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Mol Biol Cell
2009 Aug 01;2015:3491-502. doi: 10.1091/mbc.e09-05-0370.
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Molecular distinctions between Aurora A and B: a single residue change transforms Aurora A into correctly localized and functional Aurora B.
Hans F, Skoufias DA, Dimitrov S, Margolis RL.
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Aurora A and Aurora B, paralogue mitotic kinases, share highly similar primary sequence. Both are important to mitotic progression, but their localizations and functions are distinct. We have combined shRNA suppression with overexpression of Aurora mutants to address the cause of the distinction between Aurora A and Aurora B. Aurora A residue glycine 198 (G198), mutated to asparagine to mimic the aligned asparagine 142 (N142) of Aurora B, causes Aurora A to bind the Aurora B binding partner INCENP but not the Aurora A binding partner TPX2. The mutant Aurora A rescues Aurora B mitotic function. We conclude that binding to INCENP is alone critical to the distinct function of Aurora B. Although G198 of Aurora A is required for TPX2 binding, N142G Aurora B retains INCENP binding and Aurora B function. Thus, although a single residue change transforms Aurora A, the reciprocal mutation of Aurora B does not create Aurora A function. An Aurora A-Delta120 N-terminal truncation construct reinforces Aurora A similarity to Aurora B, because it does not associate with centrosomes but instead associates with kinetochores.
Adams,
INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow.
2000, Pubmed,
Xenbase
Adams,
INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow.
2000,
Pubmed
,
Xenbase Anand,
AURORA-A amplification overrides the mitotic spindle assembly checkpoint, inducing resistance to Taxol.
2003,
Pubmed Andrews,
Mitotic mechanics: the auroras come into view.
2003,
Pubmed
,
Xenbase Barr,
Aurora-A: the maker and breaker of spindle poles.
2007,
Pubmed Bayliss,
Determinants for Aurora-A activation and Aurora-B discrimination by TPX2.
2004,
Pubmed
,
Xenbase Bayliss,
Structural basis of Aurora-A activation by TPX2 at the mitotic spindle.
2003,
Pubmed
,
Xenbase Berman,
The Protein Data Bank.
2000,
Pubmed Bischoff,
The Aurora/Ipl1p kinase family: regulators of chromosome segregation and cytokinesis.
1999,
Pubmed Bischoff,
A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers.
1998,
Pubmed Bishop,
Phosphorylation of the carboxyl terminus of inner centromere protein (INCENP) by the Aurora B Kinase stimulates Aurora B kinase activity.
2002,
Pubmed Bolton,
Aurora B kinase exists in a complex with survivin and INCENP and its kinase activity is stimulated by survivin binding and phosphorylation.
2002,
Pubmed
,
Xenbase Carmena,
The cellular geography of aurora kinases.
2003,
Pubmed
,
Xenbase Ciferri,
Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex.
2008,
Pubmed Cooke,
The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosis.
1987,
Pubmed Dutertre,
On the role of aurora-A in centrosome function.
2002,
Pubmed Eyers,
Regulation of Xenopus Aurora A activation by TPX2.
2004,
Pubmed
,
Xenbase Eyers,
The Aurora A and Aurora B protein kinases: a single amino acid difference controls intrinsic activity and activation by TPX2.
2005,
Pubmed
,
Xenbase Fu,
Roles of Aurora kinases in mitosis and tumorigenesis.
2007,
Pubmed Fuller,
Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient.
2008,
Pubmed
,
Xenbase Giet,
Aurora kinases, aneuploidy and cancer, a coincidence or a real link?
2005,
Pubmed Giet,
Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases.
1999,
Pubmed Giet,
The non-catalytic domain of the Xenopus laevis auroraA kinase localises the protein to the centrosome.
2001,
Pubmed
,
Xenbase Goto,
Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensation.
2002,
Pubmed
,
Xenbase Hagemeier,
Functional interaction between the HCMV IE2 transactivator and the retinoblastoma protein.
1994,
Pubmed Hannak,
Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans.
2001,
Pubmed Hirota,
Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells.
2003,
Pubmed
,
Xenbase Honda,
Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis.
2003,
Pubmed Jelluma,
Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment.
2008,
Pubmed Jeyaprakash,
Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together.
2007,
Pubmed Kabsch,
Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features.
1983,
Pubmed Kaitna,
Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis.
2000,
Pubmed Kang,
The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition.
2002,
Pubmed
,
Xenbase Keniry,
Identification of p21-activated kinase specificity determinants in budding yeast: a single amino acid substitution imparts Ste20 specificity to Cla4.
2003,
Pubmed Klein,
Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP.
2006,
Pubmed Kufer,
Human TPX2 is required for targeting Aurora-A kinase to the spindle.
2002,
Pubmed
,
Xenbase Kunitoku,
CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function.
2003,
Pubmed Liu,
Aurora A, mitotic entry, and spindle bipolarity.
2006,
Pubmed
,
Xenbase Luger,
Expression and purification of recombinant histones and nucleosome reconstitution.
1999,
Pubmed Macůrek,
Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery.
2008,
Pubmed Meraldi,
Aurora kinases link chromosome segregation and cell division to cancer susceptibility.
2004,
Pubmed Meraldi,
Aurora-A overexpression reveals tetraploidization as a major route to centrosome amplification in p53-/- cells.
2002,
Pubmed Nowakowski,
Structures of the cancer-related Aurora-A, FAK, and EphA2 protein kinases from nanovolume crystallography.
2002,
Pubmed
,
Xenbase Ohashi,
Phospho-regulation of human protein kinase Aurora-A: analysis using anti-phospho-Thr288 monoclonal antibodies.
2006,
Pubmed Pan,
Cyclic AMP-dependent protein kinase regulates pseudohyphal differentiation in Saccharomyces cerevisiae.
1999,
Pubmed Rosasco-Nitcher,
Centromeric Aurora-B activation requires TD-60, microtubules, and substrate priming phosphorylation.
2008,
Pubmed
,
Xenbase Roussel,
CRYStallize: a crystallographic symmetry display and handling subpackage in TOM/FRODO.
1990,
Pubmed Ruchaud,
Chromosomal passengers: conducting cell division.
2007,
Pubmed Satinover,
Activation of Aurora-A kinase by protein phosphatase inhibitor-2, a bifunctional signaling protein.
2004,
Pubmed
,
Xenbase Scrittori,
A small C-terminal sequence of Aurora B is responsible for localization and function.
2005,
Pubmed Sessa,
Mechanism of Aurora B activation by INCENP and inhibition by hesperadin.
2005,
Pubmed
,
Xenbase Soncini,
PHA-680632, a novel Aurora kinase inhibitor with potent antitumoral activity.
2006,
Pubmed Tien,
Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model.
2004,
Pubmed Vader,
Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody.
2006,
Pubmed Zhou,
Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation.
1998,
Pubmed
