclear envelope (NE) and the endoplasmic reticulum (ER) has been reconstituted in a cell-free system based on interphase Xenopus egg extract. To evaluate the relative contributions of cytosolic and transmembrane proteins in NE and ER assembly, we replaced a part of native membrane vesicles with ones either functionally impaired by trypsin or N-ethylmaleimide treatments or with protein-free liposomes. Although neither impaired membrane vesicles nor liposomes formed ER and nuclear membrane, they both supported assembly reactions by fusing with native membrane vesicles. At membrane concentrations insufficient to generate full-sized functional nuclei, addition of liposomes and their fusion with membrane vesicles resulted in an extensive expansion of NE, further chromatin decondensation, restoration of the functionality, and spatial distribution of the nuclear pore complexes (NPCs), and, absent newly delivered transmembrane proteins, an increase in NPC numbers. This rescue of the nuclear assembly by liposomes was inhibited by wheat germ agglutinin and thus required active nuclear transport, similarly to the assembly of full-sized functional NE with membrane vesicles. Mechanism of fusion between liposomes and between liposomes and membrane vesicles was investigated using lipid mixing assay. This fusion required interphase cytosol and, like fusion between native membrane vesicles, was inhibited by guanosine 5'-3-O-(thio)triphosphate, soluble N-ethylmaleimide-sensitive factor attachment protein, and N-ethylmaleimide. Our findings suggest that interphase cytosol contains proteins that mediate the fusion stage of ER and NE reassembly, emphasize an unexpected tolerance of nucleus assembly to changes in concentrations of transmembrane proteins, and reveal the existence of a feedback mechanism that couples NE expansion with NPC assembly.
Anderson,
The life cycle of the metazoan nuclear envelope.
2008, Pubmed
Anderson,
The life cycle of the metazoan nuclear envelope.
2008,
Pubmed Anderson,
Shaping the endoplasmic reticulum into the nuclear envelope.
2008,
Pubmed Anderson,
Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum.
2007,
Pubmed
,
Xenbase Antonin,
The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.
2005,
Pubmed
,
Xenbase Antonin,
Nuclear pore complex assembly through the cell cycle: regulation and membrane organization.
2008,
Pubmed Barszczewski,
A novel site of action for alpha-SNAP in the SNARE conformational cycle controlling membrane fusion.
2008,
Pubmed Baur,
NSF- and SNARE-mediated membrane fusion is required for nuclear envelope formation and completion of nuclear pore complex assembly in Xenopus laevis egg extracts.
2007,
Pubmed
,
Xenbase Boström,
SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity.
2007,
Pubmed Brandhorst,
Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity.
2006,
Pubmed Brügger,
Putative fusogenic activity of NSF is restricted to a lipid mixture whose coalescence is also triggered by other factors.
2000,
Pubmed Burger,
Greasing membrane fusion and fission machineries.
2000,
Pubmed Burke,
The laminopathies: the functional architecture of the nucleus and its contribution to disease.
2006,
Pubmed Byrne,
PLCgamma is enriched on poly-phosphoinositide-rich vesicles to control nuclear envelope assembly.
2007,
Pubmed Chernomordik,
Protein-lipid interplay in fusion and fission of biological membranes.
2003,
Pubmed D'Angelo,
Nuclear pores form de novo from both sides of the nuclear envelope.
2006,
Pubmed
,
Xenbase Davis,
Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway.
1987,
Pubmed Dreier,
In vitro formation of the endoplasmic reticulum occurs independently of microtubules by a controlled fusion reaction.
2000,
Pubmed
,
Xenbase Drummond,
Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types.
1999,
Pubmed
,
Xenbase Ellenberg,
Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis.
1997,
Pubmed Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
,
Xenbase FOLCH,
A simple method for the isolation and purification of total lipides from animal tissues.
1957,
Pubmed Franz,
Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates.
2005,
Pubmed
,
Xenbase Fukasawa,
Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation.
2004,
Pubmed Gant,
Roles of LAP2 proteins in nuclear assembly and DNA replication: truncated LAP2beta proteins alter lamina assembly, envelope formation, nuclear size, and DNA replication efficiency in Xenopus laevis extracts.
1999,
Pubmed
,
Xenbase Goldberg,
Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly.
1997,
Pubmed
,
Xenbase Goldberg,
Xenopus lamin B3 has a direct role in the assembly of a replication competent nucleus: evidence from cell-free egg extracts.
1995,
Pubmed
,
Xenbase Goldman,
Accumulation of mutant lamin A causes progressive changes in nuclear architecture in Hutchinson-Gilford progeria syndrome.
