Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2010 Feb 02;1075:1936-41. doi: 10.1073/pnas.0904497107.
Show Gene links
Show Anatomy links
Remosomes: RSC generated non-mobilized particles with approximately 180 bp DNA loosely associated with the histone octamer.
Shukla MS, Syed SH, Montel F, Faivre-Moskalenko C, Bednar J, Travers A, Angelov D, Dimitrov S.
???displayArticle.abstract???
Chromatin remodelers are sophisticated nano-machines that are able to alter histone-DNA interactions and to mobilize nucleosomes. Neither the mechanism of their action nor the conformation of the remodeled nucleosomes are, however, yet well understood. We have studied the mechanism of Remodels Structure of Chromatin (RSC)-nucleosome mobilization by using high-resolution microscopy and biochemical techniques. Atomic force microscopy and electron cryomicroscopy (EC-M) analyses show that two types of products are generated during the RSC remodeling: (i) stable non-mobilized particles, termed remosomes that contain about 180 bp of DNA associated with the histone octamer and, (ii) mobilized particles located at the end of DNA. EC-M reveals that individual remosomes exhibit a distinct, variable, highly-irregular DNA trajectory. The use of the unique "one pot assays" for studying the accessibility of nucleosomal DNA towards restriction enzymes, DNase I footprinting and ExoIII mapping demonstrate that the histone-DNA interactions within the remosomes are strongly perturbed, particularly in the vicinity of the nucleosome dyad. The data suggest a two-step mechanism of RSC-nucleosome remodeling consisting of an initial formation of a remosome followed by mobilization. In agreement with this model, we show experimentally that the remosomes are intermediate products generated during the first step of the remodeling reaction that are further efficiently mobilized by RSC.
Bao,
SnapShot: chromatin remodeling complexes.
2007,
Pubmed Bruno,
Histone H2A/H2B dimer exchange by ATP-dependent chromatin remodeling activities.
2003,
Pubmed
,
Xenbase Cairns,
RSC, an essential, abundant chromatin-remodeling complex.
1996,
Pubmed Chaban,
Structure of a RSC-nucleosome complex and insights into chromatin remodeling.
2008,
Pubmed Clapier,
Critical role for the histone H4 N terminus in nucleosome remodeling by ISWI.
2001,
Pubmed
,
Xenbase Côté,
Perturbation of nucleosome core structure by the SWI/SNF complex persists after its detachment, enhancing subsequent transcription factor binding.
1998,
Pubmed Dubochet,
Cryo-electron microscopy of vitrified specimens.
1988,
Pubmed Gangaraju,
Mechanisms of ATP dependent chromatin remodeling.
2007,
Pubmed Hamiche,
Histone tails modulate nucleosome mobility and regulate ATP-dependent nucleosome sliding by NURF.
2001,
Pubmed Havas,
Generation of superhelical torsion by ATP-dependent chromatin remodeling activities.
2000,
Pubmed
,
Xenbase Kassabov,
SWI/SNF unwraps, slides, and rewraps the nucleosome.
2003,
Pubmed
,
Xenbase Kwon,
Nucleosome disruption and enhancement of activator binding by a human SW1/SNF complex.
1994,
Pubmed
,
Xenbase Längst,
ISWI induces nucleosome sliding on nicked DNA.
2001,
Pubmed Längst,
Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer.
1999,
Pubmed Lia,
Direct observation of DNA distortion by the RSC complex.
2006,
Pubmed Lorch,
RSC unravels the nucleosome.
2001,
Pubmed
,
Xenbase Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed Mizuguchi,
ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex.
2004,
Pubmed Montel,
Atomic force microscopy imaging of SWI/SNF action: mapping the nucleosome remodeling and sliding.
2007,
Pubmed
,
Xenbase Saha,
Chromatin remodeling through directional DNA translocation from an internal nucleosomal site.
2005,
Pubmed
,
Xenbase Wu,
A 'one-pot' assay for the accessibility of DNA in a nucleosome core particle.
2004,
Pubmed Zhang,
DNA translocation and loop formation mechanism of chromatin remodeling by SWI/SNF and RSC.
2006,
Pubmed Zofall,
Chromatin remodeling by ISW2 and SWI/SNF requires DNA translocation inside the nucleosome.
2006,
Pubmed
