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In metazoa, nuclear pore complexes (NPCs) assemble from disassembled precursors into a reforming nuclear envelope (NE) at the end of mitosis and into growing intact NEs during interphase. Here, we show via RNAi-mediated knockdown that ELYS, a nucleoporin critical for the recruitment of the essential Nup107/160 complex to chromatin, is required for NPC assembly at the end of mitosis but not during interphase. Conversely, the transmembrane nucleoporin POM121 is critical for the incorporation of the Nup107/160 complex into new assembly sites specifically during interphase. Strikingly, recruitment of the Nup107/160 complex to an intact NE involves a membrane curvature-sensing domain of its constituent Nup133, which is not required for postmitotic NPC formation. Our results suggest that in organisms with open mitosis, NPCs assemble via two distinct mechanisms to accommodate cell cycle-dependent differences in NE topology.
Alber,
The molecular architecture of the nuclear pore complex.
2007, Pubmed
Alber,
The molecular architecture of the nuclear pore complex.
2007,
Pubmed Anderson,
The life cycle of the metazoan nuclear envelope.
2008,
Pubmed Anderson,
Recruitment of functionally distinct membrane proteins to chromatin mediates nuclear envelope formation in vivo.
2009,
Pubmed Anderson,
Reshaping of the endoplasmic reticulum limits the rate for nuclear envelope formation.
2008,
Pubmed Anderson,
Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum.
2007,
Pubmed
,
Xenbase Antonin,
The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.
2005,
Pubmed
,
Xenbase Antonin,
Nuclear pore complex assembly through the cell cycle: regulation and membrane organization.
2008,
Pubmed Antonin,
Nuclear envelope: membrane bending for pore formation?
2009,
Pubmed Antonny,
Membrane deformation by protein coats.
2006,
Pubmed Beck,
Nuclear pore complex structure and dynamics revealed by cryoelectron tomography.
2004,
Pubmed Belgareh,
An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells.
2001,
Pubmed Berke,
Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex.
2004,
Pubmed Boehmer,
Structural and functional studies of Nup107/Nup133 interaction and its implications for the architecture of the nuclear pore complex.
2008,
Pubmed Brohawn,
Structural evidence for common ancestry of the nuclear pore complex and vesicle coats.
2008,
Pubmed Brohawn,
The nuclear pore complex has entered the atomic age.
2009,
Pubmed Chial,
Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies.
1998,
Pubmed Cronshaw,
Proteomic analysis of the mammalian nuclear pore complex.
2002,
Pubmed D'Angelo,
Age-dependent deterioration of nuclear pore complexes causes a loss of nuclear integrity in postmitotic cells.
2009,
Pubmed D'Angelo,
Nuclear pores form de novo from both sides of the nuclear envelope.
2006,
Pubmed
,
Xenbase Davis,
Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway.
1987,
Pubmed Dawson,
ER membrane-bending proteins are necessary for de novo nuclear pore formation.
2009,
Pubmed
,
Xenbase Debler,
A fence-like coat for the nuclear pore membrane.
2008,
Pubmed De Craene,
Rtn1p is involved in structuring the cortical endoplasmic reticulum.
2006,
Pubmed Devos,
Components of coated vesicles and nuclear pore complexes share a common molecular architecture.
2004,
Pubmed Drin,
A general amphipathic alpha-helical motif for sensing membrane curvature.
2007,
Pubmed Dultz,
Systematic kinetic analysis of mitotic dis- and reassembly of the nuclear pore in living cells.
2008,
Pubmed Eriksson,
Dynamic properties of nuclear pore complex proteins in gp210 deficient cells.
2004,
Pubmed Franz,
MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly.
2007,
Pubmed
,
Xenbase Gillespie,
ELYS/MEL-28 chromatin association coordinates nuclear pore complex assembly and replication licensing.
2007,
Pubmed
,
Xenbase Güttinger,
Orchestrating nuclear envelope disassembly and reassembly during mitosis.
2009,
Pubmed Hallberg,
An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region.
1993,
Pubmed Harel,
Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.
2003,
Pubmed
,
Xenbase Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase Hetzer,
Pushing the envelope: structure, function, and dynamics of the nuclear periphery.
2005,
Pubmed King,
Karyopherin-mediated import of integral inner nuclear membrane proteins.
2006,
Pubmed Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase Lu,
Cisternal organization of the endoplasmic reticulum during mitosis.
2009,
Pubmed Lupu,
Nuclear pore composition regulates neural stem/progenitor cell differentiation in the mouse embryo.
2008,
Pubmed Mansfeld,
The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells.
2006,
Pubmed
,
Xenbase Maul,
On the octagonality of the nuclear pore complex.
1971,
Pubmed Rabut,
Mapping the dynamic organization of the nuclear pore complex inside single living cells.
2004,
Pubmed Rasala,
ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division.
2006,
Pubmed
,
Xenbase Rasala,
Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.
2008,
Pubmed
,
Xenbase Schulte,
Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions.
2008,
Pubmed
,
Xenbase Stavru,
Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells.
2006,
Pubmed Stavru,
NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes.
2006,
Pubmed Voeltz,
A class of membrane proteins shaping the tubular endoplasmic reticulum.
2006,
Pubmed Walker,
Nuclear envelope: Different preNup arrangements.
2010,
Pubmed Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase Walther,
RanGTP mediates nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase Weis,
Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle.
2003,
Pubmed
