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Structure and assembly mechanism for heteromeric kainate receptors.
Kumar J, Schuck P, Mayer ML.
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Native glutamate receptor ion channels are tetrameric assemblies containing two or more different subunits. NMDA receptors are obligate heteromers formed by coassembly of two or three divergent gene families. While some AMPA and kainate receptors can form functional homomeric ion channels, the KA1 and KA2 subunits are obligate heteromers which function only in combination with GluR5-7. The mechanisms controlling glutamate receptor assembly involve an initial step in which the amino terminal domains (ATD) assemble as dimers. Here, we establish by sedimentation velocity that the ATDs of GluR6 and KA2 coassemble as a heterodimer of K(d) 11 nM, 32,000-fold lower than the K(d) for homodimer formation by KA2; we solve crystal structures for the GluR6/KA2 ATD heterodimer and heterotetramer assemblies. Using these structures as a guide, we perform a mutant cycle analysis to probe the energetics of assembly and show that high-affinity ATD interactions are required for biosynthesis of functional heteromeric receptors.
Adams,
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
2010, Pubmed
Adams,
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
2010,
Pubmed Ayalon,
Functional assembly of AMPA and kainate receptors is mediated by several discrete protein-protein interactions.
2001,
Pubmed
,
Xenbase Ayalon,
Two regions in the N-terminal domain of ionotropic glutamate receptor 3 form the subunit oligomerization interfaces that control subtype-specific receptor assembly.
2005,
Pubmed Brose,
Differential assembly of coexpressed glutamate receptor subunits in neurons of rat cerebral cortex.
1994,
Pubmed Brown,
Characterizing protein-protein interactions by sedimentation velocity analytical ultracentrifugation.
2008,
Pubmed Burnashev,
Divalent ion permeability of AMPA receptor channels is dominated by the edited form of a single subunit.
1992,
Pubmed Carter,
The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus).
1984,
Pubmed Chen,
Functional characterization of a potassium-selective prokaryotic glutamate receptor.
1999,
Pubmed
,
Xenbase Clayton,
Crystal structure of the GluR2 amino-terminal domain provides insights into the architecture and assembly of ionotropic glutamate receptors.
2009,
Pubmed Dalva,
EphB receptors interact with NMDA receptors and regulate excitatory synapse formation.
2000,
Pubmed Das,
Domain organization and function in GluK2 subtype kainate receptors.
2010,
Pubmed Egebjerg,
Cloning of a cDNA for a glutamate receptor subunit activated by kainate but not AMPA.
1991,
Pubmed
,
Xenbase Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed Farina,
Separation of domain contacts is required for heterotetrameric assembly of functional NMDA receptors.
2011,
Pubmed Furukawa,
Subunit arrangement and function in NMDA receptors.
2005,
Pubmed Geiger,
Relative abundance of subunit mRNAs determines gating and Ca2+ permeability of AMPA receptors in principal neurons and interneurons in rat CNS.
1995,
Pubmed Gielen,
Mechanism of differential control of NMDA receptor activity by NR2 subunits.
2009,
Pubmed Greger,
AMPA receptor biogenesis and trafficking.
2007,
Pubmed Hansen,
How glutamate receptor subunits mix and match: details uncovered.
2011,
Pubmed Herb,
The KA-2 subunit of excitatory amino acid receptors shows widespread expression in brain and forms ion channels with distantly related subunits.
1992,
Pubmed Hidalgo,
Revealing the architecture of a K+ channel pore through mutant cycles with a peptide inhibitor.
1995,
Pubmed
,
Xenbase Hollmann,
Cloning by functional expression of a member of the glutamate receptor family.
1989,
Pubmed
,
Xenbase Horning,
Regulation of AMPA receptor gating by ligand binding core dimers.
2004,
Pubmed Jin,
Crystal structure and association behaviour of the GluR2 amino-terminal domain.
2009,
Pubmed Karakas,
Structure of the zinc-bound amino-terminal domain of the NMDA receptor NR2B subunit.
2009,
Pubmed
,
Xenbase Keinänen,
A family of AMPA-selective glutamate receptors.
