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Proc Natl Acad Sci U S A
2010 May 04;10718:8440-5. doi: 10.1073/pnas.1001425107.
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Drosophila TRPA1 channel mediates chemical avoidance in gustatory receptor neurons.
Kim SH, Lee Y, Akitake B, Woodward OM, Guggino WB, Montell C.
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Mammalian sweet, bitter, and umami taste is mediated by a single transduction pathway that includes a phospholipase C (PLC)beta and one cation channel, TRPM5. However, in insects such as the fruit fly, Drosophila melanogaster, it is unclear whether different tastants, such as bitter compounds, are sensed in gustatory receptor neurons (GRNs) through one or multiple ion channels, as the cation channels required in insect GRNs are unknown. Here, we set out to explore additional sensory roles for the Drosophila TRPA1 channel, which was known to function in thermosensation. We found that TRPA1 was expressed in GRNs that respond to aversive compounds. Elimination of TRPA1 had no impact on the responses to nearly all bitter compounds tested, including caffeine, quinine, and strychnine. Rather, we found that TRPA1 was required in a subset of avoidance GRNs for the behavioral and electrophysiological responses to aristolochic acid. TRPA1 did not appear to be activated or inhibited directly by aristolochic acid. We found that elimination of the same PLC that leads to activation of TRPA1 in thermosensory neurons was also required in the TRPA1-expressing GRNs for avoiding aristolochic acid. Given that mammalian TRPA1 is required for responding to noxious chemicals, many of which cause pain and injury, our analysis underscores the evolutionarily conserved role for TRPA1 channels in chemical avoidance.
Al-Anzi,
Response of Drosophila to wasabi is mediated by painless, the fly homolog of mammalian TRPA1/ANKTM1.
2006, Pubmed
Al-Anzi,
Response of Drosophila to wasabi is mediated by painless, the fly homolog of mammalian TRPA1/ANKTM1.
2006,
Pubmed Bandell,
Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin.
2004,
Pubmed Bautista,
TRPA1 mediates the inflammatory actions of environmental irritants and proalgesic agents.
2006,
Pubmed Bautista,
Pungent products from garlic activate the sensory ion channel TRPA1.
2005,
Pubmed Caterina,
Impaired nociception and pain sensation in mice lacking the capsaicin receptor.
2000,
Pubmed Dahanukar,
A Gr receptor is required for response to the sugar trehalose in taste neurons of Drosophila.
2001,
Pubmed Dahanukar,
Two Gr genes underlie sugar reception in Drosophila.
2007,
Pubmed Davis,
Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesia.
2000,
Pubmed Dunipace,
Spatially restricted expression of candidate taste receptors in the Drosophila gustatory system.
2001,
Pubmed Glendinning,
Electrophysiological evidence for two transduction pathways within a bitter-sensitive taste receptor.
1997,
Pubmed Glendinning,
Contribution of different taste cells and signaling pathways to the discrimination of "bitter" taste stimuli by an insect.
2002,
Pubmed Gong,
Ends-out, or replacement, gene targeting in Drosophila.
2003,
Pubmed Hamada,
An internal thermal sensor controlling temperature preference in Drosophila.
2008,
Pubmed
,
Xenbase Hiroi,
Differentiated response to sugars among labellar chemosensilla in Drosophila.
2002,
Pubmed Jiao,
A Drosophila gustatory receptor required for the responses to sucrose, glucose, and maltose identified by mRNA tagging.
2007,
Pubmed Jiao,
Gr64f is required in combination with other gustatory receptors for sugar detection in Drosophila.
2008,
Pubmed Jordt,
Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1.
2004,
Pubmed Karashima,
TRPA1 acts as a cold sensor in vitro and in vivo.
2009,
Pubmed Kim,
A TRPV family ion channel required for hearing in Drosophila.
2003,
Pubmed Kindt,
Caenorhabditis elegans TRPA-1 functions in mechanosensation.
2007,
Pubmed Kwan,
TRPA1 contributes to cold, mechanical, and chemical nociception but is not essential for hair-cell transduction.
2006,
Pubmed Kwon,
Control of thermotactic behavior via coupling of a TRP channel to a phospholipase C signaling cascade.
2008,
Pubmed Lee,
Multiple gustatory receptors required for the caffeine response in Drosophila.
2009,
Pubmed Macpherson,
The pungency of garlic: activation of TRPA1 and TRPV1 in response to allicin.
2005,
Pubmed Meunier,
Peripheral coding of bitter taste in Drosophila.
2003,
Pubmed Montell,
Drosophila TRP channels.
2005,
Pubmed Montell,
A taste of the Drosophila gustatory receptors.
2009,
Pubmed Moon,
A Drosophila gustatory receptor essential for aversive taste and inhibiting male-to-male courtship.
2009,
Pubmed Moon,
A taste receptor required for the caffeine response in vivo.
2006,
Pubmed Niemeyer,
The Drosophila light-activated conductance is composed of the two channels TRP and TRPL.
1996,
Pubmed Obata,
TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury.
2005,
Pubmed Pearn,
Molecular, biochemical, and electrophysiological characterization of Drosophila norpA mutants.
1996,
Pubmed Pérez,
A transient receptor potential channel expressed in taste receptor cells.
2002,
Pubmed
,
Xenbase Prober,
Zebrafish TRPA1 channels are required for chemosensation but not for thermosensation or mechanosensory hair cell function.
2008,
Pubmed
,
Xenbase Rosenzweig,
The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis.
2005,
Pubmed Slone,
Sugar receptors in Drosophila.
2007,
Pubmed Story,
ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures.
2003,
Pubmed Talavera,
Heat activation of TRPM5 underlies thermal sensitivity of sweet taste.
2005,
Pubmed Talavera,
Nicotine activates the chemosensory cation channel TRPA1.
2009,
Pubmed Thorne,
Taste perception and coding in Drosophila.
2004,
Pubmed Tracey,
painless, a Drosophila gene essential for nociception.
2003,
Pubmed Ueno,
Trehalose sensitivity in Drosophila correlates with mutations in and expression of the gustatory receptor gene Gr5a.
2001,
Pubmed van der Goes van Naters,
Insects as chemosensors of humans and crops.
2006,
Pubmed Venkatachalam,
TRP channels.
2007,
Pubmed Viswanath,
Opposite thermosensor in fruitfly and mouse.
2003,
Pubmed Vosshall,
Molecular architecture of smell and taste in Drosophila.
2007,
Pubmed Wang,
Dissecting independent channel and scaffolding roles of the Drosophila transient receptor potential channel.
2005,
Pubmed Wang,
Taste representations in the Drosophila brain.
2004,
Pubmed Yoon,
Specific molecular alterations in the norpA-encoded phospholipase C of Drosophila and their effects on electrophysiological responses in vivo.
2004,
Pubmed Zhang,
Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways.
2003,
Pubmed
