Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Voltage-gated Na(+) channel (VGSC) beta1 and beta2 subunits are multifunctional, serving as both channel modulators and cell adhesion molecules (CAMs). The purpose of this study was to determine whether VGSC beta3 subunits function as CAMs. The beta3 extracellular domain is highly homologous to beta1, suggesting that beta3 may also be a functional CAM. We investigated the trans homophilic cell adhesive properties of beta3, its association with the beta1-interacting CAM contactin, as well as its ability to interact with the cytoskeletal protein ankyrin. Our results demonstrate that, unlike beta1, beta3 does not participate in trans homophilic cell-cell adhesion or associate with contactin. Further, beta3 does not associate with ankyrin(G) in a heterologous system. Previous studies have shown that beta3 interacts with the CAM neurofascin-186 but not with VGSC beta1. Taken together, these findings suggest that, although beta1 and beta3 exhibit similar channel modulatory properties in heterologous systems, these subunits differ with regard to their homophilic and heterophilic CAM binding profiles.
???displayArticle.pubmedLink???
19596049 ???displayArticle.pmcLink???PMC2744945 ???displayArticle.link???Neurosci Lett ???displayArticle.grants???[+]
Adachi,
Identification of SCN3B as a novel p53-inducible proapoptotic gene.
2004, Pubmed
Adachi,
Identification of SCN3B as a novel p53-inducible proapoptotic gene.
2004,
Pubmed Adelman,
Proteins that interact with the pore-forming subunits of voltage-gated ion channels.
1995,
Pubmed Bouzidi,
Interaction of the Nav1.2a subunit of the voltage-dependent sodium channel with nodal ankyrinG. In vitro mapping of the interacting domains and association in synaptosomes.
2002,
Pubmed Brackenbury,
An emerging role for voltage-gated Na+ channels in cellular migration: regulation of central nervous system development and potentiation of invasive cancers.
2008,
Pubmed Brackenbury,
Voltage-gated Na+ channels: potential for beta subunits as therapeutic targets.
2008,
Pubmed Brackenbury,
Voltage-gated Na+ channel beta1 subunit-mediated neurite outgrowth requires Fyn kinase and contributes to postnatal CNS development in vivo.
2008,
Pubmed Catterall,
Cellular and molecular biology of voltage-gated sodium channels.
1992,
Pubmed Chen,
Mice lacking sodium channel beta1 subunits display defects in neuronal excitability, sodium channel expression, and nodal architecture.
2004,
Pubmed Chioni,
A novel adhesion molecule in human breast cancer cells: voltage-gated Na+ channel beta1 subunit.
2009,
Pubmed Davis,
Sodium channel beta1 subunits promote neurite outgrowth in cerebellar granule neurons.
2004,
Pubmed Davis,
Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FNIII domain-) and NrCAM at nodal axon segments.
1996,
Pubmed Davis,
Ankyrin binding activity shared by the neurofascin/L1/NrCAM family of nervous system cell adhesion molecules.
1994,
Pubmed Fein,
Cloning and expression of a zebrafish SCN1B ortholog and identification of a species-specific splice variant.
2007,
Pubmed
,
Xenbase Fein,
scn1bb, a zebrafish ortholog of SCN1B expressed in excitable and nonexcitable cells, affects motor neuron axon morphology and touch sensitivity.
2008,
Pubmed Hakim,
Scn3b knockout mice exhibit abnormal ventricular electrophysiological properties.
2008,
Pubmed Hedstrom,
Intrinsic and extrinsic determinants of ion channel localization in neurons.
2006,
Pubmed Isom,
The role of sodium channels in cell adhesion.
2002,
Pubmed Isom,
Na+ channel subunits and Ig domains.
1996,
Pubmed Isom,
Auxiliary subunits of voltage-gated ion channels.
1994,
Pubmed Kaczmarek,
Non-conducting functions of voltage-gated ion channels.
2006,
Pubmed Kazarinova-Noyes,
Contactin associates with Na+ channels and increases their functional expression.
2001,
Pubmed Kim,
BACE1 regulates voltage-gated sodium channels and neuronal activity.
2007,
Pubmed Lemaillet,
Identification of a conserved ankyrin-binding motif in the family of sodium channel alpha subunits.
2003,
Pubmed Levitan,
Signaling protein complexes associated with neuronal ion channels.
2006,
Pubmed Lopez-Santiago,
Sodium channel Scn1b null mice exhibit prolonged QT and RR intervals.
2007,
Pubmed Malhotra,
Sodium channel beta subunits mediate homophilic cell adhesion and recruit ankyrin to points of cell-cell contact.
2000,
Pubmed Malhotra,
Structural requirements for interaction of sodium channel beta 1 subunits with ankyrin.
2002,
Pubmed McCormick,
Molecular determinants of Na+ channel function in the extracellular domain of the beta1 subunit.
1998,
Pubmed
,
Xenbase McEwen,
Heterophilic interactions of sodium channel beta1 subunits with axonal and glial cell adhesion molecules.
2004,
Pubmed McEwen,
Sodium channel beta1 subunit-mediated modulation of Nav1.2 currents and cell surface density is dependent on interactions with contactin and ankyrin.
2004,
Pubmed Meadows,
Sodium channels as macromolecular complexes: implications for inherited arrhythmia syndromes.
2005,
Pubmed Morgan,
beta 3: an additional auxiliary subunit of the voltage-sensitive sodium channel that modulates channel gating with distinct kinetics.
2000,
Pubmed
,
Xenbase O'Malley,
Loss of Na+ channel beta2 subunits is neuroprotective in a mouse model of multiple sclerosis.
2009,
Pubmed Ratcliffe,
Sodium channel beta1 and beta3 subunits associate with neurofascin through their extracellular immunoglobulin-like domain.
2001,
Pubmed Shah,
Developmental expression of the novel voltage-gated sodium channel auxiliary subunit beta3, in rat CNS.
2001,
Pubmed
,
Xenbase Shapiro,
Crystal structure of the extracellular domain from P0, the major structural protein of peripheral nerve myelin.
1996,
Pubmed Srinivasan,
Interaction of voltage-gated sodium channels with the extracellular matrix molecules tenascin-C and tenascin-R.
1998,
Pubmed Vaughn,
The (Greek) key to structures of neural adhesion molecules.
1996,
Pubmed Warner,
Clinical phenotypes of different MPZ (P0) mutations may include Charcot-Marie-Tooth type 1B, Dejerine-Sottas, and congenital hypomyelination.
1996,
Pubmed Williams,
The immunoglobulin superfamily--domains for cell surface recognition.
1988,
Pubmed Xiao,
Tenascin-R is a functional modulator of sodium channel beta subunits.
1999,
Pubmed
,
Xenbase
