Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Toxicol Appl Pharmacol
2011 Jan 15;2502:170-83. doi: 10.1016/j.taap.2010.10.010.
Show Gene links
Show Anatomy links
New CYP1 genes in the frog Xenopus (Silurana) tropicalis: induction patterns and effects of AHR agonists during development.
Jönsson ME, Berg C, Goldstone JV, Stegeman JJ.
???displayArticle.abstract???
The Xenopus tropicalis genome shows a single gene in each of the four cytochrome P450 1 (CYP1) subfamilies that occur in vertebrates, designated as CYP1A, CYP1B1, CYP1C1, and CYP1D1. We cloned the cDNAs of these genes and examined their expression in untreated tadpoles and in tadpoles exposed to waterborne aryl hydrocarbon receptor agonists, 3,3',4,4',5-pentachlorobiphenyl (PCB126), β-naphthoflavone (βNF), or indigo. We also examined the effects of PCB126 on expression of genes involved in stress response, cell proliferation, thyroid homeostasis, and prostaglandin synthesis. PCB126 induced CYP1A, CYP1B1, and CYP1C1 but had little effect on CYP1D1 (77-, 1.7-, 4.6- and 1.4-fold induction versus the control, respectively). βNF induced CYP1A and CYP1C1 (26- and 2.5-fold), while, under conditions used, indigo tended to induce only CYP1A (1.9-fold). The extent of CYP1 induction by PCB126 and βNF was positively correlated to the number of putative dioxin response elements 0-20 kb upstream of the start codons. No morphological effect was observed in tadpoles exposed to 1 nM-10 μM PCB126 at two days post-fertilization (dpf) and screened 20 days later. However, in 14-dpf tadpoles a slight up-regulation of the genes for PCNA, transthyretin, HSC70, Cu-Zn SOD, and Cox-2 was observed two days after exposure to 1 μM PCB126. This study of the full suite of CYP1 genes in an amphibian species reveals gene- and AHR agonist-specific differences in response, as well as a much lower sensitivity to CYP1 induction and short-term toxicity by PCB126 compared with in fish larvae. The single genes in each CYP1 subfamily may make X. tropicalis a useful model for mechanistic studies of CYP1 functions.
Adachi,
Indirubin and indigo are potent aryl hydrocarbon receptor ligands present in human urine.
2001, Pubmed
Adachi,
Indirubin and indigo are potent aryl hydrocarbon receptor ligands present in human urine.
2001,
Pubmed Beatty,
Toxicity of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin in larval and adult forms of Rana catesbeiana.
1976,
Pubmed Binder,
Microsomal electron transport and xenobiotic monooxygenase activities during the embryonic period of development in the killifish, Fundulus heteroclitus.
1984,
Pubmed Brouwer,
Interactions of persistent environmental organohalogens with the thyroid hormone system: mechanisms and possible consequences for animal and human health.
1998,
Pubmed Brunström,
Differences between chick and turkey embryos in sensitivity to 3,3',4,4'-tetrachloro-biphenyl and in concentration/affinity of the hepatic receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin.
1988,
Pubmed Bugiak,
Hepatic and vascular mRNA expression in adult zebrafish (Danio rerio) following exposure to benzo-a-pyrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin.
2009,
Pubmed Carney,
Aryl hydrocarbon receptor activation produces heart-specific transcriptional and toxic responses in developing zebrafish.
2006,
Pubmed Chambers,
RALDH-independent generation of retinoic acid during vertebrate embryogenesis by CYP1B1.
2007,
Pubmed Cho,
Transcriptional activation of the human Cu/Zn superoxide dismutase gene by 2,3,7,8-tetrachlorodibenzo-p-dioxin through the xenobiotic-responsive element.
2001,
Pubmed Choudhary,
Cyp1b1 protein in the mouse eye during development: an immunohistochemical study.
2007,
Pubmed Dimova,
The E2F transcriptional network: old acquaintances with new faces.
2005,
Pubmed Dong,
2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity in the zebrafish embryo: local circulation failure in the dorsal midbrain is associated with increased apoptosis.
2002,
Pubmed Flaveny,
Ligand selectivity and gene regulation by the human aryl hydrocarbon receptor in transgenic mice.
2009,
Pubmed Fontenot,
Bioaccumulation of polychlorinated biphenyls in ranid frogs and northern water snakes from a hazardous waste site and a contaminated watershed.
