Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2010 Feb 03;293:532-45. doi: 10.1038/emboj.2009.379.
Show Gene links
Show Anatomy links
Identification of a novel Bves function: regulation of vesicular transport.
Hager HA, Roberts RJ, Cross EE, Proux-Gillardeaux V, Bader DM.
???displayArticle.abstract??? Blood vessel/epicardial substance (Bves) is a transmembrane protein that influences cell adhesion and motility through unknown mechanisms. We have discovered that Bves directly interacts with VAMP3, a SNARE protein that facilitates vesicular transport and specifically recycles transferrin and beta-1-integrin. Two independent assays document that cells expressing a mutated form of Bves are severely impaired in the recycling of these molecules, a phenotype consistent with disruption of VAMP3 function. Using Morpholino knockdown in Xenopus laevis, we demonstrate that elimination of Bves function specifically inhibits transferrin receptor recycling, and results in gastrulation defects previously reported with impaired integrin-dependent cell movements. Kymographic analysis of Bves-depleted primary and cultured cells reveals severe impairment of cell spreading and adhesion on fibronectin, indicative of disruption of integrin-mediated adhesion. Taken together, these data demonstrate that Bves interacts with VAMP3 and facilitates receptor recycling both in vitro and during early development. Thus, this study establishes a newly identified role for Bves in vesicular transport and reveals a novel, broadly applied mechanism governing SNARE protein function.
Andrée,
Isolation and characterization of the novel popeye gene family expressed in skeletal muscle and heart.
2000, Pubmed
Andrée,
Isolation and characterization of the novel popeye gene family expressed in skeletal muscle and heart.
2000,
Pubmed Andrée,
Mouse Pop1 is required for muscle regeneration in adult skeletal muscle.
2002,
Pubmed Bajno,
Focal exocytosis of VAMP3-containing vesicles at sites of phagosome formation.
2000,
Pubmed Bhattacharya,
Members of the synaptobrevin/vesicle-associated membrane protein (VAMP) family in Drosophila are functionally interchangeable in vivo for neurotransmitter release and cell viability.
2002,
Pubmed Borisovska,
v-SNAREs control exocytosis of vesicles from priming to fusion.
2005,
Pubmed Brand,
The Popeye domain-containing gene family.
2005,
Pubmed Breton,
Tetanus toxin-mediated cleavage of cellubrevin inhibits proton secretion in the male reproductive tract.
2000,
Pubmed Brunger,
Structure and function of SNARE and SNARE-interacting proteins.
2005,
Pubmed Bryan,
GEFT, a Rho family guanine nucleotide exchange factor, regulates neurite outgrowth and dendritic spine formation.
2004,
Pubmed Bryan,
The Rho-family guanine nucleotide exchange factor GEFT enhances retinoic acid- and cAMP-induced neurite outgrowth.
2006,
Pubmed Bryant,
The ins and outs of E-cadherin trafficking.
2004,
Pubmed Cai,
Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle.
2007,
Pubmed Caswell,
Integrin trafficking and the control of cell migration.
2006,
Pubmed Caswell,
Endocytic transport of integrins during cell migration and invasion.
2008,
Pubmed Davidson,
Integrin alpha5beta1 and fibronectin regulate polarized cell protrusions required for Xenopus convergence and extension.
2006,
Pubmed
,
Xenbase Deák,
Structural determinants of synaptobrevin 2 function in synaptic vesicle fusion.
2006,
Pubmed DeMali,
Integrin signaling to the actin cytoskeleton.
2003,
Pubmed Desclozeaux,
Active Rab11 and functional recycling endosome are required for E-cadherin trafficking and lumen formation during epithelial morphogenesis.
2008,
Pubmed DeSimone,
The Xenopus embryo as a model system for studies of cell migration.
2005,
Pubmed
,
Xenbase Dünnwald,
Detection of transient in vivo interactions between substrate and transporter during protein translocation into the endoplasmic reticulum.
1999,
Pubmed Etienne-Manneville,
Rho GTPases in cell biology.
2002,
Pubmed Fackler,
Cell motility through plasma membrane blebbing.
2008,
Pubmed Feng,
DNA methylation in tumor and matched normal tissues from non-small cell lung cancer patients.
2008,
Pubmed Fields,
v-SNARE cellubrevin is required for basolateral sorting of AP-1B-dependent cargo in polarized epithelial cells.
2007,
Pubmed Galli,
Tetanus toxin-mediated cleavage of cellubrevin impairs exocytosis of transferrin receptor-containing vesicles in CHO cells.
1994,
Pubmed Grosshans,
Rabs and their effectors: achieving specificity in membrane traffic.
2006,
Pubmed Guo,
A Rac/Cdc42-specific exchange factor, GEFT, induces cell proliferation, transformation, and migration.
2003,
Pubmed Hager,
Bves: ten years after.
2009,
Pubmed Hirohashi,
Inactivation of the E-cadherin-mediated cell adhesion system in human cancers.
1998,
Pubmed Holly,
Multiple roles of integrins in cell motility.
2000,
Pubmed Jahn,
SNAREs--engines for membrane fusion.
2006,
Pubmed Johnson,
Circus movements and blebbing locomotion in dissociated embryonic cells of an amphibian, Xenopus laevis.
