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Neuron
2009 Nov 12;643:355-366. doi: 10.1016/j.neuron.2009.09.018.
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Extracellular Engrailed participates in the topographic guidance of retinal axons in vivo.
Wizenmann A, Brunet I, Lam J, Sonnier L, Beurdeley M, Zarbalis K, Weisenhorn-Vogt D, Weinl C, Dwivedy A, Joliot A, Wurst W, Holt C, Prochiantz A.
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Engrailed transcription factors regulate the expression of guidance cues that pattern retinal axon terminals in the dorsal midbrain. They also act directly to guide axon growth in vitro. We show here that an extracellular En gradient exists in the tectum along the anterior-posterior axis. Neutralizing extracellular Engrailed in vivo with antibodies expressed in the tectum causes temporal axons to map aberrantly to the posterior tectum in chick and Xenopus. Furthermore, posterior membranes from wild-type tecta incubated with anti-Engrailed antibodies or posterior membranes from Engrailed-1 knockout mice exhibit diminished repulsive activity for temporal axons. Since EphrinAs play a major role in anterior-posterior mapping, we tested whether Engrailed cooperates with EphrinA5 in vitro. We find that Engrailed restores full repulsion to axons given subthreshold doses of EphrinA5. Collectively, our results indicate that extracellular Engrailed contributes to retinotectal mapping in vivo by modulating the sensitivity of growth cones to EphrinA.
Auerbach,
A simple procedure for the long-term cultivation of chicken embryos.
1974, Pubmed
Auerbach,
A simple procedure for the long-term cultivation of chicken embryos.
1974,
Pubmed Auf der Maur,
Antigen-independent selection of intracellular stable antibody frameworks.
2004,
Pubmed Bovolenta,
Morphogen signaling at the vertebrate growth cone: a few cases or a general strategy?
2005,
Pubmed Brunet,
The topological role of homeoproteins in the developing central nervous system.
2007,
Pubmed Brunet,
The transcription factor Engrailed-2 guides retinal axons.
2005,
Pubmed
,
Xenbase Buhusi,
L1 interaction with ankyrin regulates mediolateral topography in the retinocollicular projection.
2008,
Pubmed Cheng,
Complementary gradients in expression and binding of ELF-1 and Mek4 in development of the topographic retinotectal projection map.
1995,
Pubmed Chien,
Navigational errors made by growth cones without filopodia in the embryonic Xenopus brain.
1993,
Pubmed
,
Xenbase Ciossek,
Eph receptor-ligand interactions are necessary for guidance of retinal ganglion cell axons in vitro.
1998,
Pubmed Claudepierre,
Implication of neuropilin 2/semaphorin 3F in retinocollicular map formation.
2008,
Pubmed Di Nardo,
Dendritic localization and activity-dependent translation of Engrailed1 transcription factor.
2007,
Pubmed Drescher,
In vitro guidance of retinal ganglion cell axons by RAGS, a 25 kDa tectal protein related to ligands for Eph receptor tyrosine kinases.
1995,
Pubmed Dütting,
Transplantations of the chick eye anlage reveal an early determination of nasotemporal polarity.
1995,
Pubmed Falk,
Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus.
2007,
Pubmed
,
Xenbase Feldheim,
Loss-of-function analysis of EphA receptors in retinotectal mapping.
2004,
Pubmed Feldheim,
Genetic analysis of ephrin-A2 and ephrin-A5 shows their requirement in multiple aspects of retinocollicular mapping.
2000,
Pubmed Flanagan,
Neural map specification by gradients.
2006,
Pubmed Friedman,
Retroviral misexpression of engrailed genes in the chick optic tectum perturbs the topographic targeting of retinal axons.
1996,
Pubmed Frisén,
Ephrin-A5 (AL-1/RAGS) is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system.
1998,
Pubmed Furrer,
Slit and Robo control the development of dendrites in Drosophila CNS.
2007,
Pubmed Gingras,
eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation.
1999,
Pubmed Haas,
Single-cell electroporation for gene transfer in vivo.
2001,
Pubmed
,
Xenbase Hanks,
Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2.
1995,
Pubmed Hansen,
Retinal axon response to ephrin-as shows a graded, concentration-dependent transition from growth promotion to inhibition.
