Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Xenopus laevis embryos are particularly well suited to address questions requiring either knockdown or overexpression of genes in a tissue-specific fashion during vertebrate embryonic development. These manipulations are achieved by targeted injection of either antisense morpholino oligonucleotides or synthetic mRNAs, respectively, into the early embryo. Herein we offer detailed protocols describing how to design and perform these experiments successfully, as well as a brief discussion of considerations for performing a microarray analysis in this organism.
Dale,
Fate map for the 32-cell stage of Xenopus laevis.
1987, Pubmed,
Xenbase
Dale,
Fate map for the 32-cell stage of Xenopus laevis.
1987,
Pubmed
,
Xenbase Dale,
Bone morphogenetic protein 4: a ventralizing factor in early Xenopus development.
1992,
Pubmed
,
Xenbase Galli,
Glypican 4 modulates FGF signalling and regulates dorsoventral forebrain patterning in Xenopus embryos.
2003,
Pubmed
,
Xenbase Green,
Slow emergence of a multithreshold response to activin requires cell-contact-dependent sharpening but not prepattern.
1994,
Pubmed
,
Xenbase Heasman,
Morpholino oligos: making sense of antisense?
2002,
Pubmed
,
Xenbase Jones,
DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase Kao,
Lithium-induced respecification of pattern in Xenopus laevis embryos.
,
Pubmed
,
Xenbase Lane,
Heading in a new direction: implications of the revised fate map for understanding Xenopus laevis development.
2006,
Pubmed
,
Xenbase Maéno,
A truncated bone morphogenetic protein 4 receptor alters the fate of ventral mesoderm to dorsal mesoderm: roles of animal pole tissue in the development of ventral mesoderm.
1994,
Pubmed
,
Xenbase Moody,
Fates of the blastomeres of the 16-cell stage Xenopus embryo.
1987,
Pubmed
,
Xenbase Moody,
Fates of the blastomeres of the 32-cell-stage Xenopus embryo.
1987,
Pubmed
,
Xenbase Ogino,
Resources and transgenesis techniques for functional genomics in Xenopus.
2009,
Pubmed
,
Xenbase Rupp,
Xenopus embryos regulate the nuclear localization of XMyoD.
1994,
Pubmed
,
Xenbase Sargent,
Analysis of class II gene regulation.
1991,
Pubmed
,
Xenbase Scharf,
Axis determination in eggs of Xenopus laevis: a critical period before first cleavage, identified by the common effects of cold, pressure and ultraviolet irradiation.
1983,
Pubmed
,
Xenbase Suzuki,
A truncated bone morphogenetic protein receptor affects dorsal-ventral patterning in the early Xenopus embryo.
1994,
Pubmed
,
Xenbase Turner,
Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate.
1994,
Pubmed
,
Xenbase Vize,
Assays for gene function in developing Xenopus embryos.
1991,
Pubmed
,
Xenbase Zelus,
Expression of the poly(A)-binding protein during development of Xenopus laevis.
1989,
Pubmed
,
Xenbase
