Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2011 Nov 01;3122:4623-32. doi: 10.1128/MCB.05715-11.
Show Gene links
Show Anatomy links
Nucleosome disruption by DNA ligase III-XRCC1 promotes efficient base excision repair.
Odell ID, Barbour JE, Murphy DL, Della-Maria JA, Sweasy JB, Tomkinson AE, Wallace SS, Pederson DS.
???displayArticle.abstract???
Each day, approximately 20,000 oxidative lesions form in the DNA of every nucleated human cell. The base excision repair (BER) enzymes that repair these lesions must function in a chromatin milieu. We have determined that the DNA glycosylase hNTH1, apurinic endonuclease (APE), and DNA polymerase β (Pol β), which catalyze the first three steps in BER, are able to process their substrates in both 601- and 5S ribosomal DNA (rDNA)-based nucleosomes. hNTH1 formed a discrete ternary complex that was displaced by the addition of APE, suggesting an orderly handoff of substrates from one enzyme to the next. In contrast, DNA ligase IIIα-XRCC1, which completes BER, was appreciably active only at concentrations that led to nucleosome disruption. Ligase IIIα-XRCC1 was also able to bind and disrupt nucleosomes containing a single base gap and, because of this property, enhanced both its own activity and that of Pol β on nucleosome substrates. Collectively, these findings provide insights into rate-limiting steps that govern BER in chromatin and reveal a unique role for ligase IIIα-XRCC1 in enhancing the efficiency of the final two steps in the BER of lesions in nucleosomes.
Almeida,
A unified view of base excision repair: lesion-dependent protein complexes regulated by post-translational modification.
2007, Pubmed
Almeida,
A unified view of base excision repair: lesion-dependent protein complexes regulated by post-translational modification.
2007,
Pubmed Anderson,
Sequence and position-dependence of the equilibrium accessibility of nucleosomal DNA target sites.
2000,
Pubmed Beard,
DNA polymerase beta substrate specificity: side chain modulation of the "A-rule".
2009,
Pubmed Beard,
Suppressed catalytic activity of base excision repair enzymes on rotationally positioned uracil in nucleosomes.
2003,
Pubmed Blaisdell,
Rapid determination of the active fraction of DNA repair glycosylases: a novel fluorescence assay for trapped intermediates.
2007,
Pubmed Breen,
Reactions of oxyl radicals with DNA.
1995,
Pubmed Chafin,
Human DNA ligase I efficiently seals nicks in nucleosomes.
2000,
Pubmed
,
Xenbase Cotner-Gohara,
Human DNA ligase III recognizes DNA ends by dynamic switching between two DNA-bound states.
2010,
Pubmed
,
Xenbase Cuneo,
Oxidation state of the XRCC1 N-terminal domain regulates DNA polymerase beta binding affinity.
2010,
Pubmed David,
Base-excision repair of oxidative DNA damage.
2007,
Pubmed Dong,
DNA and protein determinants of nucleosome positioning on sea urchin 5S rRNA gene sequences in vitro.
1990,
Pubmed Dyer,
Reconstitution of nucleosome core particles from recombinant histones and DNA.
2004,
Pubmed Engelward,
Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase.
1997,
Pubmed Enright,
Preferential targeting of oxidative base damage to internucleosomal DNA.
1996,
Pubmed Flaus,
Mapping nucleosome position at single base-pair resolution by using site-directed hydroxyl radicals.
1996,
Pubmed Fromme,
Structure of a trapped endonuclease III-DNA covalent intermediate.
2003,
Pubmed Gao,
DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair.
2011,
Pubmed
,
Xenbase Groth,
Chromatin challenges during DNA replication and repair.
2007,
Pubmed Gryk,
Mapping of the interaction interface of DNA polymerase beta with XRCC1.
2002,
Pubmed Hayes,
The structure of DNA in a nucleosome.
