Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
SUMO conjugation of cellular proteins is essential for proper progression of mitosis. PIASy, a SUMO E3 ligase, is required for mitotic SUMOylation of chromosomal proteins, yet the regulatory mechanism behind the PIASy-dependent SUMOylation during mitosis has not been determined. Using a series of truncated PIASy proteins, we have found that the N terminus of PIASy is not required for SUMO modification in vitro but is essential for mitotic SUMOylation in Xenopus egg extracts. We demonstrate that swapping the N terminus of PIASy protein with the corresponding region of other PIAS family members abolishes chromosomal binding and mitotic SUMOylation. We further show that the N-terminal domain of PIASy is sufficient for centromeric localization. We identified that the N-terminal domain of PIASy interacts with the Rod/Zw10 complex, and immunofluorescence further reveals that PIASy colocalizes with Rod/Zw10 in the centromeric region. We show that the Rod/Zw10 complex interacts with the first 47 residues of PIASy which were particularly important for mitotic SUMOylation. Finally, we show that depletion of Rod compromises the centromeric localization of PIASy and SUMO2/3 in mitosis. Together, we demonstrate a fundamental mechanism of PIASy to localize in the centromeric region of chromosome to execute centromeric SUMOylation during mitosis.
al-Khodairy,
The Schizosaccharomyces pombe hus5 gene encodes a ubiquitin conjugating enzyme required for normal mitosis.
1995, Pubmed
al-Khodairy,
The Schizosaccharomyces pombe hus5 gene encodes a ubiquitin conjugating enzyme required for normal mitosis.
1995,
Pubmed Aravind,
SAP - a putative DNA-binding motif involved in chromosomal organization.
2000,
Pubmed Azuma,
SUMO-2/3 regulates topoisomerase II in mitosis.
2003,
Pubmed
,
Xenbase Azuma,
Analysis of SUMOylation of topoisomerase IIalpha with Xenopus egg extracts.
2009,
Pubmed
,
Xenbase Azuma,
PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomes.
2005,
Pubmed
,
Xenbase Azuma,
Expression and regulation of the mammalian SUMO-1 E1 enzyme.
2001,
Pubmed Basto,
In vivo dynamics of the rough deal checkpoint protein during Drosophila mitosis.
2004,
Pubmed Basto,
Rough deal and Zw10 are required for the metaphase checkpoint in Drosophila.
2000,
Pubmed Bernier-Villamor,
Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1.
2002,
Pubmed Biggins,
Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae.
2001,
Pubmed Bossis,
SUMO: regulating the regulator.
2006,
Pubmed Buffin,
Recruitment of Mad2 to the kinetochore requires the Rod/Zw10 complex.
2005,
Pubmed Dasso,
Emerging roles of the SUMO pathway in mitosis.
2008,
Pubmed Dawlaty,
Resolution of sister centromeres requires RanBP2-mediated SUMOylation of topoisomerase IIalpha.
2008,
Pubmed Duval,
The 'PINIT' motif, of a newly identified conserved domain of the PIAS protein family, is essential for nuclear retention of PIAS3L.
2003,
Pubmed Hay,
SUMO: a history of modification.
2005,
Pubmed Hecker,
Specification of SUMO1- and SUMO2-interacting motifs.
2006,
Pubmed Johnson,
An E3-like factor that promotes SUMO conjugation to the yeast septins.
2001,
Pubmed Johnson,
Protein modification by SUMO.
2004,
Pubmed Karess,
Rod-Zw10-Zwilch: a key player in the spindle checkpoint.
2005,
Pubmed Kops,
ZW10 links mitotic checkpoint signaling to the structural kinetochore.
2005,
Pubmed
,
Xenbase Kotaja,
PIAS proteins modulate transcription factors by functioning as SUMO-1 ligases.
2002,
Pubmed Lee,
PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein.
2006,
Pubmed Lu,
The RZZ complex and the spindle assembly checkpoint.
2009,
Pubmed Okubo,
NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers.
2004,
Pubmed Palvimo,
PIAS proteins as regulators of small ubiquitin-related modifier (SUMO) modifications and transcription.
2007,
Pubmed Pichler,
The RanBP2 SUMO E3 ligase is neither HECT- nor RING-type.
2004,
Pubmed Pichler,
The nucleoporin RanBP2 has SUMO1 E3 ligase activity.
2002,
Pubmed Reindle,
Multiple domains in Siz SUMO ligases contribute to substrate selectivity.
2006,
Pubmed Rytinki,
PIAS proteins: pleiotropic interactors associated with SUMO.
2009,
Pubmed Ryu,
PIASy mediates SUMO-2/3 conjugation of poly(ADP-ribose) polymerase 1 (PARP1) on mitotic chromosomes.
2010,
Pubmed
,
Xenbase Santaguida,
The life and miracles of kinetochores.
2009,
Pubmed Scaërou,
The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore.
2001,
Pubmed Scaërou,
The rough deal protein is a new kinetochore component required for accurate chromosome segregation in Drosophila.
1999,
Pubmed Sekiyama,
Structure of the small ubiquitin-like modifier (SUMO)-interacting motif of MBD1-containing chromatin-associated factor 1 bound to SUMO-3.
2008,
Pubmed Shayeghi,
Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance.
1997,
Pubmed Starr,
ZW10 helps recruit dynactin and dynein to the kinetochore.
1998,
Pubmed Suzuki,
Solution structures and DNA binding properties of the N-terminal SAP domains of SUMO E3 ligases from Saccharomyces cerevisiae and Oryza sativa.
2009,
Pubmed Takahashi,
Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro.
2003,
Pubmed Tanaka,
Characterization of a fission yeast SUMO-1 homologue, pmt3p, required for multiple nuclear events, including the control of telomere length and chromosome segregation.
1999,
Pubmed Tolkunova,
PIAS proteins as repressors of Oct4 function.
2007,
Pubmed Torras-Llort,
Focus on the centre: the role of chromatin on the regulation of centromere identity and function.
2009,
Pubmed Wang,
Three classes of genes mutated in colorectal cancers with chromosomal instability.
2004,
Pubmed Williams,
Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions.
2003,
Pubmed Yunus,
Structure of the Siz/PIAS SUMO E3 ligase Siz1 and determinants required for SUMO modification of PCNA.
2009,
Pubmed Yunus,
Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway.
2006,
Pubmed