1990,
Pubmed Kumar,
Crystal structures of the glutamate receptor ion channel GluK3 and GluK5 amino-terminal domains.
2010,
Pubmed Kumar,
The N-terminal domain of GluR6-subtype glutamate receptor ion channels.
2009,
Pubmed Leuschner,
Subtype-specific assembly of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits is mediated by their n-terminal domains.
1999,
Pubmed Mah,
Glutamate receptor trafficking: endoplasmic reticulum quality control involves ligand binding and receptor function.
2005,
Pubmed Matsuda,
Cbln1 is a ligand for an orphan glutamate receptor delta2, a bidirectional synapse organizer.
2010,
Pubmed McCoy,
Phaser crystallographic software.
2007,
Pubmed Monyer,
Heteromeric NMDA receptors: molecular and functional distinction of subtypes.
1992,
Pubmed O'Brien,
Synaptic clustering of AMPA receptors by the extracellular immediate-early gene product Narp.
1999,
Pubmed Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed Otwinowski,
[20] Processing of X-ray diffraction data collected in oscillation mode.
2019,
Pubmed Partin,
Selective modulation of desensitization at AMPA versus kainate receptors by cyclothiazide and concanavalin A.
1993,
Pubmed
,
Xenbase Pasternack,
Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor channels lacking the N-terminal domain.
2002,
Pubmed Penn,
Gating motions underlie AMPA receptor secretion from the endoplasmic reticulum.
2008,
Pubmed Petralia,
Histological and ultrastructural localization of the kainate receptor subunits, KA2 and GluR6/7, in the rat nervous system using selective antipeptide antibodies.
1994,
Pubmed Plested,
Structure and mechanism of kainate receptor modulation by anions.
2007,
Pubmed Reeves,
Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line.
2002,
Pubmed Ren,
Multiple trafficking signals regulate kainate receptor KA2 subunit surface expression.
2003,
Pubmed Ripley,
Regulation of synaptic stability by AMPA receptor reverse signaling.
2011,
Pubmed Rossmann,
Subunit-selective N-terminal domain associations organize the formation of AMPA receptor heteromers.
2011,
Pubmed Saglietti,
Extracellular interactions between GluR2 and N-cadherin in spine regulation.
2007,
Pubmed Schiffer,
Rat GluR7 and a carboxy-terminal splice variant, GluR7b, are functional kainate receptor subunits with a low sensitivity to glutamate.
1997,
Pubmed Schröder,
Super-resolution biomolecular crystallography with low-resolution data.
2010,
Pubmed Schuck,
Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling.
2000,
Pubmed Schuck,
On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation.
2003,
Pubmed Shanks,
Contribution of the global subunit structure and stargazin on the maturation of AMPA receptors.
2010,
Pubmed Shi,
Functional comparison of the effects of TARPs and cornichons on AMPA receptor trafficking and gating.
2010,
Pubmed Sia,
Interaction of the N-terminal domain of the AMPA receptor GluR4 subunit with the neuronal pentraxin NP1 mediates GluR4 synaptic recruitment.
2007,
Pubmed Sobolevsky,
X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor.
2009,
Pubmed Swanson,
Kainate receptors exhibit differential sensitivities to (S)-5-iodowillardiine.
1998,
Pubmed Takasu,
Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors.
2002,
Pubmed Traynelis,
Glutamate receptor ion channels: structure, regulation, and function.
2010,
Pubmed Uemura,
Trans-synaptic interaction of GluRdelta2 and Neurexin through Cbln1 mediates synapse formation in the cerebellum.
2010,
Pubmed Valluru,
Ligand binding is a critical requirement for plasma membrane expression of heteromeric kainate receptors.
2005,
Pubmed Vistica,
Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decomposition.
2004,
Pubmed Watkins,
Excitatory amino acid transmitters.
1981,
Pubmed Werner,
Cloning of a putative high-affinity kainate receptor expressed predominantly in hippocampal CA3 cells.
1991,
Pubmed Xu,
Narp and NP1 form heterocomplexes that function in developmental and activity-dependent synaptic plasticity.
2003,
Pubmed Yuan,
Control of NMDA receptor function by the NR2 subunit amino-terminal domain.
2009,
Pubmed
,
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