2000,
Pubmed Fujita,
Molecular cloning and sequence analysis of cDNAs coding for 3-methylcholanthrene-inducible cytochromes P450 in Xenopus laevis liver.
1999,
Pubmed
,
Xenbase Gierthy,
Reversible inhibition of in vitro epithelial cell proliferation by 2,3,7,8-tetrachlorodibenzo-p-dioxin.
1984,
Pubmed Goldstone,
A revised evolutionary history of the CYP1A subfamily: gene duplication, gene conversion, and positive selection.
2006,
Pubmed Goldstone,
Cytochrome P450 1D1: a novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD.
2009,
Pubmed Goldstone,
Cytochrome P450 1 genes in early deuterostomes (tunicates and sea urchins) and vertebrates (chicken and frog): origin and diversification of the CYP1 gene family.
2007,
Pubmed
,
Xenbase Goldstone,
Gene structure of the novel cytochrome P4501D1 genes in stickleback (Gasterosteus aculeatus) and medaka (Oryzias latipes).
2008,
Pubmed Goleman,
Environmentally relevant concentrations of ammonium perchlorate inhibit thyroid function and alter sex ratios in developing Xenopus laevis.
2002,
Pubmed
,
Xenbase Guiney,
Hemodynamic dysfunction and cytochrome P4501A mRNA expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin during embryonic stages of lake trout development.
2000,
Pubmed Gyllenhammar,
Reproductive toxicity in Xenopus tropicalis after developmental exposure to environmental concentrations of ethynylestradiol.
2009,
Pubmed
,
Xenbase Hayes,
Hermaphroditic, demasculinized frogs after exposure to the herbicide atrazine at low ecologically relevant doses.
2002,
Pubmed
,
Xenbase Huang,
Exposure of northern leopard frogs in the green bay ecosystem to polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins, and polychlorinated dibenzofurans is measured by direct chemistry but not hepatic ethoxyresorufin-o-deethylase activity.
1999,
Pubmed Huang,
Multiple mechanisms are involved in Ah receptor-mediated cell cycle arrest.
2005,
Pubmed Huang,
Immunohistochemical localization of cytochrome P4501A induced by 3,3',4,4',5-pentachlorobiphenyl (PCB 126) in multiple organs of northern leopard frogs, Rana pipiens.
2001,
Pubmed Jönsson,
Role of AHR2 in the expression of novel cytochrome P450 1 family genes, cell cycle genes, and morphological defects in developing zebra fish exposed to 3,3',4,4',5-pentachlorobiphenyl or 2,3,7,8-tetrachlorodibenzo-p-dioxin.
2007,
Pubmed Jönsson,
Basal and 3,3',4,4',5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish.
2007,
Pubmed Jönsson,
The tryptophan photoproduct 6-formylindolo[3,2-b]carbazole (FICZ) binds multiple AHRs and induces multiple CYP1 genes via AHR2 in zebrafish.
2009,
Pubmed Jönsson,
Induction patterns of new CYP1 genes in environmentally exposed rainbow trout.
2010,
Pubmed Jönsson,
Cytochrome P4501A induction in rainbow trout gills and liver following exposure to waterborne indigo, benzo[a]pyrene and 3,3',4,4',5-pentachlorobiphenyl.
2006,
Pubmed Karchner,
The molecular basis for differential dioxin sensitivity in birds: role of the aryl hydrocarbon receptor.
2006,
Pubmed Laub,
Responsiveness of a Xenopus laevis cell line to the aryl hydrocarbon receptor ligands 6-formylindolo[3,2-b]carbazole (FICZ) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
2010,
Pubmed
,
Xenbase Lavine,
Aryl hydrocarbon receptors in the frog Xenopus laevis: two AhR1 paralogs exhibit low affinity for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
2005,
Pubmed
,
Xenbase Lehigh Shirey,
Polychlorinated biphenyl exposure delays metamorphosis and alters thyroid hormone system gene expression in developing Xenopus laevis.
2006,
Pubmed
,
Xenbase Leney,
Metamorphosis increases biotransformation of polychlorinated biphenyls: a comparative study of polychlorinated biphenyl metabolism in green frogs (Rana clamitans) and leopard frogs (Rana pipiens) at various life stages.