2004,
Pubmed Hemmati-Brivanlou,
Cephalic expression and molecular characterization of Xenopus En-2.
1991,
Pubmed
,
Xenbase Holcman,
Modeling homeoprotein intercellular transfer unveils a parsimonious mechanism for gradient and boundary formation in early brain development.
2007,
Pubmed Hornberger,
Modulation of EphA receptor function by coexpressed ephrinA ligands on retinal ganglion cell axons.
1999,
Pubmed Itasaki,
A role for gradient en expression in positional specification on the optic tectum.
1996,
Pubmed Joliot,
Association of Engrailed homeoproteins with vesicles presenting caveolae-like properties.
1997,
Pubmed Joliot,
Transduction peptides: from technology to physiology.
2004,
Pubmed Knöll,
Stripe assay to examine axonal guidance and cell migration.
2007,
Pubmed Lesaffre,
Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish.
2007,
Pubmed Logan,
Rostral optic tectum acquires caudal characteristics following ectopic engrailed expression.
1996,
Pubmed Lohof,
Asymmetric modulation of cytosolic cAMP activity induces growth cone turning.
1992,
Pubmed
,
Xenbase Mann,
Topographic mapping in dorsoventral axis of the Xenopus retinotectal system depends on signaling through ephrin-B ligands.
2002,
Pubmed
,
Xenbase McFarlane,
Inhibition of FGF receptor activity in retinal ganglion cell axons causes errors in target recognition.
1996,
Pubmed
,
Xenbase McLaughlin,
Molecular gradients and development of retinotopic maps.
2005,
Pubmed Mueller,
Partial respecification of nasotemporal polarity in double-temporal chick and chimeric chick-quail eyes.
1998,
Pubmed Nakamura,
The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.
1997,
Pubmed O'Leary,
Patterning centers, regulatory genes and extrinsic mechanisms controlling arealization of the neocortex.
2002,
Pubmed O'Leary,
Mechanisms of retinotopic map development: Ephs, ephrins, and spontaneous correlated retinal activity.
2005,
Pubmed Pfeiffenberger,
Ephrin-As and patterned retinal activity act together in the development of topographic maps in the primary visual system.
2006,
Pubmed Raper,
The enrichment of a neuronal growth cone collapsing activity from embryonic chick brain.
1990,
Pubmed Sakaguchi,
Growth cone interactions with a glial cell line from embryonic Xenopus retina.
1989,
Pubmed
,
Xenbase Schmid,
L1 and NCAM adhesion molecules as signaling coreceptors in neuronal migration and process outgrowth.
2008,
Pubmed Shigetani,
En-2 regulates the expression of the ligands for Eph type tyrosine kinases in chick embryonic tectum.
1997,
Pubmed Sonnier,
Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1.
2007,
Pubmed SPERRY,
CHEMOAFFINITY IN THE ORDERLY GROWTH OF NERVE FIBER PATTERNS AND CONNECTIONS.
1963,
Pubmed Strochlic,
A role for S1P signalling in axon guidance in the Xenopus visual system.
2008,
Pubmed
,
Xenbase Strube,
Enhanced intracellular stability of sFv-Fc fusion intrabodies.
2004,
Pubmed Sugiyama,
Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity.
2008,
Pubmed Swartz,
Sparking new frontiers: using in vivo electroporation for genetic manipulations.
2001,
Pubmed
,
Xenbase Thanos,
Specific transcellular carbocyanine-labelling of rat retinal microglia during injury-induced neuronal degeneration.
1991,
Pubmed Vielmetter,
In vitro assay to test differential substrate affinities of growing axons and migratory cells.
1990,
Pubmed Walter,
Recognition of position-specific properties of tectal cell membranes by retinal axons in vitro.
1987,
Pubmed Walter,
Avoidance of posterior tectal membranes by temporal retinal axons.
1987,
Pubmed Weinl,
Responses of temporal retinal growth cones to ephrinA5-coated beads.
2005,
Pubmed Wilkinson,
Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts.
1993,
Pubmed Wurst,
Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum.
1994,
Pubmed Yates,
Topographic-specific axon branching controlled by ephrin-As is the critical event in retinotectal map development.
2001,
Pubmed
