Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The error-free segregation of duplicated chromosomes during cell division is essential for the maintenance of an intact genome. This process is brought about by a highly dynamic bipolar array of microtubules, the mitotic spindle. The formation and function of the mitotic spindle during M-phase of the cell cycle is regulated by protein phosphorylation, involving multiple protein kinases and phosphatases. Prominent among the enzymes implicated in spindle assembly is the serine/threonine-specific protein kinase Aurora-A. In several common human tumors, Aurora-A is overexpressed, and deregulation of this kinase was shown to result in mitotic defects and aneuploidy. Moreover, recent genetic evidence directly links the human Aurora-A gene to cancer susceptibility. Several of the physiological substrates of Aurora-A presumably await identification, but recent studies are beginning to shed light on the regulation of this critical mitotic kinase. Here, we review these findings with particular emphasis on the role of TPX2, a prominent spindle component implicated in a Ran-GTP-mediated spindle assembly pathway.
Adams,
Chromosomal passengers and the (aurora) ABCs of mitosis.
2001, Pubmed
Adams,
Chromosomal passengers and the (aurora) ABCs of mitosis.
2001,
Pubmed Anand,
AURORA-A amplification overrides the mitotic spindle assembly checkpoint, inducing resistance to Taxol.
2003,
Pubmed Bayliss,
Structural basis of Aurora-A activation by TPX2 at the mitotic spindle.
2003,
Pubmed
,
Xenbase Bischoff,
A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers.
1998,
Pubmed Bischoff,
The Aurora/Ipl1p kinase family: regulators of chromosome segregation and cytokinesis.
1999,
Pubmed Bishop,
Phosphorylation of the carboxyl terminus of inner centromere protein (INCENP) by the Aurora B Kinase stimulates Aurora B kinase activity.
2002,
Pubmed Blagden,
Polar expeditions--provisioning the centrosome for mitosis.
2003,
Pubmed Dasso,
The Ran GTPase: theme and variations.
2002,
Pubmed Dikovskaya,
The adenomatous polyposis coli protein: in the limelight out at the edge.
2001,
Pubmed Ditchfield,
Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores.
2003,
Pubmed Dutertre,
On the role of aurora-A in centrosome function.
2002,
Pubmed Ewart-Toland,
Identification of Stk6/STK15 as a candidate low-penetrance tumor-susceptibility gene in mouse and human.
2003,
Pubmed Eyers,
A novel mechanism for activation of the protein kinase Aurora A.
2003,
Pubmed
,
Xenbase Goyal,
Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner.
1999,
Pubmed
,
Xenbase Gruss,
Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells.
2002,
Pubmed
,
Xenbase Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase Hauf,
The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint.
2003,
Pubmed Hirota,
Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells.
2003,
Pubmed
,
Xenbase Honda,
Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis.
2003,
Pubmed Kalab,
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts.
2002,
Pubmed
,
Xenbase Kanungo,
Ajuba, a cytosolic LIM protein, shuttles into the nucleus and affects embryonal cell proliferation and fate decisions.
2000,
Pubmed Katayama,
Interaction and feedback regulation between STK15/BTAK/Aurora-A kinase and protein phosphatase 1 through mitotic cell division cycle.
2001,
Pubmed Keryer,
Part of Ran is associated with AKAP450 at the centrosome: involvement in microtubule-organizing activity.
2003,
Pubmed
,
Xenbase Kimura,
Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.
1999,
Pubmed
,
Xenbase Kufer,
Human TPX2 is required for targeting Aurora-A kinase to the spindle.
2002,
Pubmed
,
Xenbase Littlepage,
Identification of phosphorylated residues that affect the activity of the mitotic kinase Aurora-A.
2002,
Pubmed
,
Xenbase Marie,
The LIM protein Ajuba is recruited to cadherin-dependent cell junctions through an association with alpha-catenin.
2003,
Pubmed Meraldi,
Aurora-A overexpression reveals tetraploidization as a major route to centrosome amplification in p53-/- cells.
2002,
Pubmed Nigg,
Mitotic kinases as regulators of cell division and its checkpoints.
2001,
Pubmed Roghi,
The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly.
1998,
Pubmed
,
Xenbase Sen,
A putative serine/threonine kinase encoding gene BTAK on chromosome 20q13 is amplified and overexpressed in human breast cancer cell lines.
1997,
Pubmed Shannon,
Chromosome dynamics: new light on Aurora B kinase function.
2002,
Pubmed Terada,
Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells.
2003,
Pubmed
,
Xenbase Tsai,
A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly.
2003,
Pubmed
,
Xenbase Walter,
The mitotic serine/threonine kinase Aurora2/AIK is regulated by phosphorylation and degradation.
2000,
Pubmed Wittmann,
Localization of the kinesin-like protein Xklp2 to spindle poles requires a leucine zipper, a microtubule-associated protein, and dynein.
1998,
Pubmed
,
Xenbase Wittmann,
TPX2, A novel xenopus MAP involved in spindle pole organization.
2000,
Pubmed
,
Xenbase Zhou,
Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation.
1998,
Pubmed