1990,
Pubmed
,
Xenbase Hegde,
Early steps in the DNA base excision/single-strand interruption repair pathway in mammalian cells.
2008,
Pubmed Hinz,
Rotational dynamics of DNA on the nucleosome surface markedly impact accessibility to a DNA repair enzyme.
2010,
Pubmed Huffman,
DNA base damage recognition and removal: new twists and grooves.
2005,
Pubmed Huggins,
Flap endonuclease 1 efficiently cleaves base excision repair and DNA replication intermediates assembled into nucleosomes.
2002,
Pubmed
,
Xenbase Lang,
A DNA polymerase beta mutant from colon cancer cells induces mutations.
2004,
Pubmed Li,
Rapid spontaneous accessibility of nucleosomal DNA.
2005,
Pubmed Liu,
In vitro and in vivo dimerization of human endonuclease III stimulates its activity.
2003,
Pubmed Ljungman,
Efficient protection against oxidative DNA damage in chromatin.
1992,
Pubmed Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase McGhee,
Reaction of nucleosome DNA with dimethyl sulfate.
1979,
Pubmed Menoni,
ATP-dependent chromatin remodeling is required for base excision repair in conventional but not in variant H2A.Bbd nucleosomes.
2007,
Pubmed Mol,
DNA-bound structures and mutants reveal abasic DNA binding by APE1 and DNA repair coordination [corrected].
2000,
Pubmed Murphy,
A triad interaction in the fingers subdomain of DNA polymerase beta controls polymerase activity.
2011,
Pubmed Nakanishi,
Different structural states in oligonucleosomes are required for early versus late steps of base excision repair.
2007,
Pubmed Nilsen,
DNA base excision repair of uracil residues in reconstituted nucleosome core particles.
2002,
Pubmed Odell,
Non-specific DNA binding interferes with the efficient excision of oxidative lesions from chromatin by the human DNA glycosylase, NEIL1.
2010,
Pubmed Palomera-Sanchez,
Open, repair and close again: chromatin dynamics and the response to UV-induced DNA damage.
2011,
Pubmed Parikh,
Envisioning the molecular choreography of DNA base excision repair.
1999,
Pubmed Pascal,
Human DNA ligase I completely encircles and partially unwinds nicked DNA.
2004,
Pubmed Pennings,
Mobility of positioned nucleosomes on 5 S rDNA.
1991,
Pubmed Petermann,
Roles of DNA ligase III and XRCC1 in regulating the switch between short patch and long patch BER.
2006,
Pubmed Prasad,
Substrate channeling in mammalian base excision repair pathways: passing the baton.
2010,
Pubmed Prasad,
Initiation of base excision repair of oxidative lesions in nucleosomes by the human, bifunctional DNA glycosylase NTH1.
2007,
Pubmed Schalch,
X-ray structure of a tetranucleosome and its implications for the chromatin fibre.
2005,
Pubmed
,
Xenbase Simpson,
Structural features of a phased nucleosome core particle.
1983,
Pubmed Slupphaug,
A nucleotide-flipping mechanism from the structure of human uracil-DNA glycosylase bound to DNA.
1996,
Pubmed Sobol,
DNA polymerase beta null mouse embryonic fibroblasts harbor a homozygous null mutation in DNA polymerase iota.
2007,
Pubmed Thåström,
Sequence motifs and free energies of selected natural and non-natural nucleosome positioning DNA sequences.
1999,
Pubmed Thåström,
Measurement of histone-DNA interaction free energy in nucleosomes.
2004,
Pubmed Wallace,
The enigma of endonuclease VIII.
2003,
Pubmed Waters,
Human thymine DNA glycosylase binds to apurinic sites in DNA but is displaced by human apurinic endonuclease 1.
1999,
Pubmed Wilson,
Passing the baton in base excision repair.
2000,
Pubmed Wilson,
Repair mechanisms for oxidative DNA damage.
2003,
Pubmed
