Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Org Lett
2010 Sep 03;1217:3776-9. doi: 10.1021/ol101408f.
Show Gene links
Show Anatomy links
Preparation of translationally competent tRNA by direct chemical acylation.
Duffy NH, Dougherty DA.
???displayArticle.abstract???
Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) using amino acid phosphate esters. The successful implementation of this methodology to full-length suppressor tRNA is described, and it is shown that the derived aminoacyl-tRNA is translationally competent in Xenopus oocytes.
???displayArticle.pubmedLink???
20687605 ???displayArticle.pmcLink???PMC2929286 ???displayArticle.link???Org Lett ???displayArticle.grants???[+]
Arnez,
Structural and functional considerations of the aminoacylation reaction.
1997, Pubmed
Arnez,
Structural and functional considerations of the aminoacylation reaction.
1997,
Pubmed Caravan,
Gadolinium(III) Chelates as MRI Contrast Agents: Structure, Dynamics, and Applications.
1999,
Pubmed Dougherty,
Unnatural amino acids as probes of protein structure and function.
2000,
Pubmed Dougherty,
Cys-loop neuroreceptors: structure to the rescue?
2008,
Pubmed Link,
Reassignment of sense codons in vivo.
2005,
Pubmed Nowak,
In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system.
1998,
Pubmed
,
Xenbase Ohuchi,
The flexizyme system: a highly flexible tRNA aminoacylation tool for the translation apparatus.
2007,
Pubmed Parker,
Being excited by lanthanide coordination complexes: aqua species, chirality, excited-state chemistry, and exchange dynamics.
2002,
Pubmed Petersson,
MALDI-TOF mass spectrometry methods for evaluation of in vitro aminoacyl tRNA production.
2002,
Pubmed Rodriguez,
Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 1: minimizing misacylation.
2007,
Pubmed
,
Xenbase Rodriguez,
Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 2: evaluating suppression efficiency.
2007,
Pubmed
,
Xenbase Saks,
An engineered Tetrahymena tRNAGln for in vivo incorporation of unnatural amino acids into proteins by nonsense suppression.
1996,
Pubmed
,
Xenbase Tzvetkova,
Biomimetic aminoacylation of ribonucleotides and RNA with aminoacyl phosphate esters and lanthanum salts.
2007,
Pubmed Xiu,
Nicotine binding to brain receptors requires a strong cation-pi interaction.
2009,
Pubmed
,
Xenbase Young,
Beyond the canonical 20 amino acids: expanding the genetic lexicon.
2010,
Pubmed Zhong,
From ab initio quantum mechanics to molecular neurobiology: a cation-pi binding site in the nicotinic receptor.
1998,
Pubmed
