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???displayArticle.abstract??? Ngn3 is a bHLH transcription factor critical for the specification of endocrine cells in the pancreatic Islets of Langerhans. Previous studies in mouse embryos have reported transient expression of Ngn3 in scattered cells within the developing pancreatic epithelium during midgestation (Schwitzgebel et al. [2000] Development 127:3533-3542). Specifically, these Ngn3-expressing cells have been shown to be progenitor cells fated to give rise to islet endocrine cells (Gradwohl et al. [2000] Proc Natl Acad Sci USA 97:1607-1611). Here, we characterize the expression of Ngn3 transcripts and protein throughout pancreatic development. Interestingly, we identify and define a dramatic and previously unnoticed gap in developmental Ngn3 expression. We show that both Ngn3 transcript and protein expression occur in two distinct temporal waves, the first occurring early from approximately E8.5 to E11.0, and the second initiating at approximately E12.0. Strikingly, this observed biphasic expression correlates with the "first" and "second" transitions, which encompass two distinct waves of embryonic endocrine differentiation. In addition, our studies demonstrate that Ngn3 transcripts are markedly more widespread in the pancreatic epithelium than NGN3 protein, indicating that post-transcriptional regulation is likely to play a critical role during endocrine differentiation.
Ahlgren,
The morphogenesis of the pancreatic mesenchyme is uncoupled from that of the pancreatic epithelium in IPF1/PDX1-deficient mice.
1996, Pubmed
Ahlgren,
The morphogenesis of the pancreatic mesenchyme is uncoupled from that of the pancreatic epithelium in IPF1/PDX1-deficient mice.
1996,
Pubmed Apelqvist,
Notch signalling controls pancreatic cell differentiation.
1999,
Pubmed
,
Xenbase Artner,
MafB is required for islet beta cell maturation.
2007,
Pubmed Beaupain,
[An immunocytological study of differentiation of the endocrine pancreas of the chick embryo. II. Glucagon].
1974,
Pubmed Burlison,
Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells.
2008,
Pubmed D'Amour,
Efficient differentiation of human embryonic stem cells to definitive endoderm.
2005,
Pubmed D'Amour,
Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells.
2006,
Pubmed Duvillié,
The mesenchyme controls the timing of pancreatic beta-cell differentiation.
2006,
Pubmed Fujitani,
Targeted deletion of a cis-regulatory region reveals differential gene dosage requirements for Pdx1 in foregut organ differentiation and pancreas formation.
2006,
Pubmed Gittes,
Onset of cell-specific gene expression in the developing mouse pancreas.
1992,
Pubmed GOLOSOW,
Epitheliomesenchymal interaction in pancreatic morphogenesis.
1962,
Pubmed Gradwohl,
neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas.
2000,
Pubmed
,
Xenbase Grapin-Botton,
Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes.
2001,
Pubmed Gu,
Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors.
2002,
Pubmed Guz,
Expression of murine STF-1, a putative insulin gene transcription factor, in beta cells of pancreas, duodenal epithelium and pancreatic exocrine and endocrine progenitors during ontogeny.
1995,
Pubmed
,
Xenbase Herrera,
Two transgenic approaches to define the cell lineages in endocrine pancreas development.
1998,
Pubmed Herrera,
Adult insulin- and glucagon-producing cells differentiate from two independent cell lineages.
2000,
Pubmed Herrera,
Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene.
1991,
Pubmed Huang,
Regulation of the pancreatic islet-specific gene BETA2 (neuroD) by neurogenin 3.
2000,
Pubmed
,
Xenbase Jensen,
Gene regulatory factors in pancreatic development.
2004,
Pubmed Johansson,
Temporal control of neurogenin3 activity in pancreas progenitors reveals competence windows for the generation of different endocrine cell types.
2007,
Pubmed Jonsson,
Insulin-promoter-factor 1 is required for pancreas development in mice.
1994,
Pubmed Jørgensen,
An illustrated review of early pancreas development in the mouse.
2007,
Pubmed Kim,
Signaling and transcriptional control of pancreatic organogenesis.
2002,
Pubmed Kroon,
Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo.
2008,
Pubmed Li,
Selective agenesis of the dorsal pancreas in mice lacking homeobox gene Hlxb9.
1999,
Pubmed Li,
Persistent expression of Hlxb9 in the pancreatic epithelium impairs pancreatic development.
2001,
Pubmed Murtaugh,
Pancreas and beta-cell development: from the actual to the possible.
2007,
Pubmed Murtaugh,
Genes, signals, and lineages in pancreas development.
2003,
Pubmed Nishimura,
A switch from MafB to MafA expression accompanies differentiation to pancreatic beta-cells.
2006,
Pubmed Offield,
PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum.
1996,
Pubmed
,
Xenbase Pictet,
An ultrastructural analysis of the developing embryonic pancreas.
1972,
Pubmed Schonhoff,
Neurogenin 3-expressing progenitor cells in the gastrointestinal tract differentiate into both endocrine and non-endocrine cell types.
2004,
Pubmed Schwitzgebel,
Expression of neurogenin3 reveals an islet cell precursor population in the pancreas.
2000,
Pubmed Spooner,
The development of the dorsal and ventral mammalian pancreas in vivo and in vitro.
1970,
Pubmed Stefan,
Quantitation of endocrine cell content in the pancreas of nondiabetic and diabetic humans.
1982,
Pubmed Upchurch,
Expression of peptide YY in all four islet cell types in the developing mouse pancreas suggests a common peptide YY-producing progenitor.
1994,
Pubmed van Eyll,
Eph receptors and their ephrin ligands are expressed in developing mouse pancreas.
2006,
Pubmed Wang,
A novel zebrafish bHLH gene, neurogenin3, is expressed in the hypothalamus.
2001,
Pubmed Zhou,
A multipotent progenitor domain guides pancreatic organogenesis.
2007,
Pubmed
