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J Virol
2010 May 01;8410:4912-22. doi: 10.1128/JVI.02486-09.
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Innate immune responses and permissiveness to ranavirus infection of peritoneal leukocytes in the frog Xenopus laevis.
Morales HD, Abramowitz L, Gertz J, Sowa J, Vogel A, Robert J.
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Ranaviruses such as frog virus 3 ([FV3] family Iridoviridae) are increasingly prevalent pathogens that infect reptiles, amphibians, and fish worldwide. Whereas studies in the frog Xenopus laevis have revealed the critical involvement of CD8 T-cell and antibody responses in host resistance to FV3, little is known about the role played by innate immunity to infection with this virus. We have investigated the occurrence, composition, activation status, and permissiveness to infection of peritoneal leukocytes (PLs) in Xenopus adults during FV3 infection by microscopy, flow cytometry, and reverse transcription-PCR. The total number of PLs and the relative fraction of activated mononucleated macrophage-like cells significantly increase as early as 1 day postinfection (dpi), followed by NK cells at 3 dpi, before the peak of the T-cell response at 6 dpi. FV3 infection also induces a rapid upregulation of proinflammatory genes including arginase 1, interleukin-1beta, and tumor necrosis factor alpha. Although PLs are susceptible to FV3 infection, as evidenced by apoptotic cells, active FV3 transcription, and the detection of viral particles by electron microscopy, the infection is weaker (fewer infectious particles), more transitory, and involves a smaller fraction (less than 1%) of PLs than the kidney, the main site of infection. However, viral DNA remains detectable in PLs for at least 3 weeks postinfection, past the point of viral clearance observed in the kidneys. This suggests that although PLs are actively involved in anti-FV3 immune responses, some of these cells can be permissive and harbor quiescent, asymptomatic FV3.
Chinchar,
Localization of frog virus 3 proteins using monoclonal antibodies.
1984, Pubmed
Chinchar,
Localization of frog virus 3 proteins using monoclonal antibodies.
1984,
Pubmed Chinchar,
Ranaviruses (family Iridoviridae): emerging cold-blooded killers.
2002,
Pubmed Chinchar,
Induction of apoptosis in frog virus 3-infected cells.
2003,
Pubmed Cunningham,
Emerging epidemic diseases of frogs in Britain are dependent on the source of ranavirus agent and the route of exposure.
2007,
Pubmed Daszak,
Emerging infectious diseases and amphibian population declines.
1999,
Pubmed De Zoysa,
First molluscan TNF-alpha homologue of the TNF superfamily in disk abalone: molecular characterization and expression analysis.
2009,
Pubmed Duffus,
Frog virus 3-like infections in aquatic amphibian communities.
2008,
Pubmed Ellermann-Eriksen,
Macrophages and cytokines in the early defence against herpes simplex virus.
2005,
Pubmed Flajnik,
Evolution of the MHC: antigenicity and unusual tissue distribution of Xenopus (frog) class II molecules.
1990,
Pubmed
,
Xenbase Fox,
First case of ranavirus-associated morbidity and mortality in natural populations of the South American frog Atelognathus patagonicus.
2006,
Pubmed Gantress,
Development and characterization of a model system to study amphibian immune responses to iridoviruses.
2003,
Pubmed
,
Xenbase Grayfer,
Molecular characterization of tumor necrosis factor receptors 1 and 2 of the goldfish (Carassius aurutus L.).
2009,
Pubmed Green,
Epizootiology of sixty-four amphibian morbidity and mortality events in the USA, 1996-2001.
2002,
Pubmed Greer,
Five amphibian mortality events associated with ranavirus infection in south central Ontario, Canada.
2005,
Pubmed Hadji-Azimi,
Atlas of adult Xenopus laevis laevis hematology.
1987,
Pubmed
,
Xenbase Hesse,
Differential regulation of nitric oxide synthase-2 and arginase-1 by type 1/type 2 cytokines in vivo: granulomatous pathology is shaped by the pattern of L-arginine metabolism.