2006,
Pubmed Lewis,
Molecular modelling of human CYP1B1 substrate interactions and investigation of allelic variant effects on metabolism.
2003,
Pubmed Miyazaki,
Identification of the functional domain of thyroid hormone receptor responsible for polychlorinated biphenyl-mediated suppression of its action in vitro.
2008,
Pubmed Na,
Protective effects of vitamin E against 3,3',4,4',5-pentachlorobiphenyl (PCB126) induced toxicity in zebrafish embryos.
2009,
Pubmed Nukaya,
The role of the dioxin-responsive element cluster between the Cyp1a1 and Cyp1a2 loci in aryl hydrocarbon receptor biology.
2009,
Pubmed Ohi,
Molecular cloning and expression analysis of the aryl hydrocarbon receptor of Xenopus laevis.
2003,
Pubmed
,
Xenbase Pettersson,
Environmentally relevant concentrations of ethynylestradiol cause female-biased sex ratios in Xenopus tropicalis and Rana temporaria.
2007,
Pubmed
,
Xenbase Philips,
Developmental differences in elimination of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) during Xenopus laevis development.
2006,
Pubmed
,
Xenbase Puga,
Sustained increase in intracellular free calcium and activation of cyclooxygenase-2 expression in mouse hepatoma cells treated with dioxin.
1997,
Pubmed Rankouhi,
Induction of ethoxy-resorufin-O-deethylase activity by halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons in primary hepatocytes of the green frog (Rana esculenta).
2005,
Pubmed Rannug,
Certain photooxidized derivatives of tryptophan bind with very high affinity to the Ah receptor and are likely to be endogenous signal substances.
1987,
Pubmed Rowatt,
ARNT gene multiplicity in amphibians: characterization of ARNT2 from the frog Xenopus laevis.
2003,
Pubmed
,
Xenbase Ruijter,
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data.
2009,
Pubmed Schlezinger,
Uncoupling of cytochrome P450 1A and stimulation of reactive oxygen species production by co-planar polychlorinated biphenyl congeners.
2006,
Pubmed Schmittgen,
Analyzing real-time PCR data by the comparative C(T) method.
2008,
Pubmed Scornaienchi,
Functional differences in the cytochrome P450 1 family enzymes from zebrafish (Danio rerio) using heterologously expressed proteins.
2010,
Pubmed Smolowitz,
Immunohistochemical localization of cytochrome P-450IA1 induced by 3,3',4,4'-tetrachlorobiphenyl and by 2,3,7,8-tetrachlorodibenzoafuran in liver and extrahepatic tissues of the teleost Stenotomus chrysops (scup).
1991,
Pubmed Stamatakis,
RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models.
2006,
Pubmed Stuart,
Status and trends of amphibian declines and extinctions worldwide.
2004,
Pubmed Sugihara,
Comparative study of aryl hydrocarbon receptor ligand activities of six chemicals in vitro and in vivo.
2008,
Pubmed Sutter,
Complete cDNA sequence of a human dioxin-inducible mRNA identifies a new gene subfamily of cytochrome P450 that maps to chromosome 2.
1994,
Pubmed Ter Schure,
Latitudinal fractionation of polybrominated diphenyl ethers and polychlorinated biphenyls in frogs (Rana temporaria).
2002,
Pubmed Verslycke,
Isolation and phylogeny of novel cytochrome P450 genes from tunicates (Ciona spp.): a CYP3 line in early deuterostomes?
2006,
Pubmed Watabe,
Aryl hydrocarbon receptor functions as a potent coactivator of E2F1-dependent trascription activity.
2010,
Pubmed Whitlock,
Induction of cytochrome P4501A1.
1999,
Pubmed Yin,
Influence of TCDD on zebrafish CYP1B1 transcription during development.
2008,
Pubmed Zanette,
New cytochrome P450 1B1, 1C2 and 1D1 genes in the killifish Fundulus heteroclitus: Basal expression and response of five killifish CYP1s to the AHR agonist PCB126.
2009,
Pubmed Zeruth,
Functional analysis of cis-regulatory regions within the dioxin-inducible CYP1A promoter/enhancer region from zebrafish (Danio rerio).
2007,
Pubmed Zimmermann,
An aryl hydrocarbon receptor repressor from Xenopus laevis: function, expression, and role in dioxin responsiveness during frog development.
2008,
Pubmed
,
Xenbase
