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Xenopus laevis provides a unique animal model, alternative to mouse, to study immunology. Even though, several methodologies have been developed for the generation of transgenic Xenopus, to date none have been adapted for the X. laevis/gilli (LG) isogenetic clones that are essential for immunological studies. Since LG clones are generated via gynogenesis, transgenic methods using transgene integration into the sperm nuclei are not suited. Therefore, we have tested three alternative methods for LG transgenesis: the phiC31 integrase, the Sleeping Beauty transposase, and the I-SceI meganuclease. All three techniques produced transgenic LG clones; however, the I-SceI meganuclease was most effective. It resulted in high transgenesis efficiency (35-50%), bright nonmosaic GFP expression as well as stable germline transmission with 100% of the progeny carrying the transgene. Production of transgenic LG clones will allow us to modulate immune gene expression and further strengthen X. laevis as a biomedical model.
Allen,
Transgenic Xenopus laevis embryos can be generated using phiC31 integrase.
2005, Pubmed,
Xenbase
Allen,
Transgenic Xenopus laevis embryos can be generated using phiC31 integrase.
2005,
Pubmed
,
Xenbase Amaya,
A method for generating transgenic frog embryos.
1999,
Pubmed
,
Xenbase Bowers,
Neuronal precursor-restricted transduction via in utero CNS gene delivery of a novel bipartite HSV amplicon/transposase hybrid vector.
2006,
Pubmed Doherty,
A flk-1 promoter/enhancer reporter transgenic Xenopus laevis generated using the Sleeping Beauty transposon system: an in vivo model for vascular studies.
2007,
Pubmed
,
Xenbase Du Pasquier,
The immune system of Xenopus.
1989,
Pubmed
,
Xenbase Groth,
A phage integrase directs efficient site-specific integration in human cells.
2000,
Pubmed Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase Nedelkovska,
Comparative in vivo study of gp96 adjuvanticity in the frog Xenopus laevis.
2010,
Pubmed
,
Xenbase Ogino,
Highly efficient transgenesis in Xenopus tropicalis using I-SceI meganuclease.
2006,
Pubmed
,
Xenbase Pan,
I-SceI meganuclease-mediated transgenesis in Xenopus.
2006,
Pubmed
,
Xenbase Robert,
Xenopus as an experimental model for studying evolution of hsp--immune system interactions.
2004,
Pubmed
,
Xenbase Robert,
Minor histocompatibility antigen-specific MHC-restricted CD8 T cell responses elicited by heat shock proteins.
2002,
Pubmed
,
Xenbase Robert,
Lymphoid tumors of Xenopus laevis with different capacities for growth in larvae and adults.
1994,
Pubmed
,
Xenbase Robert,
Ontogeny of the alloimmune response against a transplanted tumor in Xenopus laevis.
1995,
Pubmed
,
Xenbase Robert,
Evolution of immune surveillance and tumor immunity: studies in Xenopus.
1998,
Pubmed
,
Xenbase Robert,
Comparative and developmental study of the immune system in Xenopus.
2009,
Pubmed
,
Xenbase Sinzelle,
Generation of trangenic Xenopus laevis using the Sleeping Beauty transposon system.
2006,
Pubmed
,
Xenbase Thermes,
I-SceI meganuclease mediates highly efficient transgenesis in fish.
2002,
Pubmed Thyagarajan,
Site-specific genomic integration in mammalian cells mediated by phage phiC31 integrase.
2001,
Pubmed Yergeau,
Transgenesis in Xenopus using the Sleeping Beauty transposon system.
2009,
Pubmed
,
Xenbase