2001,
Pubmed Horton,
Xenopus NK cells identified by novel monoclonal antibodies.
2000,
Pubmed
,
Xenbase Hsu,
Studies on Xenopus immunoglobulins using monoclonal antibodies.
1984,
Pubmed
,
Xenbase Hyatt,
Comparative studies of piscine and amphibian iridoviruses.
2000,
Pubmed Jürgens,
Identification of a candidate CD5 homologue in the amphibian Xenopus laevis.
1995,
Pubmed
,
Xenbase Lee,
Keeping NK cells in highly regulated antiviral warfare.
2007,
Pubmed Lin,
Macrophage-tropic HIV induces and exploits dendritic cell chemotaxis.
2000,
Pubmed Maniero,
Generation of a long-lasting, protective, and neutralizing antibody response to the ranavirus FV3 by the frog Xenopus.
2006,
Pubmed
,
Xenbase Marr,
CD91 up-regulates upon immune stimulation in Xenopus adult but not larval peritoneal leukocytes.
2005,
Pubmed
,
Xenbase Marr,
Localization and differential expression of activation-induced cytidine deaminase in the amphibian Xenopus upon antigen stimulation and during early development.
2007,
Pubmed
,
Xenbase Martinez,
Alternative activation of macrophages: an immunologic functional perspective.
2009,
Pubmed Mawaribuchi,
Tumor necrosis factor-alpha attenuates thyroid hormone-induced apoptosis in vascular endothelial cell line XLgoo established from Xenopus tadpole tails.
2008,
Pubmed
,
Xenbase Mendelson,
Factors associated with the catastrophic decline of a cloudforest frog fauna in Guatemala.
2004,
Pubmed Miller,
Concurrent infection with ranavirus, Batrachochytrium dendrobatidis, and Aeromonas in a captive anuran colony.
2008,
Pubmed Morales,
Characterization of primary and memory CD8 T-cell responses against ranavirus (FV3) in Xenopus laevis.
2007,
Pubmed
,
Xenbase Morales,
In vivo and in vitro techniques for comparative study of antiviral T-cell responses in the amphibian Xenopus.
2008,
Pubmed
,
Xenbase Munder,
Th1/Th2-regulated expression of arginase isoforms in murine macrophages and dendritic cells.
1999,
Pubmed Pesce,
Arginase-1-expressing macrophages suppress Th2 cytokine-driven inflammation and fibrosis.
2009,
Pubmed Rau,
Identification and characterization of Xenopus CD8+ T cells expressing an NK cell-associated molecule.
2002,
Pubmed
,
Xenbase Rigden,
Macrophage phagocytosis of foot-and-mouth disease virus may create infectious carriers.
2002,
Pubmed Robert,
Xenopus laevis: a possible vector of Ranavirus infection?
2007,
Pubmed
,
Xenbase Robert,
Xenopus, a unique comparative model to explore the role of certain heat shock proteins and non-classical MHC class Ib gene products in immune surveillance.
2009,
Pubmed
,
Xenbase Robert,
Adaptive immunity and histopathology in frog virus 3-infected Xenopus.
2005,
Pubmed
,
Xenbase Watkins,
A factor with interleukin-1-like activity is produced by peritoneal cells from the frog, Xenopus laevis.
1987,
Pubmed
,
Xenbase Way,
Persistent Ross River virus infection of murine macrophages: an in vitro model for the study of viral relapse and immune modulation during long-term infection.
2002,
Pubmed Weck,
Macrophages are the major reservoir of latent murine gammaherpesvirus 68 in peritoneal cells.
1999,
Pubmed Williams,
A decade of advances in iridovirus research.
2005,
Pubmed Zhang,
Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome.
2001,
Pubmed Zou,
Molecular cloning of the gene for interleukin-1beta from Xenopus laevis and analysis of expression in vivo and in vitro.
2000,
Pubmed
,
Xenbase Zupanovic,
Giant toads Bufo marinus in Australia and Venezuela have antibodies against 'ranaviruses'.
1998,
Pubmed
