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Cytokinesis and ciliogenesis are fundamental cellular processes that require strict coordination of microtubule organization and directed membrane trafficking. These processes have been intensely studied, but there has been little indication that regulatory machinery might be extensively shared between them. Here, we show that several central spindle/midbody proteins (PRC1, MKLP-1, INCENP, centriolin) also localize in specific patterns at the basal body complex in vertebrate ciliated epithelial cells. Moreover, bioinformatic comparisons of midbody and cilia proteomes reveal a highly significant degree of overlap. Finally, we used temperature-sensitive alleles of PRC1/spd-1 and MKLP-1/zen-4 in C. elegans to assess ciliary functions while bypassing these proteins' early role in cell division. These mutants displayed defects in both cilia function and cilia morphology. Together, these data suggest the conserved reuse of a surprisingly large number of proteins in the cytokinetic apparatus and in cilia.
Albertson,
Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle.
2008, Pubmed
Albertson,
Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle.
2008,
Pubmed Andersen,
Proteomic characterization of the human centrosome by protein correlation profiling.
2003,
Pubmed Avidor-Reiss,
Decoding cilia function: defining specialized genes required for compartmentalized cilia biogenesis.
2004,
Pubmed Baldari,
Intraflagellar transport: it's not just for cilia anymore.
2010,
Pubmed Becker,
Visualization of the cytoskeleton in Xenopus oocytes and eggs by confocal immunofluorescence microscopy.
2006,
Pubmed
,
Xenbase Berman,
A novel MAP kinase regulates flagellar length in Chlamydomonas.
2003,
Pubmed Burghoorn,
Mutation of the MAP kinase DYF-5 affects docking and undocking of kinesin-2 motors and reduces their speed in the cilia of Caenorhabditis elegans.
2007,
Pubmed Fariss,
Evidence from normal and degenerating photoreceptors that two outer segment integral membrane proteins have separate transport pathways.
1997,
Pubmed Finetti,
Intraflagellar transport is required for polarized recycling of the TCR/CD3 complex to the immune synapse.
2009,
Pubmed Frisch,
Development of order during ciliogenesis.
1968,
Pubmed Gherman,
The ciliary proteome database: an integrated community resource for the genetic and functional dissection of cilia.
2006,
Pubmed Glotzer,
The 3Ms of central spindle assembly: microtubules, motors and MAPs.
2009,
Pubmed Glotzer,
The molecular requirements for cytokinesis.
2005,
Pubmed Gray,
The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development.
2009,
Pubmed
,
Xenbase Gromley,
Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission.
2005,
Pubmed Hayes,
Identification of novel ciliogenesis factors using a new in vivo model for mucociliary epithelial development.
2007,
Pubmed
,
Xenbase Hedgecock,
Axonal guidance mutants of Caenorhabditis elegans identified by filling sensory neurons with fluorescein dyes.
1985,
Pubmed Horváth,
Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients.
2005,
Pubmed Hubbard,
Ensembl 2009.
2009,
Pubmed Inglis,
Piecing together a ciliome.
2006,
Pubmed Jiang,
PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis.
1998,
Pubmed Keller,
Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs of ciliary-disease genes.
2005,
Pubmed Kieserman,
Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesis.
2008,
Pubmed
,
Xenbase Kim,
MKKS/BBS6, a divergent chaperonin-like protein linked to the obesity disorder Bardet-Biedl syndrome, is a novel centrosomal component required for cytokinesis.
2005,
Pubmed Kim,
Functional genomic screen for modulators of ciliogenesis and cilium length.
2010,
Pubmed Kurasawa,
Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation.
2004,
Pubmed Lee,
Whole-mount fluorescence immunocytochemistry on Xenopus embryos.
2008,
Pubmed
,
Xenbase Lee,
A single gene network accurately predicts phenotypic effects of gene perturbation in Caenorhabditis elegans.
2008,
Pubmed Li,
Rational extension of the ribosome biogenesis pathway using network-guided genetics.
2009,
Pubmed Li,
Comparative genomics identifies a flagellar and basal body proteome that includes the BBS5 human disease gene.
2004,
Pubmed Liu,
The proteome of the mouse photoreceptor sensory cilium complex.
2007,
Pubmed Mahjoub,
A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells.
2004,
Pubmed Marshall,
The cell biological basis of ciliary disease.
2008,
Pubmed Mitchell,
A positive feedback mechanism governs the polarity and motion of motile cilia.
2007,
Pubmed
,
Xenbase Mostafavi,
GeneMANIA: a real-time multiple association network integration algorithm for predicting gene function.
2008,
Pubmed Mukhopadhyay,
Sensory signaling-dependent remodeling of olfactory cilia architecture in C. elegans.
2008,
Pubmed Mukhopadhyay,
Distinct IFT mechanisms contribute to the generation of ciliary structural diversity in C. elegans.
2007,
Pubmed Murayama,
The dyf-3 gene encodes a novel protein required for sensory cilium formation in Caenorhabditis elegans.
2005,
Pubmed Nachury,
A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis.
2007,
Pubmed Neef,
Choice of Plk1 docking partners during mitosis and cytokinesis is controlled by the activation state of Cdk1.
2007,
Pubmed Neugebauer,
FGF signalling during embryo development regulates cilia length in diverse epithelia.
2009,
Pubmed
,
Xenbase O'Connell,
A genetic screen for temperature-sensitive cell-division mutants of Caenorhabditis elegans.
1998,
Pubmed Pan,
RhoA-mediated apical actin enrichment is required for ciliogenesis and promoted by Foxj1.
2007,
Pubmed Park,
Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.
2008,
Pubmed
,
Xenbase Park,
Ciliogenesis defects in embryos lacking inturned or fuzzy function are associated with failure of planar cell polarity and Hedgehog signaling.
2006,
Pubmed
,
Xenbase Perkins,
Mutant sensory cilia in the nematode Caenorhabditis elegans.
1986,
Pubmed Potapova,
The reversibility of mitotic exit in vertebrate cells.
2006,
Pubmed
,
Xenbase Qin,
Intraflagellar transport protein 27 is a small G protein involved in cell-cycle control.
2007,
Pubmed Ren,
MiCroKit 3.0: an integrated database of midbody, centrosome and kinetochore.
2010,
Pubmed
,
Xenbase Shah,
Loss of Bardet-Biedl syndrome proteins alters the morphology and function of motile cilia in airway epithelia.
2008,
Pubmed Sharma,
Ciliary dysfunction in developmental abnormalities and diseases.
2008,
Pubmed Skop,
Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms.
2004,
Pubmed Sorokin,
Reconstructions of centriole formation and ciliogenesis in mammalian lungs.
1968,
Pubmed Spektor,
Cep97 and CP110 suppress a cilia assembly program.
2007,
Pubmed Steinman,
An electron microscopic study of ciliogenesis in developing epidermis and trachea in the embryo of Xenopus laevis.
1968,
Pubmed
,
Xenbase Swoboda,
The RFX-type transcription factor DAF-19 regulates sensory neuron cilium formation in C. elegans.
2000,
Pubmed Troemel,
Reprogramming chemotaxis responses: sensory neurons define olfactory preferences in C. elegans.
1997,
Pubmed Verbrugghe,
SPD-1 is required for the formation of the spindle midzone but is not essential for the completion of cytokinesis in C. elegans embryos.
2004,
Pubmed Yang,
The ciliary rootlet interacts with kinesin light chains and may provide a scaffold for kinesin-1 vesicular cargos.
2005,
Pubmed Zuo,
The exocyst protein Sec10 is necessary for primary ciliogenesis and cystogenesis in vitro.
2009,
Pubmed
