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Differentiated cells do not revert to an embryonic state in normal development. However, the method called nuclear reprogramming enables these differentiated cells to be reversed to an embryonic state. One essential event in the reprogramming process is reactivation of embryonic genes such as Oct4 (also known as Pou5f1). This reprogramming of transcriptional programs can be achieved by transplantation of mammalian somatic nuclei to the giant Xenopus laevis oocytenucleus, referred to as the germinal vesicle (GV). Factors and mechanisms responsible for this transcriptional reprogramming have not been elucidated. Recently, we have found that a polymerized form of actin is abundantly present in nuclei transplanted into the Xenopus oocytenucleus and plays an important role in transcriptional reactivation of Oct4. This study emphasizes a significant contribution of nuclear actin in transcriptional activation. Here, we discuss possible roles of nuclear actin in Xenopus oocytes and in other cell types in the context of transcriptional activation.
Bhutani,
Reprogramming towards pluripotency requires AID-dependent DNA demethylation.
2010, Pubmed
Bhutani,
Reprogramming towards pluripotency requires AID-dependent DNA demethylation.
2010,
Pubmed Bohnsack,
A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes.
2006,
Pubmed
,
Xenbase Byrne,
Nuclei of adult mammalian somatic cells are directly reprogrammed to oct-4 stem cell gene expression by amphibian oocytes.
2003,
Pubmed
,
Xenbase Chuang,
Long-range directional movement of an interphase chromosome site.
2006,
Pubmed Clark,
Diffusible and bound actin nuclei of Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase Dundr,
Actin-dependent intranuclear repositioning of an active gene locus in vivo.
2007,
Pubmed Ferrai,
Induction of HoxB transcription by retinoic acid requires actin polymerization.
2009,
Pubmed Gall,
Structure in the amphibian germinal vesicle.
2004,
Pubmed
,
Xenbase Gieni,
Actin dynamics and functions in the interphase nucleus: moving toward an understanding of nuclear polymeric actin.
2009,
Pubmed GURDON,
Sexually mature individuals of Xenopus laevis from the transplantation of single somatic nuclei.
1958,
Pubmed
,
Xenbase Halley-Stott,
Mammalian nuclear transplantation to Germinal Vesicle stage Xenopus oocytes - a method for quantitative transcriptional reprogramming.
2010,
Pubmed
,
Xenbase Huang,
Coronin 2A mediates actin-dependent de-repression of inflammatory response genes.
2011,
Pubmed Jullien,
Characterization of somatic cell nuclear reprogramming by oocytes in which a linker histone is required for pluripotency gene reactivation.
2010,
Pubmed
,
Xenbase Kim,
Epigenetic memory in induced pluripotent stem cells.
2010,
Pubmed Koziol,
Tpt1 activates transcription of oct4 and nanog in transplanted somatic nuclei.
2007,
Pubmed
,
Xenbase Miyamoto,
Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes.
2011,
Pubmed
,
Xenbase Pereira,
ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency.
2010,
Pubmed Scheer,
Microinjection of actin-binding proteins and actin antibodies demonstrates involvement of nuclear actin in transcription of lampbrush chromosomes.
1984,
Pubmed Simonsson,
DNA demethylation is necessary for the epigenetic reprogramming of somatic cell nuclei.
2004,
Pubmed
,
Xenbase Tada,
Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells.
2001,
Pubmed Takahashi,
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
2006,
Pubmed Taylor,
Nuclear role of WASp in the pathogenesis of dysregulated TH1 immunity in human Wiskott-Aldrich syndrome.
2010,
Pubmed Visa,
Nuclear functions of actin.
2010,
Pubmed Wilmut,
Viable offspring derived from fetal and adult mammalian cells.
1997,
Pubmed Xu,
Nuclear translocation of beta-actin is involved in transcriptional regulation during macrophage differentiation of HL-60 cells.
2010,
Pubmed Zhao,
Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling.
1998,
Pubmed