2004,
Pubmed Haraguchi,
Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes.
2000,
Pubmed Hawryluk-Gara,
Nup53 is required for nuclear envelope and nuclear pore complex assembly.
2008,
Pubmed
,
Xenbase Hetzer,
Pushing the envelope: structure, function, and dynamics of the nuclear periphery.
2005,
Pubmed Hetzer,
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
2001,
Pubmed
,
Xenbase Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase Howe,
A developmental timer regulates degradation of cyclin E1 at the midblastula transition during Xenopus embryogenesis.
1996,
Pubmed
,
Xenbase Hu,
Membrane proteins of the endoplasmic reticulum induce high-curvature tubules.
2008,
Pubmed Jun,
Sec18p and Vam7p remodel trans-SNARE complexes to permit a lipid-anchored R-SNARE to support yeast vacuole fusion.
2007,
Pubmed Larijani,
Role for phosphatidylinositol in nuclear envelope formation.
2001,
Pubmed Liu,
Functional association of Sun1 with nuclear pore complexes.
2007,
Pubmed Liu,
Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.
2000,
Pubmed Lohka,
The reconstitution of nuclear envelopes in cell-free extracts.
1988,
Pubmed Lohka,
Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
1984,
Pubmed
,
Xenbase Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase Ma,
Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta.
2007,
Pubmed
,
Xenbase Macaulay,
Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA.
1996,
Pubmed
,
Xenbase Maeshima,
Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins.
2006,
Pubmed Mansfeld,
The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells.
2006,
Pubmed
,
Xenbase Martens,
How synaptotagmin promotes membrane fusion.
2007,
Pubmed Mattaj,
Sorting out the nuclear envelope from the endoplasmic reticulum.
2004,
Pubmed Miller,
Nuclear pore complex: structure, function, and regulation.
1991,
Pubmed Murakami,
Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos.
1998,
Pubmed
,
Xenbase Nakatogawa,
Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion.
2007,
Pubmed Newmeyer,
In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.
1986,
Pubmed
,
Xenbase Newport,
Characterization of the membrane binding and fusion events during nuclear envelope assembly using purified components.
1992,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Otter-Nilsson,
Cytosolic ATPases, p97 and NSF, are sufficient to mediate rapid membrane fusion.
1999,
Pubmed Powers,
Preparation and use of interphase Xenopus egg extracts.
2001,
Pubmed
,
Xenbase Prescott,
Palmitoylation of the synaptic vesicle fusion machinery.
2009,
Pubmed Pyrpasopoulou,
The lamin B receptor (LBR) provides essential chromatin docking sites at the nuclear envelope.
1996,
Pubmed Ramos,
Transmembrane proteins are not required for early stages of nuclear envelope assembly.
2006,
Pubmed Rizzo,
Cholesterol, triacylglicerols and phospholipids during Xenopus embryo development.
1994,
Pubmed
,
Xenbase Rothman,
The protein machinery of vesicle budding and fusion.
1996,
Pubmed Salpingidou,
NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes.
2008,
Pubmed
,
Xenbase Sapir,
Viral and developmental cell fusion mechanisms: conservation and divergence.
2008,
Pubmed Sasagawa,
In vitro nuclear assembly with affinity-purified nuclear envelope precursor vesicle fractions, PV1 and PV2.
1999,
Pubmed
,
Xenbase Scarcelli,
The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes.
2007,
Pubmed Segura-Totten,
Barrier-to-autointegration factor: major roles in chromatin decondensation and nuclear assembly.
2002,
Pubmed
,
Xenbase Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase Shen,
Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion.
2009,
Pubmed Smirnova,
Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells.
2001,
Pubmed Stavru,
NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes.
2006,
Pubmed Vigers,
A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs.
1991,
Pubmed
,
Xenbase Voeltz,
A class of membrane proteins shaping the tubular endoplasmic reticulum.
2006,
Pubmed Vogel,
Targeting of SNAP-25 to membranes is mediated by its association with the target SNARE syntaxin.
2000,
Pubmed Whyte,
Vesicle tethering complexes in membrane traffic.
2002,
Pubmed Wiese,
Nuclear envelope assembly in Xenopus extracts visualized by scanning EM reveals a transport-dependent 'envelope smoothing' event.
1997,
Pubmed
,
Xenbase Wilson,
A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro.
1988,
Pubmed
,
Xenbase Xie,
Expression and subcellular localization of syntaxin 11 in human neutrophils.
2009,
Pubmed Zhang,
Structural basis for recruitment of mitochondrial fission complexes by Fis1.
2007,
Pubmed Zhang,
Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
