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Chromosome Res
2010 Jun 01;184:431-9. doi: 10.1007/s10577-010-9127-x.
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Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH.
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Laser microdissection was used for the preparation of whole chromosome painting probes in Silurana (Xenopus) tropicalis. Subsequent cross-species fluorescence in situ hybridization (Zoo-FISH) on its tetraploid relative Xenopus laevis revealed persistence of chromosomal quartets even after 50-65 million years of separate evolution. Their arrangement is in a partial concordance with previous experiments based on similarity of a high-resolution replication banding pattern. Further support for an allotetraploid origin of X. laevis was given by hybridization with a probe derived from the smallest X. tropicalis chromosome (Xt10). Here, pericentric areas of both arms of Xl 14 and 18 were stained, indicating intrachromosomal rearrangements. The positions of signals were not in agreement with the chromosomal quartets revealed by painting probes Xt 8 and 9 (Xl 11 + 14 and Xl 15 + 18, respectively). This suggests that both X. tropicalis chromosomes underwent non-reciprocal translocation of Xt10 separately in at least two different ancient ancestors. In addition, the observed translocation events could explain the origin of individuals with 18 chromosomes in diploid karyotypes, probably extinct after the genesis of the allotetraploid X. laevis (2n = 36).
Chowdhary,
Chromosome painting in farm, pet and wild animal species.
2001, Pubmed
Chowdhary,
Chromosome painting in farm, pet and wild animal species.
2001,
Pubmed Chowdhary,
HSA4 and GGA4: remarkable conservation despite 300-Myr divergence.
2000,
Pubmed Courtet,
Major histocompatibility complex and immunoglobulin loci visualized by in situ hybridization on Xenopus chromosomes.
2001,
Pubmed
,
Xenbase Evans,
A mitochondrial DNA phylogeny of African clawed frogs: phylogeography and implications for polyploid evolution.
2004,
Pubmed
,
Xenbase Evans,
Genome evolution and speciation genetics of clawed frogs (Xenopus and Silurana).
2008,
Pubmed
,
Xenbase Fujiwara,
Chromosomal localization and heterochromatin association of ribosomal RNA gene loci and silver-stained nucleolar organizer regions in salmonid fishes.
1998,
Pubmed Fuková,
Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin.
2007,
Pubmed Gribble,
Chromosome paints from single copies of chromosomes.
2004,
Pubmed Griffin,
Whole genome comparative studies between chicken and turkey and their implications for avian genome evolution.
2008,
Pubmed Guttenbach,
Comparative chromosome painting of chicken autosomal paints 1-9 in nine different bird species.
2003,
Pubmed Hellsten,
Accelerated gene evolution and subfunctionalization in the pseudotetraploid frog Xenopus laevis.
2007,
Pubmed
,
Xenbase Hotz,
Linkage groups of protein-coding genes in western palearctic water frogs reveal extensive evolutionary conservation.
1997,
Pubmed
,
Xenbase Khokha,
Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.
2009,
Pubmed
,
Xenbase Klein,
Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
2002,
Pubmed
,
Xenbase Krylov,
The c-SRC1 gene visualized by in situ hybridization on Xenopus laevis chromosomes.
2003,
Pubmed
,
Xenbase Krylov,
Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA.
2007,
Pubmed
,
Xenbase Kubickova,
The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals.
2002,
Pubmed Ocalewicz,
Variation in size and location of the Ag-NOR in the Atlantic halibut (Hippoglossus hippoglossus).
2008,
Pubmed Pollet,
Insights from Xenopus genomes.
2006,
Pubmed
,
Xenbase Ráb,
Chromosome studies of European cyprinid fishes: interspecific homology of leuciscine cytotaxonomic marker-the largest subtelocentric chromosome pair as revealed by cross-species painting.
2008,
Pubmed Schmid,
Chromosome banding in Amphibia. XVI. High-resolution replication banding patterns in Xenopus laevis.
1991,
Pubmed
,
Xenbase Schmid,
Sex chromosomes, sex-linked genes, and sex determination in the vertebrate class amphibia.
2001,
Pubmed Schmid,
Chromosome banding in Amphibia. IX. The polyploid karyotypes of Odontophrynus americanus and Ceratophrys ornata (Anura, Leptodactylidae).
1985,
Pubmed Shetty,
Comparative painting reveals strong chromosome homology over 80 million years of bird evolution.
1999,
Pubmed Smith,
Gene order data from a model amphibian (Ambystoma): new perspectives on vertebrate genome structure and evolution.
2006,
Pubmed Teruel,
Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria.
2009,
Pubmed Tlapakova,
Localization, structure and polymorphism of two paralogous Xenopus laevis mitochondrial malate dehydrogenase genes.
2005,
Pubmed
,
Xenbase Tymowska,
Karyotype analysis of Xenopus tropicalis Gray, Pipidae.
1973,
Pubmed
,
Xenbase Tymowska,
A comparison of the karyotype, constitutive heterochromatin, and nucleolar organizer regions of the new tetraploid species Xenopus epitropicalis Fischberg and Picard with those of Xenopus tropicalis Gray (Anura, Pipidae).
1982,
Pubmed
,
Xenbase Tymowska,
Karyotype analysis of Xenopus muelleri (Peters) and Xenopus laevis (Daudin), Pipidae.
1972,
Pubmed
,
Xenbase Vítková,
Molecular divergence of the W chromosomes in pyralid moths (Lepidoptera).
2007,
Pubmed