1976,
Pubmed
,
Xenbase Johnson,
Mesodermal cell adhesion to fibronectin-rich fibrillar extracellular matrix is required for normal Rana pipiens gastrulation.
1993,
Pubmed Kawaguchi,
Identification of a novel intracellular interaction domain essential for Bves function.
2008,
Pubmed Keller,
The cellular basis of epiboly: an SEM study of deep-cell rearrangement during gastrulation in Xenopus laevis.
1980,
Pubmed
,
Xenbase Knight,
Membrane topology of Bves/Pop1A, a cell adhesion molecule that displays dynamic changes in cellular distribution during development.
2003,
Pubmed Kumano,
Revisions to the Xenopus gastrula fate map: implications for mesoderm induction and patterning.
2002,
Pubmed
,
Xenbase Lanzetti,
Actin in membrane trafficking.
2007,
Pubmed Leabu,
Membrane fusion in cells: molecular machinery and mechanisms.
2006,
Pubmed Lin,
Blood vessel/epicardial substance (bves) expression, essential for embryonic development, is down regulated by Grk/EFGR signalling.
2007,
Pubmed Luftman,
Silencing of VAMP3 inhibits cell migration and integrin-mediated adhesion.
2009,
Pubmed Marsden,
Integrin-ECM interactions regulate cadherin-dependent cell adhesion and are required for convergent extension in Xenopus.
2003,
Pubmed
,
Xenbase Marsden,
Regulation of cell polarity, radial intercalation and epiboly in Xenopus: novel roles for integrin and fibronectin.
2001,
Pubmed
,
Xenbase McCarthy,
Allen Brain Atlas maps 21,000 genes of the mouse brain.
2006,
Pubmed McMahon,
Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein.
1993,
Pubmed Mellman,
Coordinated protein sorting, targeting and distribution in polarized cells.
2008,
Pubmed Na,
Differential regulation of cell adhesive functions by integrin alpha subunit cytoplasmic tails in vivo.
2003,
Pubmed
,
Xenbase Osler,
Bves expression during avian embryogenesis.
2004,
Pubmed Osler,
Bves modulates epithelial integrity through an interaction at the tight junction.
2005,
Pubmed Osler,
Bves, a member of the Popeye domain-containing gene family.
2006,
Pubmed
,
Xenbase Pfeffer,
Unsolved mysteries in membrane traffic.
2007,
Pubmed Polgár,
Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion.
2002,
Pubmed Proux-Gillardeaux,
Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion.
2005,
Pubmed Ramos,
Xenopus embryonic cell adhesion to fibronectin: position-specific activation of RGD/synergy site-dependent migratory behavior at gastrulation.
1996,
Pubmed
,
Xenbase Ramos,
Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation.
1996,
Pubmed
,
Xenbase Ren,
Migrating anterior mesoderm cells and intercalating trunk mesoderm cells have distinct responses to Rho and Rac during Xenopus gastrulation.
2006,
Pubmed
,
Xenbase Ridley,
Rho proteins: linking signaling with membrane trafficking.
2001,
Pubmed Ripley,
Bves is expressed in the epithelial components of the retina, lens, and cornea.
2004,
Pubmed Ripley,
Xbves is a regulator of epithelial movement during early Xenopus laevis development.
2006,
Pubmed
,
Xenbase Schwartz,
Signaling networks linking integrins and rho family GTPases.
2000,
Pubmed Segev,
Ypt/rab gtpases: regulators of protein trafficking.
2001,
Pubmed Selchow,
Structure and cytoskeletal organization of migratory mesoderm cells from the Xenopus gastrula.
1997,
Pubmed
,
Xenbase Shook,
Mechanisms, mechanics and function of epithelial-mesenchymal transitions in early development.
2003,
Pubmed
,
Xenbase Skalski,
SNARE-mediated trafficking of alpha5beta1 integrin is required for spreading in CHO cells.
2005,
Pubmed Smith,
Bves directly interacts with GEFT, and controls cell shape and movement through regulation of Rac1/Cdc42 activity.
2008,
Pubmed Smith,
Mesoderm induction and the control of gastrulation in Xenopus laevis: the roles of fibronectin and integrins.
1990,
Pubmed
,
Xenbase Smith,
Characterization of Bves expression during mouse development using newly generated immunoreagents.
2006,
Pubmed Symons,
Control of vesicular trafficking by Rho GTPases.
2003,
Pubmed Tajika,
VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration.
2007,
Pubmed Tayeb,
Inhibition of SNARE-mediated membrane traffic impairs cell migration.
2005,
Pubmed Torlopp,
Comparative analysis of mRNA and protein expression of Popdc1 (Bves) during early development in the chick embryo.
2006,
Pubmed Vasavada,
Developmental expression of Pop1/Bves.
2004,
Pubmed Wada,
Bves: prototype of a new class of cell adhesion molecules expressed during coronary artery development.
2001,
Pubmed Wallingford,
Dishevelled controls cell polarity during Xenopus gastrulation.
2000,
Pubmed
,
Xenbase Winklbauer,
Directional mesoderm cell migration in the Xenopus gastrula.
1991,
Pubmed
,
Xenbase Yap,
Making and breaking contacts: the cellular biology of cadherin regulation.
2007,
Pubmed
