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EMBO J
2010 Jan 20;292:387-97. doi: 10.1038/emboj.2009.337.
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Enforcing temporal control of maternal mRNA translation during oocyte cell-cycle progression.
Arumugam K, Wang Y, Hardy LL, MacNicol MC, MacNicol AM.
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Meiotic cell-cycle progression in progesterone-stimulated Xenopus oocytes requires that the translation of pre-existing maternal mRNAs occur in a strict temporal order. Timing of translation is regulated through elements within the mRNA 3' untranslated region (3' UTR), which respond to cell cycle-dependant signalling. One element that has been previously implicated in the temporal control of mRNA translation is the cytoplasmic polyadenylation element (CPE). In this study, we show that the CPE does not direct early mRNA translation. Rather, early translation is directed through specific early factors, including the Musashi-binding element (MBE) and the MBE-binding protein, Musashi. Our findings indicate that although the cyclin B5 3' UTR contains both CPEs and an MBE, the MBE is the critical regulator of early translation. The cyclin B2 3' UTR contains CPEs, but lacks an MBE and is translationally activated late in maturation. Finally, utilizing antisense oligonucleotides to attenuate endogenous Musashi synthesis, we show that Musashi is critical for the initiation of early class mRNA translation and for the subsequent activation of CPE-dependant mRNA translation.
Andrésson,
The kinase Eg2 is a component of the Xenopus oocyte progesterone-activated signaling pathway.
1998, Pubmed,
Xenbase
Andrésson,
The kinase Eg2 is a component of the Xenopus oocyte progesterone-activated signaling pathway.
1998,
Pubmed
,
Xenbase Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997,
Pubmed
,
Xenbase Barkoff,
Translational control of cyclin B1 mRNA during meiotic maturation: coordinated repression and cytoplasmic polyadenylation.
2000,
Pubmed
,
Xenbase Castro,
Involvement of Aurora A kinase during meiosis I-II transition in Xenopus oocytes.
2003,
Pubmed
,
Xenbase Charlesworth,
Musashi regulates the temporal order of mRNA translation during Xenopus oocyte maturation.
2006,
Pubmed
,
Xenbase Charlesworth,
A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation.
2002,
Pubmed
,
Xenbase Charlesworth,
The temporal control of Wee1 mRNA translation during Xenopus oocyte maturation is regulated by cytoplasmic polyadenylation elements within the 3'-untranslated region.
2000,
Pubmed
,
Xenbase Charlesworth,
Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus oocytes.
2004,
Pubmed
,
Xenbase Chen,
The beta subunit of CKII negatively regulates Xenopus oocyte maturation.
1997,
Pubmed
,
Xenbase Chen,
The casein kinase II beta subunit binds to Mos and inhibits Mos activity.
1997,
Pubmed
,
Xenbase de Moor,
The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes.
1997,
Pubmed
,
Xenbase Dupré,
Mos is not required for the initiation of meiotic maturation in Xenopus oocytes.
2002,
Pubmed
,
Xenbase Dworkin,
Mobilization of specific maternal RNA species into polysomes after fertilization in Xenopus laevis.
1985,
Pubmed
,
Xenbase Ferby,
A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes.
1999,
Pubmed
,
Xenbase Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase Frank-Vaillant,
Progesterone regulates the accumulation and the activation of Eg2 kinase in Xenopus oocytes.
2000,
Pubmed
,
Xenbase Freeman,
Meiotic induction by Xenopus cyclin B is accelerated by coexpression with mosXe.
1991,
Pubmed
,
Xenbase Furuno,
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
1994,
Pubmed
,
Xenbase Good,
Three new members of the RNP protein family in Xenopus.
1993,
Pubmed
,
Xenbase Gotoh,
Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade.
1995,
Pubmed
,
Xenbase Gross,
The critical role of the MAP kinase pathway in meiosis II in Xenopus oocytes is mediated by p90(Rsk).
2000,
Pubmed
,
Xenbase Gutierrez,
Meiotic regulation of the CDK activator RINGO/Speedy by ubiquitin-proteasome-mediated processing and degradation.
2006,
Pubmed
,
Xenbase Howard,
The mitogen-activated protein kinase signaling pathway stimulates mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation.
1999,
Pubmed
,
Xenbase Huang,
Dependence of Mos-induced Cdc2 activation on MAP kinase function in a cell-free system.
1996,
Pubmed
,
Xenbase Huang,
Ultrasensitivity in the mitogen-activated protein kinase cascade.
1996,
Pubmed
,
Xenbase Hyman,
Translational inactivation of ribosomal protein mRNAs during Xenopus oocyte maturation.
1988,
Pubmed
,
Xenbase Imai,
The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA.
2001,
Pubmed Keady,
MAPK interacts with XGef and is required for CPEB activation during meiosis in Xenopus oocytes.
2007,
Pubmed
,
Xenbase Kosako,
Requirement for the MAP kinase kinase/MAP kinase cascade in Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Kumagai,
Control of the Cdc2/cyclin B complex in Xenopus egg extracts arrested at a G2/M checkpoint with DNA synthesis inhibitors.
1995,
Pubmed
,
Xenbase Machaca,
Induction of maturation-promoting factor during Xenopus oocyte maturation uncouples Ca(2+) store depletion from store-operated Ca(2+) entry.
2002,
Pubmed
,
Xenbase Maton,
Cdc2-cyclin B triggers H3 kinase activation of Aurora-A in Xenopus oocytes.
2003,
Pubmed
,
Xenbase McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase Mendez,
Differential mRNA translation and meiotic progression require Cdc2-mediated CPEB destruction.
2002,
Pubmed
,
Xenbase Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase Murakami,
Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos.
1998,
Pubmed
,
Xenbase Nakajo,
Absence of Wee1 ensures the meiotic cell cycle in Xenopus oocytes.
2000,
Pubmed
,
Xenbase Padmanabhan,
Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.
2006,
Pubmed
,
Xenbase Palmer,
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
1998,
Pubmed
,
Xenbase Piqué,
A combinatorial code for CPE-mediated translational control.
2008,
Pubmed
,
Xenbase Prasad,
Mos 3' UTR regulatory differences underlie species-specific temporal patterns of Mos mRNA cytoplasmic polyadenylation and translational recruitment during oocyte maturation.
2008,
Pubmed
,
Xenbase Radford,
Translational control by cytoplasmic polyadenylation in Xenopus oocytes.
2008,
Pubmed
,
Xenbase Reverte,
CPEB degradation during Xenopus oocyte maturation requires a PEST domain and the 26S proteasome.
2001,
Pubmed
,
Xenbase Richter,
Breaking the code of polyadenylation-induced translation.
2008,
Pubmed
,
Xenbase Rosenthal,
Sequence-specific adenylations and deadenylations accompany changes in the translation of maternal messenger RNA after fertilization of Spisula oocytes.
1983,
Pubmed Roy,
Activation of p34cdc2 kinase by cyclin A.
1991,
Pubmed
,
Xenbase Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Sakakibara,
RNA-binding protein Musashi family: roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation.
2002,
Pubmed Sakakibara,
Rna-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS.
2001,
Pubmed
,
Xenbase Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase Tung,
Translational unmasking of Emi2 directs cytostatic factor arrest in meiosis II.
2007,
Pubmed
,
Xenbase Vassalli,
Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
1989,
Pubmed Wang,
A novel mRNA 3' untranslated region translational control sequence regulates Xenopus Wee1 mRNA translation.
2008,
Pubmed
,
Xenbase
